A5036107089- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- Reconstructive Facial Surgery Techniques
- Reconstructive Surgery and Microvascular Techniques
- Pancreatic function and diabetes
- Kruppel-like factors research
- Nasal Surgery and Airway Studies
- Cellular transport and secretion
- Cancer-related gene regulation
- Invertebrate Immune Response Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Circadian rhythm and melatonin
- Connexins and lens biology
- TGF-β signaling in diseases
- Insect and Arachnid Ecology and Behavior
- Physiological and biochemical adaptations
- Zebrafish Biomedical Research Applications
- Olfactory and Sensory Function Studies
- Microtubule and mitosis dynamics
- Photoreceptor and optogenetics research
- Cleft Lip and Palate Research
- Ubiquitin and proteasome pathways
- Facial Nerve Paralysis Treatment and Research
- Wound Healing and Treatments
Wayne State University
2015-2023
Michigan United
2017
Gooch & Housego (United Kingdom)
2016
Cincinnati Children's Hospital Medical Center
2006-2015
University of Cincinnati
2008-2011
Oregon Medical Research Center
1997-2005
Steno Diabetes Centers
2005
La Jolla Alcohol Research
2005
Kansas City Public Schools
2005
Mayo Clinic
1994-2004
KLF11 (TIEG2) is a pancreas-enriched transcription factor that has elicited significant attention because of its role as negative regulator exocrine cell growth in vitro and vivo . However, functional the endocrine pancreas remains to be established. Here, we report, for first time, our knowledge, characterization glucose-inducible insulin gene. A combination random oligonucleotide binding, EMSA, luciferase reporter, chromatin immunoprecipitation assays shows binds promoter regulates...
Sp1-like zinc finger transcription factors are involved in the regulation of cell growth and differentiation. Recent evidence demonstrating that mammalian cells express novel, yet uncharacterized, proteins has stimulated a search for new members this family. We others have recently reported transforming factor (TGF)-beta-regulated gene TIEG encodes protein inhibits cultured cells. Here we report identification, nuclear localization, DNA binding activity, transcriptional repression inhibitory...
Dynamin is a 100-kDa microtubule-activated GTPase originally isolated from mammalian brain that has been proposed to be crucial in the early steps of endocytosis. Previous studies on primary structure, biochemical properties, and functional role dynamin indicated it was neuron-specific. However, using an antibody against synthetic peptide representing enzymatic region rat (D100), we identified protein doublet liver, suggesting exists as different isoforms are distinct counterpart. We then...
The cystic fibrosis transmembrane conductance regulator forms a chloride channel that is regulated by phosphorylation and intracellular ATP levels.The structure of the channel-forming domains undetermined.To identify residues lining this we substituted cysteine, one at time, for 9 consecutive (91-99) in M1 membrane-spanning segment.The cysteine substitution mutants were expressed Xenopus oocytes.W e determined accessibility engineered to charged, sulfhydryl-specific methanethiosulfonate...
The Drosophila eye is a mosaic that results from the stochastic distribution of two ommatidial subtypes. Pale and yellow ommatidia can be distinguished by expression distinct rhodopsins other pigments in their inner photoreceptors (R7 R8), which are implicated color vision. pale subtype contains ultraviolet (UV)-absorbing Rh3 R7 blue-absorbing Rh5 R8. UV-absorbing Rh4 green-absorbing Rh6 exclusive one rhodopsin per photoreceptor widespread phenomenon, although exceptions exist. mechanisms...
Signaling pathways are reused for multiple purposes in plant and animal development. The Hippo pathway mammals Drosophila coordinates proliferation apoptosis via the coactivator oncoprotein YAP/Yorkie (Yki), which is homeostatically regulated through negative feedback. In eye, cross-repression between kinase LATS/Warts (Wts) growth regulator Melted generates mutually exclusive photoreceptor subtypes. Here, we show that this all-or-nothing neuronal differentiation results from positive...
Sp1-like transcription factors are characterized by three highly homologous C-terminal zinc finger motifs that bind GC-rich sequences. These proteins behave as either activators or repressors and have begun to be classified into different subfamilies based upon the presence of conserved outside domain. This classification predicts share certain functional properties. TIEG1 TIEG2 constitute a new subfamily transforming growth factor-β-inducible whose also However, regions DNA-binding domain...
• Ptosis of the brow is a significant and often unrecognized portion aging upper face. It contributes to both cosmetic functional aging. Correction mandatory prior blepharoplasty in that problems may be worsened without elevating brow. Direct coronal browlifts are most common approach problem. Midforehead have been reserved for men with receding hairlines. We have, past three years, performed midforehead on all our patients needing ptotic correction. Our review 72 treated this way, including...
Abstract Background Recent genome sequence analysis in the red flour beetle Tribolium castaneum indicated that this highly crepuscular animal encodes only two single opsin paralogs: a UV-opsin and long wavelength (LW)-opsin; however, these animals do not encode blue (B)-opsin as most other insects. Here, we studied spatial regulation of LW- gene paralogs comparison to five retina Drosophila melanogaster . Results In situ hybridization reveals retina, contrast with insect retinas, constitutes...
Glial cells play structural and functional roles central to the formation, activity integrity of neurons throughout nervous system. In retina vertebrates, high energetic demand photoreceptors is sustained in part by Müller glia, an intrinsic, atypical radial glia with features common many glial subtypes. Accessory support also exist invertebrates, but which this function insect largely undefined. Using cell-restricted transcriptome analysis, here we show that ommatidial cone (aka Semper...
Abstract: Dynamin proteins are members of a recently described family GTPases involved in receptor‐mediated processes. To date, three different dynamin‐encoding genes have been identified mammalian tissues. I is expressed only neurons, whereas dynamin II ubiquitously expressed. A third isoform, III, was originally isolated from rat testis cDNA library and shown to be testis‐specific. However, here we report the cloning characterization III brain lung, demonstrating more extended pattern...
To examine the use of autologous free dermal fat grafts (FDFGs) in reconstruction soft-tissue facial contour defects, an 8-year, retrospective, computerized medical chart review was conducted for 21 patients who underwent with FDFGs.Section Facial Plastic and Reconstructive Surgery, Department Otolaryngology, Oregon Health Sciences University, Portland, or affiliated hospitals.Twenty-one identified were included retrospective evaluation. Follow-up periods ranged from 11 to 94 months. Five...
A major question in development is how different specialized cell types arise from a common progenitor. In the adult Drosophila compound eye, color discrimination achieved by UV-, blue- and green-sensitive photoreceptors (PRs). These PR subsets neuronal precursors called R7 R8 cells. Recent studies have demonstrated that R7-based UV-sensitive PRs require repression of R8-based blue/green-sensitive characteristics to properly develop. This mediated transcription factor Prospero (Pros). Here,...
• Many facial plastic surgeons use operative steroids to reduce postoperative edema and morbidity. This of is based more on theory anecdotal experience than controlled studies. We studied 49 patients undergoing rhinoplasty in a randomized, double-blind fashion evaluate the effects perioperative steroid use. found significantly less eyelid paranasal those receiving steroids. In addition, trends toward ecchymosis, intranasal edema, discomfort were noted. (<i>Arch Otolaryngol Head Neck...
The function and integrity of photoreceptor cells are dependent upon the creation maintenance specialized apical structures: membrane discs/outer segments in vertebrates rhabdomeres insects. We performed a molecular morphological comparison Drosophila Pph13 orthodenticle (otd) mutants to investigate transcriptional network controlling late stages rhabdomeric cell development function. Although Otd have been implicated rhabdomere morphogenesis, we demonstrate that it is necessary remove both...