A5012581113- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Salmonella and Campylobacter epidemiology
- Protein Structure and Dynamics
- Ion Channels and Receptors
- ATP Synthase and ATPases Research
- Microbial Metabolic Engineering and Bioproduction
- Neuroscience and Neuropharmacology Research
- Bacteriophages and microbial interactions
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Ion channel regulation and function
- RNA regulation and disease
- Vibrio bacteria research studies
- Nausea and vomiting management
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Case Reports on Hematomas
- Alkaline Phosphatase Research Studies
- Liver Diseases and Immunity
- Enzyme Production and Characterization
Beijing National Laboratory for Molecular Sciences
2021-2025
Peking University
2021-2025
Hainan University
2025
Center for Life Sciences
2021-2024
Baogang Group (China)
2024
University of Washington
2018-2021
Seattle University
2019-2021
Howard Hughes Medical Institute
2020
Chinese Academy of Sciences
2006-2015
Institute of Biophysics
2011-2015
The major facilitator superfamily (MFS) is the largest family of secondary active transporters and present in all life kingdoms. Detailed structural basis substrate transport energy-coupling mechanisms these proteins remain to be elucidated. YajR a putative proton-driven MFS transporter found many Gram-negative bacteria. Here we report crystal structure Escherichia coli at 3.15 Å resolution an outward-facing conformation. In addition having 12 canonical transmembrane helices, includes unique...
The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry essential nutrients while excluding toxic compounds, including antibiotics. OM is asymmetric and contains an leaflet lipopolysaccharides (LPS) or lipooligosaccharides (LOS) inner glycerophospholipids (GPL). We screened Acinetobacter baumannii transposon mutants identified number with defects, ABC transporter system homologous to the Mla in E. coli. further show this opportunistic,...
The midgut microbiota of Aedes aegypti is crucial for the mosquito’s development, nutrition, and immunity. However, its communities are also distinctively influenced by colonization different microorganisms, influencing susceptibility to pathogens transmission capacity. In this study, we investigated effects infections with Escherichia coli, Staphylococcus aureus, Beauveria bassiana on microbial composition Ae. aegypti. These microorganisms were inoculated into midguts third-instar larvae...
In the era of global climate change, increasingly severe Fusarium head blight (FHB) and deoxynivalenol (DON) contamination have caused economic losses brought food feed safety concerns. Recently, an FHB resistance gene Fhb7 coding a glutathione-S transferase (GST) to degrade DON by opening critical toxic epoxide moiety was identified opened new window for wheat breeding detoxification. However, poor stability elusiveness catalytic mechanism hinder its practical application. Herein, we report...
Abstract The nonselective calcium‐permeable Transient Receptor Potential Cation Channel Subfamily V Member4 (TRPV4) channel regulates various physiological activities. Dysfunction of TRPV4 is linked to many severe diseases, including edema, pain, gastrointestinal disorders, lung and inherited neurodegeneration. Emerging antagonists show potential clinical benefits. However, the molecular mechanisms antagonism remain poorly understood. Here, cryo‐electron microscopy (cryo‐EM) structures human...
The urate transporter 1 (URAT1) is the primary in kidney responsible for reabsorption and, therefore, crucial homeostasis. Hyperuricemia causes common human disease gout and other pathological consequences. Inhibition of through URAT1 has been shown as a promising strategy alleviating hyperuricemia, clinical preclinical drug candidates targeting are emerging. However, how small molecules inhibit remains undefined, lack accurate complex structures hinders development better therapeutics....
Significance Phosphatases regulate many aspects of cellular function-homeostasis and signal transduction. Using X-ray crystallography methods, we determined the structure phosphatidylglycerol-phosphate phosphatase B (PgpB) from Escherichia coli , a member type II phosphatidic acid (PAP2) family homologue human glucose-6-phosphatase, which plays variety physiopathological roles. Our PgpB showed that membrane-integrated soluble members PAP2 share same catalytic mechanism. The mechanism...
Abstract Multi-resistant bacteria are a major threat in modern medicine. The gram-negative coccobacillus Acinetobacter baumannii currently leads the WHO list of pathogens critical need for new therapeutic development. maintenance lipid asymmetry (MLA) protein complex is one core machineries that transport lipids from/to outer membrane bacteria. It also contributes to broad-range antibiotic resistance several pathogens, most prominently A. . Nonetheless, molecular details its role has...
The sodium-activated Slo2.2 channel is abundantly expressed in the brain, playing a critical role regulating neuronal excitability. Na+-binding site and underlying mechanisms of Na+-dependent activation remain unclear. Here, we present cryoelectron microscopy (cryo-EM) structures human closed, open, inhibitor-bound form at resolutions 2.6-3.2 Å, revealing gating regulation by cations potent inhibitor. cytoplasmic ring domain closed harbors multiple K+ Zn2+ sites, which stabilize...
Abstract Biosynthetic enzymes evolutionarily gain novel functions, thereby expanding the structural diversity of natural products to benefit host organisms. Diels-Alderases (DAs), functionally unique catalysing [4 + 2] cycloaddition reactions, have received considerable research interest. However, their evolutionary mechanisms remain obscure. Here, we investigate origins intermolecular DAs in biosynthesis Moraceae plant-derived Diels-Alder-type secondary metabolites. Our findings suggest...
Intracellular Salmonella use a type III secretion system (TTSS) to translocate effector proteins across the phagosome membrane and thus promote vacuole tubulation, resulting in intracellular survival. This work demonstrates that SseJ binds eukaryotic lipid transporter oxysterol binding protein 1 (OSBP1). directs OSBP1 endosomal compartment manner dependent on TTSS located pathogenicity island 2 (SPI2). localization is mediated by both another OSBP1-binding SPI2 translocated effector,...
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Transient Receptor Potential Vanilloid 1 (TRPV1) plays a central role in pain sensation and is thus an attractive pharmacological drug target. SAF312 potent, selective, non-competitive antagonist of TRPV1 shows promising potential treating ocular surface pain. However, the precise mechanism by which inhibits remains poorly understood. Here, we present cryo-EM structure human complex with SAF312, elucidating structural foundation its antagonistic effects on TRPV1. binds to vanilloid binding...
The Hainan gibbon (Nomascus hainanus) is one of the most endangered primates globally, threatened by habitat destruction, genetic diversity loss, and ecological competition. In this study, given critical role gut microbiota in host immune regulation nutrient metabolism, we investigated composition age-related variations gibbons. Using 16S rRNA sequencing, systematically microbial We collected 41 fecal samples from Tropical Rainforest National Park, covering three age groups: juveniles (4–6...
Voltage-gated sodium (NaV) channels initiate action potentials. Fast inactivation of NaV channels, mediated by an Ile-Phe-Met motif, is crucial for preventing hyperexcitability and regulating firing frequency. Here we present cryo-electron microscopy structure NaVEh from the coccolithophore Emiliania huxleyi, which reveals unexpected molecular gating mechanism channel fast independent motif. An N-terminal helix plugs into open activation gate blocks it. The binding pose stabilized multiple...
Abstract Multi-resistant bacteria are a major threat in modern medicine. The gram-negative coccobacillus Acinetobacter baumannii currently leads the WHO list of pathogens critical need for new therapeutic development. maintenance lipid asymmetry (MLA) protein complex is one core machineries that transport lipids from/to outer membrane bacteria. It also contributes to broad-range antibiotic resistance several pathogens, most prominently A. . Nonetheless, molecular details its role has...