A5101635450- Parathyroid Disorders and Treatments
- Genetic and Kidney Cyst Diseases
- Genetic Syndromes and Imprinting
- Autophagy in Disease and Therapy
- Magnesium in Health and Disease
- Biomedical Research and Pathophysiology
- Fibroblast Growth Factor Research
- Neurological Disease Mechanisms and Treatments
- Ion Transport and Channel Regulation
- Dialysis and Renal Disease Management
- Pancreatic function and diabetes
- Acute Kidney Injury Research
- Medical Imaging and Pathology Studies
- Chronic Kidney Disease and Diabetes
- Advanced Glycation End Products research
- Neuroinflammation and Neurodegeneration Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Vitamin D Research Studies
- Electrolyte and hormonal disorders
- RNA Interference and Gene Delivery
- Cardiac Fibrosis and Remodeling
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Neurotransmitter Receptor Influence on Behavior
- Neurological Disorders and Treatments
Beijing Luhe Hospital Affiliated to Capital Medical University
2025
The University of Texas Southwestern Medical Center
2015-2024
Dongyang People's Hospital
2024
Kangda College of Nanjing Medical University
2024
Wenzhou Medical University
2024
Nanjing Medical University
2024
Zhejiang Environmental Monitoring Center
2023
Hebei General Hospital
2016-2022
Chinese PLA General Hospital
2021
Binghamton University
2021
Soft-tissue calcification is a prominent feature in both chronic kidney disease (CKD) and experimental Klotho deficiency, but whether deficiency responsible for the CKD unknown. Here, wild-type mice with had very low renal, plasma, urinary levels of Klotho. In humans, we observed graded reduction starting at an early stage progressing loss renal function. Despite induction CKD, transgenic that overexpressed preserved Klotho, enhanced phosphaturia, better function, much less compared CKD....
Klotho has profound effects on phosphate metabolism, but the mechanisms of how affects homeostasis is unknown. We detected in proximal tubule cell, brush border, and urinary lumen, where resides. Increasing kidney urine chronically by transgenic overexpression or acutely intravenous infusion caused hypophosphatemia, phosphaturia from decreased reabsorption, activity protein principal renal transporter NaPi-2a. The phosphaturic effect was present FGF23-null mice, indicating a direct action...
Fibroblast growth factor (FGF) 23 inhibits renal phosphate reabsorption by activating FGF receptor (FGFR) 1c in a Klotho-dependent fashion. The phosphaturic activity of FGF23 is abrogated proteolytic cleavage at the RXXR motif that lies boundary between core homology domain and 72-residue-long C-terminal tail FGF23. Here, we show soluble ectodomains FGFR1c Klotho are sufficient to form ternary complex with vitro. mediates binding de novo site generated composite FGFR1c-Klotho interface....
Cardiac dysfunction in CKD is characterized by aberrant cardiac remodeling with hypertrophy and fibrosis. a state of severe systemic Klotho deficiency, restoration attenuates vascular calcification associated CKD. We examined the role models deficiency—genetic hypomorphism, high dietary phosphate intake, aging, Klotho-deficient mice exhibited before 12 weeks age followed In wild-type mice, induction led to cardiovascular changes not observed control mice. Notably, non-CKD fed high-phosphate...
αKlotho is a multifunctional protein highly expressed in the kidney. Soluble released through cleavage of extracellular domain from membrane by secretases to function as an endocrine/paracrine substance. The role kidney circulating production and handling incompletely understood, however. Here, we found higher concentration suprarenal compared with infrarenal inferior vena cava both rats humans. In rats, serum dropped precipitously after bilateral nephrectomy or upon treatment inhibitors...
Low levels of the adipocyte-secreted protein adiponectin correlate with albuminuria in both mice and humans, but whether has a causative role modulating renal disease is unknown. Here, we first generated mouse model that allows induction caspase-8–mediated apoptosis specifically podocytes upon injection construct-specific agent. These POD-ATTAC exhibited significant kidney damage, mimicking aspects human disease, such as foot process effacement, mesangial expansion, glomerulosclerosis. After...
AKI confers increased risk of progression to CKD. αKlotho is a cytoprotective protein, the expression which reduced in AKI, but relationship level CKD has not been studied. We altered systemic levels by genetic manipulation, phosphate loading, or aging and examined effect on long-term outcome after two models: bilateral ischemia-reperfusion injury unilateral nephrectomy plus contralateral injury. Despite apparent initial complete recovery renal function, both types eventually progressed CKD,...
The 40-fold increase in childhood megakaryocyte-erythroid and B-cell leukemia Down syndrome implicates trisomy 21 (T21) perturbing fetal hematopoiesis. Here, we show that compared with primary disomic controls, T21 liver (FL) hematopoietic stem cells (HSC) progenitors are markedly increased, whereas granulocyte-macrophage reduced. Commensurately, HSC higher clonogenicity, increased megakaryocyte, megakaryocyte-erythroid, replatable blast colonies. Biased megakaryocyte-erythroid–primed gene...
αKlotho is the prototypic member of Klotho family and most highly expressed in kidney. has pleiotropic biologic effects, kidney, its actions include regulation ion transport, cytoprotection, anti-oxidation anti-fibrosis. In rodent models chronic kidney disease (CKD), deficiency been shown to be an early biomarker as well a pathogenic factor. The database for human CKD remains controversial even after years study. We used synthetic antibody library identify high-affinity antigen-binding...
α-Klotho exerts pleiotropic biological actions. Heterozygous haplo-insufficient mice (kl/+) appear normal at baseline except for age-related changes in the lung, suggesting heightened pulmonary susceptibility to deficiency. We used vivo and vitro models test whether protects lung epithelia against injury. Normally, is not expressed but circulating levels are reduced -40% kl/+ undetectable homozygous α-Klotho-deficient (kl/kl). show distal air space enlargement a given airway pressure, with...
Parkinson's disease (PD) is a progressive neurodegenerative which characterized by the degeneration of dopaminergic neurons in substantia nigra pars compacta (SNpc).