A5066331381- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Reproductive Health and Technologies
- Piezoelectric Actuators and Control
- Micro and Nano Robotics
- AI in cancer detection
- Image Processing Techniques and Applications
- Force Microscopy Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Near-Field Optical Microscopy
- Soft Robotics and Applications
- Mechanical and Optical Resonators
- Additive Manufacturing and 3D Printing Technologies
- Cellular Mechanics and Interactions
- Immune cells in cancer
- Assisted Reproductive Technology and Twin Pregnancy
- MicroRNA in disease regulation
- Orbital Angular Momentum in Optics
- Advanced Neural Network Applications
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Microfluidic and Capillary Electrophoresis Applications
University of Toronto
1995-2025
Dalian University of Technology
2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2024
Sun Yat-sen University
2024
University of New Brunswick
2022
Beihang University
2013-2018
Ministry of Education of the People's Republic of China
2016
Inspired by the collective intelligence in natural swarms, microrobotic agents have been controlled to form artificial swarms for targeted drug delivery, enhanced imaging, and hyperthermia. Different from these well-investigated tasks, this work aims develop embolization, which is a clinical technique used block blood vessels treating tumors, fistulas, arteriovenous malformations. Magnetic particle were formed selective embolization address low selectivity of present that prone cause...
Robotic manipulation of deformable objects has been a classic topic in robotics. Compared to synthetic such as rubber balls and clothes, biological cells are highly more prone damage. This article presents robotic for orientation control (both out-of-plane in-plane), which is required both clinical (e.g., vitro fertilization) biomedical clone) applications. manual cell based on empirical experience, the approach, modeling path planning, effectively rotates cell, while consistently...
Heart beating is triggered by the generation and propagation of action potentials through myocardium, resulting in synchronous contraction cardiomyocytes. This process highlights importance electrical mechanical coordination organ function. Investigating pathogenesis heart diseases potential therapeutic actions vitro requires biosensing technologies which allow for long-term simultaneous measurement contractility electrophysiology However, adoption current approaches functional cardiac...
In infertility treatment, blastocyst morphological grading is commonly used in clinical practice for evaluation and selection, but has shown limited predictive power on live birth outcomes of blastocysts. To improve prediction, a number artificial intelligence (AI) models have been established. Most existing AI only images the area under receiver operating characteristic (ROC) curve (AUC) achieved by these plateaued at ~0.65.This study proposed multimodal method using both patient couple's...
Emerging heart-on-a-chip technology is a promising tool to establish in vitro cardiac models for therapeutic testing and disease modeling. However, due the technical complexity of integrating cell culture chambers, biosensors, bioreactors into single entity, microphysiological system capable reproducing controlled microenvironmental cues regulate phenotypes, promote iPS-cardiomyocyte maturity, simultaneously measure dynamic changes cardiomyocyte function situ not available. This paper...
In cell biology, fluorescent dyes are routinely used for biochemical measurements. The traditional global dye treatment method suffers from low signal-to-noise ratios (SNR), especially when detecting a concentration of ions, and increasing the causes more severe cytotoxicity. Here, we report robotic technique that controls how amount fluorescent-dye-coated magnetic nanoparticles accurately forms swarm increases in local region inside intracellular measurement. Different existing...
Recent interest in high-precision positioning stages with large travel ranges has sparked renewed attention to the development of voice coil motors (VCMs). Due their output force, VCMs can actuate more complicated flexure structures, eliminate rail friction, and improve speed. The VCM structure is both compact flexible; hence, it convenient design for a variety stage structures. Furthermore, combined other actuators are able achieve high precision. In this paper, we summarize principles...
Can a quantitative method be developed to differentiate between blastocysts with similar or same inner cell mass (ICM) and trophectoderm (TE) grades, while also reflecting their potential for live birth? We BlastScoringNet, an interpretable deep-learning model that quantifies blastocyst ICM TE morphology continuous scores, enabling finer differentiation higher scores significantly correlating birth rates. While the Gardner grading system is widely used by embryologists worldwide, having...
The robotic manipulation of deformable linear objects is a classic and challenging topic. Apart from synthetic objects, such as wires cables, are also commonly found in biological cells organisms. Biomanipulation hampered by difficulties, limited degrees freedom micromanipulators varied mechanical properties the entities to manipulate. This article presents human sperm, which with shape. shape movement cell recapitulated our developed geometric kinematic models. Under unfixed constraints...
MRNA vaccines have been shown to provide strong immune responses against SARS-CoV-2, but the extent of their antibody cross-reactivity human seasonal coronaviruses, such as NL63, remains unclear. Furthermore, it is unknown whether pre-existing NL63 might affect outcome SARS-CoV-2 mRNA vaccination. This study used a flow cytometry-based serological assay and an in vitro neutralization analyze sera from mRNA-vaccinated mice plasma samples vaccinated cohort. We found that Moderna mRNA-1273...
Cell penetration is an indispensable step in many cell surgery tasks. Conventionally, achieved by passively indenting and eventually puncturing the membrane, during which undesired large deformation induced. Piezo drills have been developed to penetrate cells with less deformation. However, existing piezo suffer from lateral vibration or are incompatible standard clinical setup. Furthermore, it challenging accurately determine time instance of membrane puncturing; thus, delay stop pulsing...
Proper alignment of the end-effector is a critical procedure that determines success micromanipulation, such as robotic cell manipulation. Presently, performed manually and suffers from large misalignment error inconsistency. Manual often undesirably moves (e.g., glass micropipette) out limited field view under microscopy risks breaking fragile end-effector. This article presents automated in micromanipulation. A rotational degree freedom was added to standard micromanipulator with...
The nuclear envelope (NE) separates genomic DNA from the cytoplasm and regulates transport between cytosol nucleus in eukaryotes. Nuclear stiffening enables cell to protect itself extensive deformation, loss of NE integrity, genome instability. It is known that reorganization actin, lamin, chromatin can contribute stiffening. In this work, we show structural alteration also contributes instantaneous under indentation. situ mechanical characterization nuclei intact cells shows unfolding...
Precise rotational positioning of end-effectors under microscopy is crucial for robotic micromanipulation. However, the end-effector presently limited to a fixed orientation, which manually set before given micromanipulation task, lacking accuracy and versatility <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in situ</i> reorientation. In this article, we present unified framework rotationally in three dimensions by establishing general...
Aspirating a single cell from the outside to inside of micropipette is widely used for transfer and manipulation. Due small volume (picoliter) nonlinear dynamics involved in aspiration process, it challenging accurately quickly position target micropipette. This letter reports first mathematical model that describes motion micropipette, which takes into account oil compressibility connecting tube's deformation. Based on model, an adaptive controller was designed effectively compensate error...
Abstract Background Deep learning has been increasingly investigated for assisting clinical in vitro fertilization (IVF). The first technical step many tasks is to visually detect and locate sperm, oocytes, embryos images. For deployment of such deep models, different clinics use image acquisition hardware sample preprocessing protocols, raising the concern over whether reported accuracy a model by one clinic could be reproduced another clinic. Here we aim investigate effect each imaging...
Atomic force microscopes are a key tool in nanotechnology that overcome the limitations of optical and provide imaging capabilities with nanoscale resolution. We have developed an atomic microscope uses inexpensive quartz tuning fork as micro cantilever. Because its ease operation open structure, it can be easily customized by students. Due to low costs, is possible every student course has access one setup, allowing all students obtain deep insights into understand principles microscopy.