Summary Objective To verify the validity of measuring levels Mycobacterium leprae ‐specific anti‐phenolic glycolipid (PGL)‐I antibody, neopterin, a product activated macrophages, and C‐reactive protein (CRP), an acute phase protein, in serial serum samples from patients for monitoring leprosy spectrum reactions during course multi‐drug treatment (MDT). Methods Twenty‐five untreated patients, 15 multi‐bacillary (MB) 10 paucibacillary (PB), participated. Eight developed reversal reaction five...

Studies investigating the immunopathological aspects of Jorge Lobo's disease have shown that inflammatory infiltrate consists mainly histiocytes and multinucleated giant cells involving numerous yeast-like Lacazia loboi, with T lymphocytes more common than B plasma cells. The quantification cytokines in peripheral blood mononuclear culture supernatant has revealed alterations profile, characterized by predominance a Th2 profile. In view these findings role cell interactions, objective...

Erythema nodosum leprosum (ENL) is a frequent complication of multibacillary leprosy that can result in significant morbidity, including peripheral nerve damage and physical disability. The identification possible serum markers could be valuable tool for the early detection ENL.The purpose this study was to evaluate selected mediators involved innate adaptive immune responses identify immunomarkers levels interleukin-2, interleukin-4, interleukin-6, interleukin-10, interleukin-17,...

In order to attain a better understanding of the interactions between opportunist fungi and their hosts, we investigated cytokine profile associated with inflammatory response Candida albicans infection in mice solid Ehrlich tumors different degrees.Groups eight animals were inoculated intraperitoneally 5 x 106 C. 7, 14 or 21 days after tumor implantation. After 24 72 hours, euthanized intraperitoneal lavage fluid was collected. Peritoneal macrophages cultivated levels IFN-gamma, TNF-alpha,...

Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity hosts to mycobacterial infection. Studies on interaction monocyte-derived DCs (MO-DCs) using Mycobacterium leprae leprosy patients are rare. The present study demonstrated that differentiation MOs was similar all forms compared normal healthy individuals. In vitro stimulation immature MO-DCs with sonicated M. induced variable degrees DC maturation as determined by increased expression HLA-DR, CD40,...

Since there are no studies evaluating the participation of complement system (CS) in Jorge Lobo's disease and its activity on fungus Lacazia loboi, we carried out present investigation. Fungal cells with a viability index 48% were obtained from footpads BALB/c mice incubated pool inactivated serum patients mycosis or sterile saline for 30 min at 37 masculineC. Next, tubes 2 h noninactivated AB+ serum, diluted EGTA-MgCl2, EDTA. The L. loboi was evaluated fungal suspension cytocentrifuged....

Sixty-four isogenic Swiss mice were intradermically inoculated in both hind foot pads. The inocula, consisting of fungal suspensions from biopsies obtained Jorge Lobo's Disease patients, had the total number fungi and viability index determined using a Neubauer chamber fluorescein diacetate-ethidium bromide technique (FD-EB), respectively. animals sacrificed at times ranging ten days to eighteen months after inoculation. cellular infiltrate, mainly macrophages containing fungi, increased...

Thalidomide is a selective inhibitor of tumor necrosis factor-alpha (TNF-alpha), cytokine involved in mycobacterial death mechanisms. We investigated the role this drug functional activity alveolar macrophages presence infection induced by intranasal inoculation Mycobacterium avium thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 10(6) M. respiratory route. Thirty received daily thalidomide (30 mg/kg mouse) 30 water gavage up to sacrifice. Ten...

Studies on host-parasite interaction in Jorge Lobo's disease are scarce, with no report the literature phagocytosis of Lacazia loboi by phagocytic mononuclear cells. Thus, objective present study was to assess activity blood monocytes presence L. patients and healthy subjects (controls) over 3 24 hours incubation. Statistical analyses results showed significant difference percent fungus between patient control monocytes. With respect incubation time, however, there a difference, that higher...

The viability of the currently unculturable fungal pathogen Lacazia loboi can be determined by means fluorescein diacetate-ethidium bromide (FD-EB) staining. This technique used in experimental study mycosis, attempts to cultivate fungus and gauge success therapies. In present study, potential applications FD-EB vital staining were studied using a proposed murine model lobomycosis. BALB/c mice inoculated footpads with an L. suspension that appeared have lost after being held for 15 days at...

To better understand the interactions between opportunistic fungi and their hosts, we investigated hydrogen peroxide (H2O2), nitric oxide TNF-alpha production by peritoneal macrophages from Ehrlich tumour-bearing mice (TBM) during microbial infections. For this purpose, TBM at days 7, 14 21 of tumour progression were inoculated intraperitoneally with C. albicans evaluated after 24 72 h. We observed that showed significant increases in H2O2, levels fungal clearance day 7 infection. However,...

Summary This study evaluated the immune response of nude and BALB /c mice inoculated in footpads ( FP ) with Mycobacterium leprae after 3, 5 8 months. At each timepoint peritoneal cells, peripheral blood, popliteal lymph nodes PLN were collected. Peritoneal cell cultures performed to measure H 2 O , − NO IL ‐2, ‐4, ‐10, ‐12, IFN ‐γ TNF levels. Serum levels anti‐ PGL ‐I antibodies also quantified. The results showed that infection was progressive bacterial multiplication, development...

SUMMARY The aim of this study was to evaluate the effects protein-calorie malnutrition in BALB/c isogenic mice infected with Lacazia loboi, employing nutritional and histopathological parameters. Four groups were composed: G1: inoculated restricted diet, G2: not G3: regular G4: diet. Once had been imposed, animals intradermally footpad after four months, sacrificed for excision footpad, liver spleen. infection did exert great influence on body weight mice. spleen showed reduction...

The authors investigated the relationship between dermatophytosis and ABO blood groups through typing, identification of isolated dermatophytes specific cellular immune response 40 individuals carriers this mycosis. They verified that fungus Trichophyton rubrum, from 54.5% patients, was more frequent in belonging to group A. response, evaluated trichophytin antigen, positive 25% studied patients; presence immediate reactions (30 minutes) 35%. distribution among patients with control was,...

Introdução: a hanseníase é uma doença in fecciosa crônica causada pelo Mycobacterium leprae (M. leprae), um parasita intracelular obrigatório. Assim, resistência do hospedeiro esse patógeno depende da imunidade celular. O uso de modelos experimentais tem permitido o estudo ponto vista imunológico, microbiológico e terapêutico, entretanto, as diferenças na progressão infecção entre os mais empregados (camundongos imunocompetentes, BALB/c, camundongos congenitamente atímicos, nude) são pouco...

There are no studies investigating the role of nutritional status and immunity associated with Jorge Lobo's disease. The objective this study was to evaluate effects protein-calorie malnutrition on immune response BALB/c mice inoculated Lacazia loboi. In study,the animals were divided into four groups: G1: restricted diet, G2: not G3: regular G4: diet. groups G1 G2 submitted for 20 days once installed intradermally footpad. After 4 months, they euthanised isolation peritoneal lavage cells...

The murine model of Jorge Lobo's disease is characterized by histological alterations similar to those seen in human disease, including a large number viable fungi. This study evaluated the immune response mice with early and late macroscopic lesions (5 13 months post-inoculation [p.i.], respectively) analysis peritoneal lavage cells footpad (FP) histology. FP were inoculated 1 × 106 fungi (viability index 41%). At 5 p.i., granuloma mainly consisted macrophages multinucleated giant cells,...

Jorge Lobo's disease, also known as lacaziosis, is a cutaneous-subcutaneous mycosis with chronic evolution. It caused by the fungus Lacazia loboi. Herein we report study that relates genotoxicity L. loboi in isogenic mice nutritional status, through normal or restricted diet.DNA damage was assessed peripheral blood comet assay (tail intensity).The results for leukocytes showed increases mean tail intensity under dietary restriction, infected restriction and ingesting diet.These indicate...