ABSTRACT The Charles procedure (CP) is a potentially devastating treatment; however, in cases of an end stage untreated or improperly treated lymphedema, it the ultimate surgical therapy. As life‐saving solution, quickly relieves patients with giant, hypertrophic extremities, mostly ambulation and hygiene maintenance. Nevertheless, long‐term results may disappoint both doctors patients, who struggle social stigma, need for lifelong compression, massive lymphoedema distal parts feet, badly...

Introduction: Upper limb lymphedema is the most common complication after breast cancer therapy. Suddenly disturbed lymphatic transport in affected arm causes tissue fluid accumulation spaces, enlargement, and secondary changes tissue. Early compression therapy necessary. We aim to evaluate effectiveness of intermittent pneumatic (IPC) movement along possibility creating compensatory drainage pathways. Methods Results: investigated 25 patients with Breast Cancer Related Lymphedema (BCRL)...

Currently, the number of stem-cell based experimental therapies in neurological injuries and neurodegenerative disorders has been massively increasing. Despite fact that we still have not obtained strong evidence mesenchymal stem/stromal cells' neurogenic effectiveness vivo, research may need to focus on more appropriate sources result therapeutically promising cell populations. In this study, used dedifferentiated fat cells (DFAT) are proven demonstrate pluripotent abilities comparison with...

Background: Carpal tunnel release is the gold standard for treatment of median nerve compression disease. Recurrent or persistent symptoms do not occur in most patients, although a small number them have indicated that such postoperative condition indeed exists. Some patients undergo repeated treatments. In majority cases, disease associated with scarring carpal even reformation ligament. The authors propose usage autologous fat grafting during secondary to inhibit process. Methods: Ten...

Objective/Purpose. Evaluation of efficacy and safety autologous adipose-derived regenerative cells (ADRCs) treatment in autoimmune refractory epilepsy. Patients. Six patients with proven or probable epilepsy (2 Rasmussen encephalitis, 2 antineuronal autoantibodies serum, possible FIRES) were included the project approval Bioethics Committee.Intrathecal injection ADRC acquired through liposuction followed by enzymatic isolation was performed. The procedure repeated 3 times every months each...

The development of an effective method melanocyte isolation and culture is necessary for basic clinical studies concerning skin diseases, including pigmentation disorders melanoma. In this paper, we describe a novel, non-enzymatic metastatic melanoma cell (along with the spontaneous spheroid creation) from or lymph node explants. based on selective harvesting melanocytes cells emigrating cultured Thereby, isolated retain their natural phenotypical features, such as expression tyrosinase...

Stem cells (SCs) may constitute a perspective alternative to pharmacological treatment in neurodegenerative diseases. Although the safety of SC transplantation has been widely shown, their clinical efficiency amyotrophic lateral sclerosis (ALS) is still be proved. It not only due limited number studies, small groups, and fast but nonlinear disease progression also lack objective methods able show subtle changes. Preliminary guidelines for cell therapy have recently proposed by group ALS...

ABCA4 gene mutations are the cause of a spectrum retinopathies, and most common juvenile macular degeneration is called Stargardt disease. has previously been observed almost exclusively in retina. Therefore, studying functional consequences variants required advanced molecular analysis techniques. The aim present study was to evaluate whether human hair follicles may be used for splice-site patients with retinopathies. We assessed expression skin at mRNA protein levels by means real-time...

The reconstruction of nerve continuity after traumatic injury is the gold standard in hand surgery. Immediate, tension-free, end-to-end suture ensures best prognosis. recovery mostly promising; however, a few cases, insufficient outcomes motor or sensory function are observed. Intra- and extra-fascicular scarring accompanies regeneration process limits final outcomes. Secondary release those cases recommended. Unfortunately, recurrence cannot be eliminated secondary revision neurolysis....

Introduction: In advanced lymphedema of lower limbs, stage III bandaging under the routinely applied pressure 40–60 mmHg remains largely ineffective. This is caused by skin and subcutaneous tissue stiffness due to fibrosis. Edema fluid accumulates deep in tissue. Evacuating this requires a high external compression force overcome resistance fibrous We aimed investigate effectiveness method, with lasting for 3 days.

A universal classification of the negative outcomes carpal tunnel release surgery does not, as yet, exist. In order to avoid use arbitrary factors we have applied Accordion Severity Grading System, which uses rigorously defined qualitative terms classify complications. It also provides a uniform manner for grading severity complications, enabling outcome comparisons between centres. We analysed 500 device-assisted releases performed over 2-year period in author's department. establish...

Background: Lipedema of lower limbs is characterized by bilateral accumulations excess adipose tissue starting from the ankle to hips and buttocks. The studies with lymphoscintigraphy (LSC) magnetic resonance (MR) lymphography show altered transport index enlarged lymphatic vessels (LVs). Our aimed investigate superficial lymph flow, water accumulation, skin subcutaneous elasticity, possibility using this information diagnose lipedema. Methods Results: Fifty patients lipedema 50 control...

The epidermis forms the barrier between an organism and its external environment. Although one of major functional elements is lipid-enriched extracellular matrix, containing mainly ceramides, cholesterol (CHOL) free fatty acids, data are limited regarding lipid profile in epidermis. aim study was to determine whole acids (FAs) examine any dependence according age subject site on epidermis.Epidermis extracts obtained from 10 adults 6 children were analyzed by gas chromatography-mass...

Ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5mC), first to 5-hydroxymethylcytosine (5hmC), then 5-formylcytosine (5fC), and finally 5-carboxycytosine (5caC). Evidence suggests that changes in TET expression may impact cell function phenotype aging. Proliferation, apoptosis, markers autophagy double-strand DNA break repair, Fibulin 5 were assessed by flow cytometry TET1 TET2-overexpressing fibroblasts isolated from sun-unexposed skin young (23-35 years)...

Introduction: Lymph flows along the lymphatics due to spontaneous contraction. However, injury and inflammation may deteriorate lymphatic' s endothelial muscle cells valves. In consequence, lymphatic vessels (LVs) become insufficient. Their contraction strength rate slow down, then lymph flow stops. Our study aimed investigate changes in early lymphedema cases. Methods Results: 36 patients with unilateral stages 0 I, we performed indocyanine green (ICG) lymphography, lymphoscintigraphy, skin...

Colon anastomosis is therapeutically challenging because multiple, usually undetectable factors influence a spectrum of repair mechanisms. We hypothesized that low molecular weight heparins, routinely administered perioperatively, may differentially affect gene expression related to colon healing. Twenty pairs untreated and enoxaparin-treated rats underwent left-side hemicolectomy with primary end-to-end anastomosis. Normal anastomotic bowel segments were resected on day 0 days 1, 3, 5, 7...

Abstract The application of mesenchymal stem/stromal cells (MSC) in regenerative medicine offers hope for the effective treatment incurable or difficult‐to‐heal diseases. However, it requires development unified protocols both safe and efficient cell acquisition clinical usage. therapeutic effect fat grafts (containing stem cells) non‐healing wounds has been discussed previous studies, although local general anaesthesia. MSC derived from adipose tissue (ASC) could be a less invasive method,...

Transplantation of autogenic adipose tissue by means the suction technique “anesthesia with edema”, has been for past years one most engaged currents contemporary plastic surgery. It is directly associated two issues described below. The first discovery peripheral fat progenitor cells characterized their in vitro plasticity (1). These are now referred to as Adipose Derived Stromal Cells (ADSC), being subjected culture differentiation different tissues, such fat, cartilage, bone, or neural...

In this publication we describe three techniques for endoscopic carpal tunnel release which use the palmar portal approach and superficial plane to enable a view from above transverse ligament. These prevent any iatrogenic compression of median nerve inside already narrow tunnel. Although necessary instruments are inexpensive recommended in instrumentation catalogues, their has rarely been reported. safe easy learn surgeons familiar with arthroscopy. We believe that they constitute an...

BACKGROUND: The modified version of the Clavien-Dindo classification complications has been renamed Accordion Severity Grading System.1 Since its introduction it received international acceptance in various surgical specialties. However, never used aesthetic surgery. System enables a simple assessment and comparison outcomes between procedures centres. We propose using surgery to provide common language for research discussion where real can be distinguished from failures, or as...

The ABCA4 gene encodes an ATP-binding cassette transporter that is expressed specifically in the disc of photoreceptor outer segments. Mutations are main cause retinal degenerations known as “ABCA4-retinopathies.” Recent research has revealed other cells well, such hair follicles and keratinocytes, although no information on its significance been evidenced so far. In this study, we investigated role human keratinocytes follicle stem for first time. We have shown silencing increases...

Lower limb trauma often results in mangled extremities, and some cases, complete amputation may be necessary. However, limiting the extent of preserving major knee joint are crucial to enhance mobility overall functionality. By providing painless soft tissue coverage on stump, early prosthesis use initiation physiotherapy become more feasible. Soft transfers hold potential benefit patients two essential aspects: first, resolving deficiencies without causing bone shortening, second, preparing...