A5081433169- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- CAR-T cell therapy research
- Estrogen and related hormone effects
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Developmental Biology and Gene Regulation
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- PI3K/AKT/mTOR signaling in cancer
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- S100 Proteins and Annexins
- Ferroptosis and cancer prognosis
- Advanced Breast Cancer Therapies
- Chronic Lymphocytic Leukemia Research
- Galectins and Cancer Biology
- Cancer-related molecular mechanisms research
- Cytokine Signaling Pathways and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Thyroid Cancer Diagnosis and Treatment
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
Hospital for Sick Children
2015-2025
University of Toronto
2015-2025
SickKids Foundation
2002-2024
Great Ormond Street Hospital
2013-2024
University College London
1994-2024
Biologie du Développement et Cellules Souches
2009-2015
University Health Network
2011-2014
Ontario Institute for Cancer Research
2014
Division of Undergraduate Education
2013
Princess Margaret Cancer Centre
2012
Aberrant activation of Notch receptors has been shown to cause mammary tumors in mice. We therefore used situ hybridization analyze expression ligands and human breast cancer. High levels JAG1 NOTCH1 were noted a subset with poor prognosis pathologic features (P < 0.05). tissue microarrays the these genes collection cancers from patients representing wide spectrum clinical stages, whom associated follow-up survival data was available (n = 184). Patients expressing high or had significantly...
Actin-binding protein (ABP-280, nonmuscle filamin) is a ubiquitous dimeric actin cross-linking phosphoprotein of peripheral cytoplasm, where it promotes orthogonal branching filaments and links to membrane glycoproteins. The complete nucleotide sequence human endothelial cell ABP cDNA predicts polypeptide subunit chain 2,647 amino acids, corresponding 280 kD, also the mass derived from physical measurements native protein. actin-binding domain near amino-terminus acid similar other filament...
Abstract Background Human breast cancer is a heterogeneous disease consisting of multiple molecular subtypes. Genetically engineered mouse models are useful resource for studying mammary cancers in vivo under genetically controlled and immune competent conditions. Identifying murine with conserved human tumor features will facilitate etiology determinations, highlight the effects mutations on pathway activation, should improve preclinical drug testing. Results Transcriptomic profiles 27...
T lymphocytes contain both Grb2, an SH2 and SH3 domain containing adaptor protein, Sos, a guanine nucleotide exchange factor for Ras. Immunoprecipitates of Sos from the lysates cells 36-kDa protein which is phosphorylated on tyrosine residues in response to cell receptor/CD3 cross-linking. In vitro studies using different bacterially synthesized GST-Sos fusion proteins confirm formation complexes p36 proline-rich COOH-terminal Sos. The use mutant GST-Grb2 domains have been mutationally...
Using three independent approaches, we studied the effects of H-ras on metastasis formation. Analysis five in vitro-ras-transfected 10T1/2 clones with either flat or refractile morphologies revealed a relationship between metastatic potential, expression, and anchorage-independent growth. Four variants derived from poorly metastatic, low-H-ras-expressing line all expressed high levels RNA grew efficiently soft agar. Activation expression tumors had occurred through amplification...
Abstract PIK3CA, which codes for the p110α catalytic subunit of phosphatidylinositol 3-kinase, is one most frequently mutated genes in human breast cancer. Here, we describe a mouse model PIK3CA-induced cancer by using ROSA26 (R26) knock-in system, targeted Pik3ca alleles can be activated through transgenic expression Cre recombinase. We mated Pik3caH1047R and Pik3cawt lines with MMTV-Cre transgenics, express mammary epithelium. Starting at approximately 5 months age, female...
Triple-negative breast cancer (TNBC) includes basal-like and claudin-low subtypes for which no specific treatment is currently available. Although the retinoblastoma tumor-suppressor gene (RB1) frequently lost together with TP53 in TNBC, it not directly targetable. There thus great interest identifying vulnerabilities downstream of RB1 that can be therapeutically exploited. Here, we determined combined inactivation murine Rb p53 diverse mammary epithelial cells induced claudin-low–like TNBC...
Oncogenes encoding serine/threonine or tyrosine kinases were introduced into the established rodent fibroblast cell line NIH 3T3 and tested for tumorigenic metastatic behavior in T cell-deficient nude mice. Transforming oncogenes of ras family capable converting lines to fully tumors. Cell transformed by kinase mos , ra f, src fes fms formed experimental metastases (in some cases) these genes more efficient at conversion than a mutant gene. In contrast, cells either two nuclear oncogenes,...
Triple negative breast cancer (TNBC) includes basal-like and claudin-low subtypes for which only chemotherapy radiation therapy are currently available. The retinoblastoma (RB1) tumor suppressor is frequently lost in human TNBC. Knockdown of RB1 luminal BC cells was shown to affect response endocrine, several antineoplastic drugs. However, the effect status on chemo-sensitivity TNBC whether affects divergent or specific treatment unknown. Using multiple cell lines, we hereby demonstrate that...