A5038373261- Cardiac Imaging and Diagnostics
- Cardiac Ischemia and Reperfusion
- Acute Myocardial Infarction Research
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Melanoma and MAPK Pathways
- Coronary Interventions and Diagnostics
- Cardiac Fibrosis and Remodeling
- Cardiac Arrest and Resuscitation
- Advanced MRI Techniques and Applications
- Protein Kinase Regulation and GTPase Signaling
- Cardiovascular Effects of Exercise
- Heat shock proteins research
- Cardiovascular Health and Disease Prevention
- Cardiovascular Disease and Adiposity
- Signaling Pathways in Disease
- Anesthesia and Neurotoxicity Research
- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Mitochondrial Function and Pathology
- Cardiovascular and exercise physiology
- Viral Infections and Immunology Research
- Nitric Oxide and Endothelin Effects
- Cardiomyopathy and Myosin Studies
- Adipose Tissue and Metabolism
King's College London
2016-2025
St Thomas' Hospital
2015-2024
British Heart Foundation
2015-2024
King's College Hospital
1999-2024
Guy's and St Thomas' NHS Foundation Trust
2003-2024
National Institute for Health Research
2012-2024
King's College School
2008-2024
University of Kansas Medical Center
2024
University of Oslo
2023
NIHR Biomedical Research Centre at The Royal Marsden and the ICR
2012-2021
BACKGROUND To test the hypothesis that heat shock response is associated with myocardial salvage, stress protein (HSP) content of cardiac tissue was increased by either ischemic or thermal stress. METHODS AND RESULTS Rabbits were divided into four groups. Ischemic pretreatment (n = 15) comprised 5-minute episodes coronary ligation separated 10 minutes reperfusion. The corresponding control group 21) underwent surgical preparation without ligation. Thermal 16) involved whole-body temperature...
Myocardial protection and changes in gene expression follow whole body heat stress. Circumstantial evidence suggests that an inducible 70-kD shock protein (hsp70i), increased markedly by stress, contributes to the protection. Transgenic mouse lines were constructed with a cytomegalovirus enhancer beta-actin promoter driving rat hsp70i heterozygote animals. Unstressed, transgene positive mice expressed higher levels of myocardial than negative after This high level occurred without apparent...
In patients with stable angina, two strategies are often used to guide revascularization: one involves myocardial-perfusion cardiovascular magnetic resonance imaging (MRI), and the other invasive angiography measurement of fractional flow reserve (FFR). Whether a MRI-based strategy is noninferior an FFR-based respect major adverse cardiac events has not been established.We performed unblinded, multicenter, clinical-effectiveness trial by randomly assigning 918 typical angina either or more...
Coronary microvascular dysfunction (MVD) is defined by impaired flow augmentation in response to a pharmacological vasodilator the presence of nonobstructive coronary artery disease. It unknown whether diminished correlates with abnormal exercise physiology or inducible myocardial ischemia.
Exercise electrocardiographic stress testing (EST) has historically been validated against the demonstration of obstructive coronary artery disease. However, myocardial ischemia can occur because microvascular dysfunction (CMD) in absence The aim this study was to assess specificity EST detect an ischemic substrate reference standard endothelium-independent and endothelium-dependent function patients with angina nonobstructive arteries (ANOCA). Patients ANOCA underwent invasive physiological...
BACKGROUND We previously reported a delayed phase of protection against infarction 24 hours after ischemic preconditioning in the rabbit. In present study, we investigated possibility that this "second window protection," like well-described early rabbit, might be associated with adenosine receptor activation. METHODS AND RESULTS first series experiments, examined whether blockade 8-(p-sulfophenyl)-theophylline (SPT) during could abolish later. Open-chest rabbits were subjected to myocardial...
Oxidative stress plays an important role in the development of cardiac remodeling after myocardial infarction (MI), but sources oxidative remain unclear. We investigated Nox2-containing reduced nicotinamide-adenine dinucleotide phosphate oxidase MI. Adult Nox2(-/-) and matched wild-type (WT) mice were subjected to coronary artery ligation studied 4 weeks later. Infarct size MI was similar WT mice. exhibited significantly less left ventricular (LV) cavity dilatation dysfunction than (eg,...
We show here that exposure of cardiac cells to simulated ischemia results in apoptosis and is accompanied by phosphorylation increased expression transcriptional activity STAT-1. Similarly, interferon-γ, which known induce STAT-1 activation, also induced cells. STAT-1-transfected were more susceptible ischemia-induced cell death than transfected with a control plasmid lacking the coding sequence. Furthermore, an antisense vector reduced both ischemia- overexpressed STAT-1-induced Both...
Complement research experienced a renaissance with the discovery of third activation route, lectin pathway. We developed unique model total pathway deficiency, mouse strain lacking mannan-binding lectin-associated serine protease-2 (MASP-2), and analyzed role MASP-2 in two models postischemic reperfusion injury (IRI). In transient myocardial IRI, MASP-2–deficient mice had significantly smaller infarct volumes than their wild-type littermates. Mice deficient downstream complement component C4...
Exercise markedly influences pulse wave morphology, but the mechanism is unknown. We investigated whether effects of exercise on arterial result from alterations in stroke volume or velocity (PWV)/large artery stiffness reduction pressure reflection. Healthy subjects (n = 25) performed bicycle ergometry. with workload increasing 25 to 150 W for 12 min. Digital waveforms were recorded using a servo-controlled finger cuff. Radial and carotid-femoral PWV determined by applanation tonometry....
Peripheral systolic blood pressure is amplified above central aortic pressure, but the late shoulder of peripheral pulse may approximate pressure. Because also determines augmentation index, a measure wave reflection within systemic circulation, this implies direct relationship between amplification and augmentation. We compared waveform with estimates obtained using transfer function in 391 subjects undergoing diagnostic coronary angiography and/or elective angioplasty (30% insignificant...
The objective of this study was to examine determinants excess coronary artery disease risk in UK South Asians, more prevalent population than Caucasians, by examining differences factors, vascular function, and endothelial progenitor cells (EPCs).24 Asian 25 Caucasian healthy age-matched nonsmoking men were studied. Vascular function assessed flow-mediated GTN brachial dilatation blood flow responses infusion ACh, SNP, L-NMMA. EPC number measured cytometry (CD34, CD133, KDR positive cells),...
Noncoding RNAs (ncRNAs), including microRNAs (miRNAs), circular (circRNAs), and long noncoding (lncRNAs), are proposed novel biomarkers of myocardial injury. Their release kinetics have not been explored without confounding by heparin nor has their relationship to protein biomarkers.
Abstract A substantial number of chronic coronary syndrome (CCS) patients undergoing percutaneous intervention (PCI) experience periprocedural myocardial injury or infarction. Accurate diagnosis these PCI-related complications is required to guide further management given that their occurrence may be associated with increased risk major adverse cardiac events (MACE). Due lack scientific data, the cut-off thresholds post-PCI troponin (cTn) elevation used for defining and infarction, have been...
Coronary microvascular resistance is increasingly measured as a predictor of clinical outcomes, but there no accepted gold-standard measurement. We compared the diagnostic accuracy 2 invasive indices resistance, Doppler-derived hyperemic (hMR) and thermodilution-derived index microcirculatory (IMR), at predicting dysfunction. A total 54 patients (61 ± 10 years) who underwent cardiac catheterization for stable coronary artery disease (n = 10) or acute myocardial infarction 44) had...