A5067195079- Magnetic properties of thin films
- Magnetic Properties and Applications
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Magneto-Optical Properties and Applications
- Immune Cell Function and Interaction
- Magnetic Properties of Alloys
- Rheumatoid Arthritis Research and Therapies
- Heusler alloys: electronic and magnetic properties
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Magnetic and transport properties of perovskites and related materials
- Vasculitis and related conditions
- Cytokine Signaling Pathways and Interactions
- Metallic Glasses and Amorphous Alloys
- Magnetic Properties and Synthesis of Ferrites
- Immunodeficiency and Autoimmune Disorders
- ZnO doping and properties
- Theoretical and Computational Physics
- Lymphoma Diagnosis and Treatment
- Multiferroics and related materials
- Autoimmune and Inflammatory Disorders Research
- Nuclear physics research studies
- Physics of Superconductivity and Magnetism
- Psoriasis: Treatment and Pathogenesis
Wakayama Medical University
2022-2025
Kitasato University
2025
University of Occupational and Environmental Health Japan
2015-2024
Saiseikai Shimonoseki General Hospital
2023
Nagoya Industrial Science Research Institute
2021
Nagoya University
2010-2019
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2014-2017
National Institutes of Health
2014-2017
Paul Scherrer Institute
2004
Kyushu University
2004
<b>Aim:</b> Neuropsychiatric systemic lupus erythematosus (NPSLE) is a serious treatment-resistant phenotype of erythematosus. A standard treatment for NPSLE not available. This report describes the clinical and laboratory tests 10 patients with before after rituximab treatment, including changes in lymphocyte phenotypes. <b>Methods:</b> Rituximab was administered at different doses refractory NPSLE, despite intensive treatment. <b>Results:</b> Treatment resulted rapid improvement central...
Thiol redox status modulates various aspects of cellular function. We demonstrate that oxidation sulfhydryl (SH) groups induces apoptosis. In Jurkat T cells and human PBL blasts, the fraction apoptotic nuclei increased after treatment with an SH-specific oxidant, diamide. Analysis DNA fragmentation nuclear morphology also indicated SH could induce apoptosis induced by oxidation, decrease glutathione was transient increase disulfide observed only changes had occurred. Depletion buthionine...
Tofacitinib (CP-690,550) is a novel JAK inhibitor that currently in clinical trials for the treatment of rheumatoid arthritis (RA). The aim this study was to examine effects tofacitinib vitro and vivo RA, order elucidate role disease process.CD4+ T cells, CD14+ monocytes, synovial fibroblasts (SFs) were purified from synovium peripheral blood patients with RA evaluated effect on cytokine production cell proliferation. For analysis, cartilage samples obtained implanted immunodeficient mice...
Thioredoxin is a small ubiquitous protein with multiple biological functions, including cellular defense mechanisms against oxidative stress. In the present study, we investigated role of human thioredoxin (hTRX) in acquisition resistance to cis-diamminedichloroplatinum (II) (CDDP). The expression and activity hTRX Jurkat T cells was dose-dependently enhanced by exposure CDDP, as determined immunoblot analysis insulin reducing assay. Furthermore, chloramphenicol acetyltransferase using...
Tofacitinib, which is a Janus kinase (JAK) inhibitor, has shown clinical effects in the treatment of rheumatoid arthritis. JAKs are important kinases lymphocyte differentiation; however, their function dendritic cells (DCs) unknown. In this study, DCs was investigated with tofacitinib.The tofacitinib on maturation human monocyte-derived induced by lipopolysaccharide (LPS) stimulation were investigated. addition, its T cell stimulatory capability coculturing naïve CD45RA-positive...
CD4(+) T follicular helper cells (TFH) are critical for the formation and function of B cell responses to infection or immunization, but also play an important role in autoimmunity. The factors that contribute differentiation this subset incompletely understood, although several cytokines including IL-6, IL-21, IL-12 can promote TFH formation. Yet, none these factors, nor their downstream cognate STATs, have emerged as nonredundant, essential drivers cells. This suggests a model which...
We sought to investigate the selection of specific biological DMARDs (bDMARDs) based on characteristic lymphocyte phenotypes for treating PsA.Of 64 patients with PsA resistant MTX, 26 underwent bDMARDs therapy selected according phenotypic differences in peripheral helper T cells 8-colour flow cytometry. The efficacies this strategic treatment and standard administered other 38 were evaluated at 6 months.The group classified into following four types blood analysis: (i)...
Objective To elucidate the diversity of systemic lupus erythematosus (SLE) based on immunophenotyping. Methods Peripheral blood mononuclear cells were obtained from 143 SLE patients and 49 healthy individuals. Circulating B, T, dendritic defined using flow cytometric analysis as recommended by Human Immunology Project Consortium. Based these results, immunophenotypes distinguished principal components (PCA), cluster was used to classify into subgroups. Results The proportions Treg follicular...
Innate lymphoid cells (ILCs) patrol environmental interfaces to defend against infection and protect barrier integrity. Using a genetic tuning model, we demonstrate that the signal-dependent transcription factor (TF) STAT5 is critical for accumulation of all known ILC subsets in mice reveal hierarchy dependency populating nonlymphoid tissues. We apply transcriptome genomic distribution analyses define gene signature natural killer (NK) cells, prototypical subset, provide systems-based...
CD40, a TNF receptor family member, plays central role in T cell-mediated B cell activation. We have recently demonstrated that CD27, another was also involved regulation and enhanced Ig production. In this report we compare CD27 CD40 signals function. selectively mimicked the effect of help by addition to peripheral blood cells activated with Staphylococcus aureus Cowan I strain IL-2 irradiated 300-19 transfected either CD70 (CD27 ligand) gene or CD154 (CD40 gene, vector alone, both genes....
B cells play a crucial role in the pathogenesis of autoimmune diseases, such as systemic lupus erythematosus (SLE). However, relevance metabolic pathway differentiation human cell subsets remains unknown. In this article, we show that combination CpG/TLR9 and IFN-α markedly induced CD27+IgD+ unswitched memory into CD27hiCD38hi plasmablasts. The response was accompanied by mammalian target rapamycin complex 1 (mTORC1) activation increased lactate production, indicating shift to glycolysis....
The aim of this study was to assess the therapeutic effects biological DMARDs (bDMARDs) on diversity immune cell phenotypes in peripheral blood patients with RA. Peripheral were determined 108 RA who non-responsive conventional and 33 healthy control subjects by eight-colour flow cytometry. We also examined correlation between clinical findings assessed 24-week treatment bDMARDs. proportions T follicular helper (Tfh) cells, IgD− CD27− double negative B cells plasmacytoid dendritic (pDCs)...
B cells play a pivotal role in the pathogenesis of autoimmune diseases. In patients with systemic lupus erythematosus (SLE), percentages plasmablasts and IgD − CD27 double-negative memory peripheral blood are significantly increased, while + IgM decreased compared to healthy donors. The phenotypic change is associated disease activity concentration autoantibodies. Treatment B-cell depletion using rituximab results reconstitution SLE subsequent improvement activity. Numerous studies have...
T helper (Th) cells can differentiate into functionally distinct subsets and play a pivotal role in inflammatory autoimmune diseases such as rheumatoid arthritis (RA). Th22 have been identified new subset secreting interleukin (IL)-22. Although elevated levels of IL-22 the synovial fluids RA patients were reported, its pathological roles remain unclear. Here, we demonstrated that was characteristically produced from CD3+CD4+CC-chemokine receptor (CCR)4+CCR6+CCR10+ their ability production...
Objective B cells play a pivotal role in the pathogenesis of autoimmune diseases. Although Syk functions as key molecule cell receptor signaling, pathologic rheumatoid arthritis (RA) remains unclear. The purpose this study was to assess relevance activation development RA and responsiveness patients treatment with biologics. Methods Healthy subjects (n = 36) moderate or severe disease activity 70) were studied. phosphorylation (pSyk) peripheral blood measured by flow cytometry, its...
This study was undertaken to identify characteristics of follicular regulatory T (Tfr) cells and elucidate the mechanisms by which helper (Tfh) convert Tfr cells. We probed phenotype in patients with systemic lupus erythematosus (SLE) underlying transcriptional regulation using cytokine-induced STAT family factors.Peripheral blood mononuclear from 41 SLE 26 healthy donors were used sort out memory Tfh cell subset, cultured under various conditions. The flow cytometry quantitative polymerase...
This study aimed to clarify the usefulness of screening for malignancies using CT before initiation biologic and targeted synthetic DMARDs (b/tsDMARDs) in patients with active RA.We examined 2192 RA who underwent plain scans prior b/tsDMARDs. The sensitivity detecting malignancy was measured compared that regular (physical examination X-ray). We then evaluated clinical characteristics, prognosis treatment concomitant malignancies. Additionally, we determined incidence rate were initiated on...
Thiol compounds, such as L-cysteine and glutathione (GSH), play crucial roles in the regulation of lymphocyte proliferation. In this study, we analyzed effect L-cystine GSH depletion on survival investigated regulatory adult T cell leukemia (ATL)-derived factor (ADF)/human thioredoxin (hTRX) relation to these low m.w. thiols. MT-1, MT-2, Jurkat cells underwent apoptosis when cultured L-cystine- GSH-free medium within 18 24 h. Dichlorofluorescin oxidation assay indicated that MT-1 MT-2 was...
Abstract The proliferative response of PBMC to PHA, Con A, OKT3 mAb and IL-2-dependent proliferation PHA-blasts was examined in a thiol-free environment (cultured L-cystine- GSH-free medium). [3H]TdR incorporation assay cell cycle analysis revealed that stimulated could not enter the S phase when deprived these thiol compounds. In cultures, an increase intracellular free Ca2+ concentration IL-2R alpha-chain/p 55 (Tac) induction still observed, whereas transferrin receptor markedly reduced,...
It is often difficult to manage refractory gastrointestinal tract complications of Behçet's disease (entero-BD) by conventional therapy. In this study, we assessed the short- and long-term efficacy safety combination therapy infliximab, an anti-tumor-necrosis-factor (TNF)-α antibody, methotrexate in ten patients with entero-BD therapies. The (weeks) (by 2 years) effects infliximab at 3-5 mg/kg body weight every 8 weeks on clinical course intestinal manifestations were abdominal computed...