A5103211341- Mosquito-borne diseases and control
- Ultrasound Imaging and Elastography
- Virology and Viral Diseases
- Ultrasound and Hyperthermia Applications
- Viral Infections and Vectors
- Global Maternal and Child Health
- Photoacoustic and Ultrasonic Imaging
- Neonatal and fetal brain pathology
- Cerebral Venous Sinus Thrombosis
- Facial Nerve Paralysis Treatment and Research
- Vestibular and auditory disorders
- Pathogenesis and Treatment of Hiccups
- Prenatal Screening and Diagnostics
- Bone health and osteoporosis research
- Pleural and Pulmonary Diseases
- Autism Spectrum Disorder Research
- Meningioma and schwannoma management
- Parvovirus B19 Infection Studies
- Preterm Birth and Chorioamnionitis
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Trigeminal Neuralgia and Treatments
University of Wisconsin–Madison
2013-2022
Infection with Zika virus (ZIKV) is associated human congenital fetal anomalies. To model outcomes in nonhuman primates, we administered Asian-lineage ZIKV subcutaneously to four pregnant rhesus macaques. While non-pregnant animals a previous study contemporary the current report clear viremia within 10–12 days, maternal was prolonged 3 of 4 pregnancies. Fetal head growth velocity last month gestation determined by ultrasound assessment circumference decreased comparison biparietal diameter...
An interlaboratory study of shear wave speed (SWS) estimation was performed. Commercial elastography systems from Fibroscan, Philips, Siemens and Supersonic Imagine, as well several custom laboratory systems, were involved. Fifteen sites included in the study. CIRS manufactured donated 11 pairs phantoms designed for purposes this investigation. Dynamic mechanical tests equivalent phantom materials also The results demonstrate that there is very good agreement among SWS but are sources bias...
Congenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with Puerto Rican ZIKV isolate in the first trimester. The was complicated by preterm premature rupture of membranes (PPROM), intraamniotic bacterial demise 49 days post (gestational day 95). Significant pathology at maternal-fetal interface included acute chorioamnionitis, placental infarcts, leukocytoclastic vasculitis myometrial radial arteries. RNA disseminated...
Ultrasound elasticity imaging has demonstrated utility in breast imaging, but it is typically performed with handheld transducers and two-dimensional imaging. Two-dimensional (2D) elastography images tissue stiffness of only a plane hence suffers from errors due to out-of-plane motion, whereas three-dimensional (3D) data acquisition motion tracking can be used track that lost 2D systems. A commercially available automated volume scanning system acquires 3D ultrasound precisely controlled...
Congenital Zika virus (ZIKV) exposure results in a spectrum of disease ranging from severe birth defects to delayed onset neurodevelopmental deficits. ZIKV-related neuropathogenesis, predictors defects, and deficits are not well defined people. Here we assess the methodological statistical feasibility congenital ZIKV macaque model for identifying infant neurobehavior brain abnormalities that may underlie We inoculated five pregnant macaques with mock-inoculated one first trimester. Following...
There are currently no approved drugs to treat Zika virus (ZIKV) infection during pregnancy. Hyperimmune globulin products such as VARIZIG and WinRho FDA-approved conditions pregnancy Varicella Zoster Rh-incompatibility. We administered ZIKV-specific human immune a treatment in pregnant rhesus macaques one day after subcutaneous ZIKV infection. All animals controlled viremia following the generated robust levels of anti-Zika antibodies their blood. No adverse fetal or infant outcomes were...
A possibly causal relationship between multiple sclerosis and chronic cerebrospinal venous insufficiency has recently been hypothesized. Studies investigating have reported conflicting results few employed diagnostic imaging modalities across a large patient control population. In this study, three complementary were used to investigate the hypothesis in patients with two age- sex-matched groups: healthy volunteers other neurological diseases. Strictly blinded Doppler ultrasound according...
Diagnostic tools for evaluating the supraorbital rim in preparation nerve decompression surgery patients with chronic headaches are currently limited. We evaluated use of sonography to diagnose presence a notch or foramen 11 cadaver orbits. Sonographic findings were assessed by dissecting orbits determine whether was present. Sonography correctly diagnosed 7 cases and 4 cases. found that had 100% sensitivity diagnosing foramen. This tool may therefore be helpful characterizing preoperatively...
Abstract Infection with Zika virus (ZIKV) is associated human congenital fetal anomalies. To model outcomes in nonhuman primates, we administered Asian-lineage ZIKV subcutaneously to four pregnant rhesus macaques. While non-pregnant animals a previous study contemporary the current report clear viremia within 10-12 days, maternal was prolonged 3 of 4 pregnancies. Fetal head growth velocity last month gestation determined by ultrasound assessment circumference decreased comparison biparietal...
The stiffness of the uterine cervix changes during pregnancy, significantly softening at term. A pathologically rapid can result in preterm birth. Shear Wave Elasticity Imaging (SWEI) methods be used to track these changes. Difficulties vivo human research pregnancy motivate study animal models with anatomy and physiology similar humans. This work presents preliminary results use SWEI assess cervical a rhesus macaque non-human primate (NHP) model. Pregnant NHP subjects are being scanned two...
Abstract Congenital Zika virus (ZIKV) infection impacts fetal development and pregnancy outcomes. We infected a pregnant rhesus macaque with Puerto Rican ZIKV isolate in the first trimester. The was complicated by preterm premature rupture of membranes (PPROM) demise 49 days post (gestational day 95). Significant pathology at maternal-fetal interface included acute chorioamnionitis, placental infarcts, leukocytoclastic vasculitis myometrial radial arteries. RNA disseminated throughout fetus...
We are investigating the use of shear wave elasticity imaging (SWEI) to remotely palpate cervix and track how it softens during pregnancy. This work presents preliminary results SWEI methods in a rhesus macaque nonhuman primate model that has similar anatomy physiology humans. Pregnant primates were scanned before mating, five times between weeks 4 23 after conception, two post-partum. was performed transabdominally with linear array transducer on clinical ultrasound system enabled provide...
Purpose: Quantitative ultrasound (QUS) provides acoustic parameters that are closely related to tissue microstructure and sound wave propagation. This study evaluated a Bayesian classifier applied breast QUS data aid in differentiating benign from malignant masses. presentation outlines methodology for deriving these quantitative reports preliminary results using the on small set of human tumors. Methods: IRB-approved, HIPAA compliant recruited 35 subjects who were scheduled core biopsy...
Abstract Background In utero Zika virus (ZIKV) infection causes birth defects and neurodevelopmental deficits in neonates. We reasoned that a translational macaque model of congenital ZIKV could define disease pathophysiology not possible human clinical studies. Methods inoculated 5 pregnant rhesus macaques with Puerto Rican isolate (ZIKV-PRVABC59) during the first trimester, monitored plasma viral RNA (vRNA) loads, evaluated infants for their week life. Assessments included neurobehavioral...
Abstract Congenital Zika virus (ZIKV) exposure results in a spectrum of disease ranging from severe birth defects to delayed onset neurodevelopmental deficits. ZIKV-related neuropathogenesis, predictors defects, and deficits are not well defined people. Here we assess the methodological statistical feasibility congenital ZIKV macaque model for identifying infant neurobehavior brain abnormalities that may underlie We inoculated five pregnant macaques with mock-inoculated one first trimester....