A5059642606- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Urban Stormwater Management Solutions
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Cancer-related Molecular Pathways
- Wastewater Treatment and Reuse
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Recycling and Waste Management Techniques
- Smart Materials for Construction
- RNA Interference and Gene Delivery
- Cancer Treatment and Pharmacology
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Bone Tissue Engineering Materials
- Advanced biosensing and bioanalysis techniques
- Microplastics and Plastic Pollution
Cancer Research Institute Ghent
2021-2025
Ghent University
2014-2025
Ghent University Hospital
2023-2025
Abstract Spheroids are three-dimensional cellular models with widespread basic and translational application across academia industry. However, methodological transparency guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation this identified heterogeneity in setup spheroids. Empirical evaluation interlaboratory...
The application of three-dimensional (3D) cell cultures such as spheroids and organoids is growing in popularity both academia industry. However, morphology the 3D architecture remains remarkably understudied. Here, we introduce an open-access Spheroid Light Microscopy Image Atlas (SLiMIA) that can serve a training set for studies cultures. We provide images with variety metadata: 9 microscopes, 47 lines, 8 culture media, 4 spheroid formation methods multiple seeding densities; totalling...
Spheroids are near-spherical reaggregated multicellular three-dimensional (3D) structures generated from cells or cell cultures healthy as well pathological tissue. Basic and translational spheroid application across academia industry led to the development of multiple set-ups analysis methods which mostly lack modularity maximally phenotype spheroids. This protocol presents self-assembly single-cell suspensions into spheroids by liquid overlay method followed a modular framework for...
Taxanes, such as paclitaxel (PTX), stabilize microtubules and are used a first-line therapy in multiple cancer types. Disruption of microtubule equilibrium, which plays an essential role mitosis cell homeostasis, ultimately results death. Even though PTX is very potent chemotherapy, its use associated with major side effects resistance. Pelophen B (PPH), synthetic analog peloruside A, stabilizes through interaction non-taxoid binding site β-tubulin. We evaluated the anticancer effect PPH...
Abstract Background Long-term drug evaluation heavily relies upon rodent models. Drug discovery methods to reduce animal models in oncology may include three-dimensional (3D) cellular systems that take into account tumor microenvironment (TME) cell types and biomechanical properties. Methods In this study we reconstructed a 3D using an elastic polymer (acrylate-endcapped urethane-based poly(ethylene glycol) (AUPPEG)) with clinical relevant stiffness. Single suspensions from low-grade serous...
In vitro cell culture experiments are widely used to study cellular behavior in most biological research fields. Except for suspension cells, human types cultured as adherent monolayers on a plastic surface. While technically convenient, monolayer cultures can suffer from limitations terms of physiological relevance, their resemblance complex vivo tissue structures is limited. To address these limitations, three-dimensional (3D) systems have gained increased interest they mimic key...
Scrap material recovery and recycling companies are producing wastewater in which common pollutants (such as COD, nutrients suspended solids), toxic metals, polyaromatic hydrocarbons (PAH) polychlorinated biphenyls (PCB) frequently can exceed the discharge limits. Lab-scale optimisation of different possible physical–chemical treatment techniques was performed on originating from three view further testing at pilot-scale implementation full-scale. The lab-scale tests demonstrate that...