A5086124798- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Intraperitoneal and Appendiceal Malignancies
- Medical Imaging Techniques and Applications
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- Colorectal Cancer Surgical Treatments
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Cancer, Stress, Anesthesia, and Immune Response
- Radiomics and Machine Learning in Medical Imaging
- Nanoparticle-Based Drug Delivery
- Cardiac Ischemia and Reperfusion
- Adipose Tissue and Metabolism
- Proteoglycans and glycosaminoglycans research
- Lung Cancer Research Studies
- Additive Manufacturing and 3D Printing Technologies
- Mesenchymal stem cell research
- Esophageal Cancer Research and Treatment
- TGF-β signaling in diseases
- Hydrogels: synthesis, properties, applications
KU Leuven
2025
Ghent University
2013-2024
Cancer Research Institute Ghent
2013-2023
Ghent University Hospital
2014-2023
Mesenchymal stromal cells (MSCs) hold significant potential for various applications in regenerative medicine and tissue engineering. Initially considered as a single cell type with defined characteristics, MSCs are now known heterogeneous population remarkable differences their properties. No consensus exists on how donor age affects MSC like proliferation. Additionally, differentiation capacities immunophenotype could arise when isolated from different animals breeds, which is relevant...
Abstract Preoperative radiotherapy (RT) is a mainstay in the management of rectal cancer, tumor characterized by desmoplastic stroma containing cancer-associated fibroblasts (CAF). Although CAFs are abundantly present, effects RT to CAF and its impact on cancer cells unknown. We evaluated damage responses investigated changes colorectal cell growth, transcriptome, metabolome, kinome response paracrine signals emerging from irradiated CAF. induced DNA damage, p53 activation, cell-cycle...
Abstract Spheroids are three-dimensional cellular models with widespread basic and translational application across academia industry. However, methodological transparency guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation this identified heterogeneity in setup spheroids. Empirical evaluation interlaboratory...
Abstract Current thoroughly described biodegradable and cross‐linkable polymers mainly rely on acrylate cross‐linking. However, despite the swift cross‐linking kinetics of acrylates, concomitant brittleness resulting materials limits their applicability. Here, photo‐cross‐linkable poly(ε‐caprolactone) networks through orthogonal thiol‐ene chemistry are introduced. The step‐growth polymerized tunable, predictable by means rubber elasticity theory it is shown that mechanical properties...
Cancer progression is characterized by a complex reciprocity between neoplastic epithelium and adjacent stromal cells.In ductal carcinoma in situ (DCIS) of the breast, both reduced decorin expression myxoid stroma are correlated with increased recurrence risk.In this study, we aimed to investigate paracrine regulation related extracellular matrix (ECM) proteins cancerassociated fibroblasts (CAFs).Transforming growth factor-β1 (TGF-β1) was identified as competent ECM modulator, it strongly...
Here we report that grafting temperature-responsive polymers onto hyaluronic acid allows temperature-induced self-assembly into nanogels with tunable size. These can be easily loaded hydrophobic molecules and hold potential for anti-cancer drug delivery towards human ovarian cancer cells.
Esophageal cancer is an aggressive disease with poor survival rates. A more patient-tailored approach based on predictive biomarkers could improve outcome. We aimed to predict radiotherapy (RT) response by imaging tumor hypoxia 18F-FAZA PET/CT in esophageal adenocarcinoma (EAC) mouse model. Additionally, we investigated the radiosensitizing effect of modifier nimorazole vitro and vivo.In MTS cell proliferation assays (OACM5 1.C SC1, human EAC line) were performed under normoxic hypoxic (<...
This data paper contains information about the in vivo model for peritoneal implants used "Tumor-environment biomimetics delay metastasis formation by deceiving and redirecting disseminated cancer cells" (De Vlieghere et al., 2015) [1]. A double selection of SK-OV-3 Luc human ovarian cell line was to create IP1 IP2 lines. shows functional activities three lines vitro vivo. Phase-contrast images show morphology these cells, metabolic luciferase activity has been determined. Survival mice...
For the development of effective anti‐cancer vaccines, tumor associated antigens need to be internalized by antigen presenting cells alongside specific co‐stimulatory signals. Interestingly, relative soluble antigens, nano‐ and micro‐particulate are much better presented CD8 T cells, a crucial step in induction cytotoxic that can eliminate malignant cells. In this regard, generic strategy encapsulate cancer cell derived proteins into particulate delivery system would high interest. Here we...
Ovarian cancer carries a significant mortality. Since symptoms tend to be minimal, the disease is often diagnosed when peritoneal metastases are already present. The standard of care in advanced ovarian consists platinum-based chemotherapy combined with cytoreductive surgery. Unfortunately, even after optimal cytoreduction and adjuvant chemotherapy, most patients stage III will develop recurrence. Intraperitoneal administration an alternative treatment for localized disease. pharmacological...
There is a high demand for in vitro intestinal models towards studying disfunctions, such as gastrointestinal diseases (Crohn's disease, irritable bowel syndrome, colorectal cancer, etc.) which are commonly diagnosed modern society. In parallel, utilized the evaluation of novel food supplements and pharmaceuticals micro-environment. Gelatin-based hydrogels can offer appropriate mechanical cues (down to G' < 10 kPa) cell interactivity obtain physiologically relevant soft tissue models....
Abstract Background Long-term drug evaluation heavily relies upon rodent models. Drug discovery methods to reduce animal models in oncology may include three-dimensional (3D) cellular systems that take into account tumor microenvironment (TME) cell types and biomechanical properties. Methods In this study we reconstructed a 3D using an elastic polymer (acrylate-endcapped urethane-based poly(ethylene glycol) (AUPPEG)) with clinical relevant stiffness. Single suspensions from low-grade serous...
Aims: Regeneration in skeletal muscle relies on regulated myoblast migration and differentiation, which the transcription factor nuclear of activated T-cells 5 (NFAT5) participates. Impaired regeneration chronic inflammation are prevalent myositis. Little is known about impact NFAT5 localization expression this group diseases. The goal study was to investigate physiology unaffected myoblasts exposed cytokine or hyperosmolar stress Methods: intracellular were studied vitro using a cell...
A new analytical method for quantitative high-resolution LA-ICP-MS/MS imaging of FeO<sub>x</sub> NPs in gelatin microspheres.
// Alfiah Noor 1 , Ijeoma Adaku Umelo Peter Kronenberger 1, 2 Philippe Giron Elly De Vlieghere 3 Olivier Wever Erik Teugels and Jacques Grève Laboratory of Molecular Oncology Department Medical Oncology, Oncologisch Centrum, Universitair Ziekenhuis Brussel, Brussels, Belgium Biotechnology, Healthcare, Erasmushogeschool Experimental Cancer Research, Research Institute Ghent (CRIG), University, Ghent, Correspondence to: Grève, email: Jacques.Degreve@uzbrussel.be Noor, alfinoor@vub.ac.be...
// Joke Tommelein 1, 2 , Félix Gremonprez 2, 3 Laurine Verset 4 Elly De Vlieghere Glenn Wagemans Christian Gespach 5 Tom Boterberg Pieter Demetter Wim Ceelen Marc Bracke Olivier Wever 1 Laboratory of Experimental Cancer Research, Department Radiation Oncology and Ghent University, Ghent, Belgium Research Institute (CRIG), Surgery, University Hospital, Pathology, Erasme Université Libre de Bruxelles, Brussels, Institut National la Santé et Recherche Médicale, INSERM, Molecular Clinical...
FHL2 is a multifunctional scaffolding protein; its expression associated with poor prognosis in colorectal cancer. ADAM-17 metalloprotease implicated ectodomain shedding. regulates plasma membrane localisation, and deficiency leads to decreased activity of mouse macrophages. Presence relationship the ADAM-17/FHL2 complex cancer progression unknown. We studied several colon cell lines by immunocytochemistry western blot. To highlight interaction between both molecules, we used Duolink® kit...