A5012729676- Extracellular vesicles in disease
- Fermentation and Sensory Analysis
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Bacteriophages and microbial interactions
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Horticultural and Viticultural Research
- Microbial Inactivation Methods
- Mercury impact and mitigation studies
- Plant Molecular Biology Research
- Alzheimer's disease research and treatments
- Microbial Metabolites in Food Biotechnology
- Neural dynamics and brain function
- Cellular transport and secretion
- Heavy metals in environment
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Radioactive element chemistry and processing
- Food Drying and Modeling
- Microbial Metabolism and Applications
Centre National de la Recherche Scientifique
2015-2025
Inserm
2014-2025
Université de Bordeaux
2015-2025
Imaging Center
2020-2024
Bordeaux Imaging Center
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Bordeaux Population Health
2014-2023
Pulsed electric field (PEF) treatment is an emerging technology that arousing increasing interest in vinification processes for its ability to enhance polyphenol extraction performance. The aim of this study was investigate the effects PEF on grape skin histocytological structures and organization cell wall polysaccharides tannins, which, until now, have been little investigated. This relates two treatments harvested Cabernet Sauvignon berries: PEF1 (medium strength (4 kV/cm); short duration...
Abstract Dopamine transmission is involved in reward processing and motor control, its impairment plays a central role numerous neurological disorders. Despite strong pathophysiological relevance, the molecular structural organization of dopaminergic synapse remains to be established. Here, we used targeted labelling fluorescence activated sorting purify striatal synaptosomes. We provide proteome synapses with 57 proteins specifically enriched. Beyond canonical markers dopamine...
Abstract In Alzheimer's disease (AD), the distribution of amyloid precursor protein (APP) and its fragments other than beta, has not been fully characterized. Here, we investigate APP in human AD brain samples mouse models reference to proteases, synaptic proteins, histopathological features characteristic brain, by combining an extensive set histological analytical tools. We report that prominent somatic observed control patients remarkably vanishes benefit dense accumulations extra‐somatic...
Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences O. (14 already available and 36 newly sequenced ones) provided an inventory genes potentially involved exopolysaccharide (EPS) biosynthesis. loci identified are: two gene clusters named eps1 eps2, three isolated glycoside-hydrolase dsrO, dsrV levO, glycosyltransferase gtf, it3, it4. were present or absent depending on strain eps composition diverged from one to another....
The Gluconobacter phage GC1 is a novel member of the Tectiviridae family isolated from juice sample collected during dry white wine making. bacteriophage infects cerinus, an acetic acid bacterium which represents spoilage microorganism making, mainly because it able to produce ethyl alcohol and transform into acid. Transmission electron microscopy revealed tail-less icosahedral particles with diameter ~78 nm. linear double-stranded DNA genome (16,523 base pairs) contains terminal inverted...
Diverging from conventional cell division models, plant cells undergo incomplete to generate plasmodesmata communication bridges between daughter cells. Although fundamental for multicellularity, the molecular events leading bridge stabilization, as opposed severing, remain unknown. Using electron tomography, we mapped transition plate fenestrae plasmodesmata. We show that endoplasmic reticulum (ER) connects across fenestrae, and matures, contract, causing plasma membrane (PM) mold around...
Methylmercury (MeHg) is highly toxic and mainly produced in anoxic environments by certain microorganisms. Net MeHg production involves a series of separate cellular processes: the uptake inorganic divalent Hg (Hg(II)) cell, intracellular enzymatic Hg(II) methylation, release into extracellular medium, as well demethylation. As biological process, saturation at level can be anticipated all stages transformation. The aim this study was to investigate kinetics methylation demethylation over...
Immune checkpoint inhibitors have produced encouraging results in cancer patients. However, the majority of ß-catenin-mutated tumors been described as lacking immune infiltrates and resistant to immunotherapy. The mechanisms by which oncogenic ß-catenin affects surveillance remain unclear. Herein, we highlighted involvement regulation exosomal pathway and, extension, immune/cancer cell communication hepatocellular carcinoma (HCC). We showed that mutated represses expression SDC4 RAB27A , two...
Microorganisms are key players in the transformation of mercury into neurotoxic methylmercury (MeHg). Nevertheless, this mechanism and opposite MeHg demethylation remain poorly understood. Here, we explored impact inorganic (IHg) concentrations from 0.05 to 50 µM on production degradation two sulfate-reducing bacteria, Pseudodesulfovibrio hydrargyri BerOc1 able methylate demethylate Desulfovibrio desulfuricans G200 only MeHg. produced by increased with increasing IHg concentration a maximum...
Abstract Background Targeting G protein-coupled receptors on the surface of cancer cells with peptide ligands is a promising concept for selective tumor delivery therapeutically active cargos, including radiometals targeted radionuclide therapy (TRT). Recently, radiolanthanide terbium-161 ( 161 Tb) gained significant interest TRT application, since it decays medium-energy β-radiation but also emits amount conversion and Auger electrons short tissue penetration range. The therapeutic...
Diverging from conventional cell division models, plant cells undergo incomplete to generate plasmodesmata communication bridges between daughter cells. While fundamental for multicellularity, the molecular events leading bridge stabilization, as opposed severing, remain unknown. Using electron tomography, we mapped transition plate fenestrae plasmodesmata. We show that ER connects across fenestrae, and matures, contract, causing PM mold around constricted tubes. The ER's presence prevents...
Oenococcus oeni is the lactic acid bacteria species which most adapted to wine. Recently, two groups of strains that form genetic lineages were described in red and white Burgundy wines. The aim this study was analyse phenotypes these order determine how they have specifically either or wine.Four from each group tested grape must wines evaluate their tolerance pH phenolic compound content. White wine proved be tolerant low pH, both wine, whereas inhibited by presence tannins Red more...
To provide insights into phage-host interactions during winemaking, we assessed whether phenolic compounds modulate the phage predation of Oenococcus oeni. Centrifugal partition chromatography was used to fractionate a model red wine. The ability lytic oenophage OE33PA kill its host reduced in presence two collected fractions which identified five compounds. Three, namely, quercetin, myricetin and p-coumaric acid, significantly O. oeni when provided as individual pure molecules, also did...
Diffuse midline glioma (DMG) is a pediatric malignancy with poor prognosis. Most children die less than one year after diagnosis. Recently, mutations in histone H3 have been identified and are believed to be oncogenic drivers. Targeting this epigenetic abnormality using deacetylase (HDAC) inhibitors such as panobinostat (PS) therefore novel therapeutic option currently evaluated clinical trials.BH3 profiling revealed engagement an irreversible apoptotic process of cells exposed PS confirmed...
Immune checkpoint inhibitors have produced encouraging results in cancer patients. However, the majority of ß-catenin-mutated tumors been described as lacking immune infiltrates and resistant to immunotherapy. The mechanisms by which oncogenic ß-catenin affects surveillance remain unclear. Herein, we highlighted involvement regulation exosomal pathway and, extension, immune/cancer cell communication hepatocellular carcinoma (HCC). We showed that mutated represses expression SDC4 RAB27A , two...
The aim of this research was to investigate the efficiency microwaves (MW) for wine microbiological stabilization and thereby assess potential application a Weibullian mathematical model in industry. study focused on (i) influence treatment time (come-up temperature maintenance) simple wine-like matrix, as well (ii) specific resistance yeast bacteria selected according their representativeness red wines (A. aceti ATCC_15973, S. cerevisiae FX10, B. bruxellensis CRBO_L0619 AWRI_1499), finally...
SUMMARY Dopamine is a monoamine involved in reward processing and motor control. Volume transmission thought to be the mechanism by which monoamines modulate effector systems at glutamate GABA synapses. Hence, dopamine synapses are scarcely described. We applied fluorescence activated synaptosome sorting explore features of dopaminergic synaptome. provide proteome striatal with 57 proteins specifically enriched. Beyond canonical markers neurotransmission (Th, Slc6a3/DAT, Slc18a2/VMAT2), we...
This work investigated the structural, biochemical, and molecular characteristics of grape skin cell wall during ripening, related to susceptibility Botrytis cinerea. The comparative study between two main cultivars in Champagne region, Pinot noir Chardonnay, quantified: (1) maturity physical profile skin; (2) morphological characteristics; (3) soluble pectic polysaccharides located walls; (4) gene expression degrading enzymes (VvPME1 VvPG1) PME activity. During maturation period, skins...
Immune checkpoint inhibitors have produced encouraging results in cancer patients. However, the majority of ß-catenin mutated tumors been described as lacking immune infiltrates and resistant to immunotherapy. The mechanisms by which oncogenic affects surveillance remain unclear. Herein, we highlighted involvement regulation exosomal pathway immune/cancer cell communication hepatocellular carcinoma (HCC). We showed that represses SDC4 RAB27A expression, two main actors exosome biogenesis,...
Immune checkpoint inhibitors have produced encouraging results in cancer patients. However, the majority of β-catenin-mutated tumors been described as lacking immune infiltrates and resistant to immunotherapy. The mechanisms by which oncogenic β-catenin affects surveillance remain unclear. Herein, we highlighted involvement regulation exosomal pathway and, extension, immune/cancer cell communication hepatocellular carcinoma (HCC). We showed that mutated represses expression SDC4 RAB27A , two...