A5077930196- Optical Imaging and Spectroscopy Techniques
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Thermoregulation and physiological responses
- Infrared Thermography in Medicine
- Cellular transport and secretion
- Pancreatic function and diabetes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nanoplatforms for cancer theranostics
- Traumatic Brain Injury and Neurovascular Disturbances
- Ocular and Laser Science Research
- Laser Applications in Dentistry and Medicine
- Single-cell and spatial transcriptomics
- Hepatitis C virus research
- Genetic Mapping and Diversity in Plants and Animals
- Hepatitis B Virus Studies
- Genetic and phenotypic traits in livestock
- Photoreceptor and optogenetics research
- Dermatologic Treatments and Research
- Animal Nutrition and Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular Mechanics and Interactions
- Photodynamic Therapy Research Studies
Wuhan National Laboratory for Optoelectronics
2016-2025
Huazhong University of Science and Technology
2016-2025
Minzu University of China
2025
China University of Petroleum, East China
2025
Union Hospital
2018-2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
Jingchu University of Technology
2024
BOE Technology Group (China)
2018-2023
Saratov State University
2023
Abstract Tissue optical clearing technique provides a prospective solution for the application of advanced methods in life sciences. This paper gives review recent developments tissue techniques. The physical, molecular and physiological mechanisms are overviewed discussed. Various enhancing penetration optical‐clearing agents into tissue, such as physical methods, chemical‐penetration enhancers combination chemical introduced. Combining with microscopy image or labeling technique,...
We developed an advanced clearing method with superior fluorescence-preserving capability for 3D imaging of whole organs.
Significance Genomic prediction is a new field of quantitative genetics. Individual performance can be predicted using genome-wide markers before the phenotype measured. for hybrid even more promising because genotype predetermined by parents. We propose genomic best linear unbiased to predict rice, and incorporate dominance epistasis into model. Simulation studies showed that predictability further improved after incorporating The strategy marker-guided called breeding, it represents...
The penetration of light in biological tissues can be enhanced by using optical-clearing techniques. However, researches on the mechanism are limited to vitro experiments. In this study, rat dorsal skin was used discuss for vivo optical clearing. Glycerol solutions with different concentrations were applied dermal injection; then reflectance spectrum, SHG imaging and microstructural changes monitored. results showed that becoming transparent, corresponding decreased, thickness dermis...
The production of soybean products has been increasing throughout the world, and there a corresponding increase in quantity curd residue (SCR) being thrown out. dumping SCR become problem to be solved due its contamination environment. is rich fiber, fat, protein, vitamins, trace elements. It potential for value-added processing utilization; options that simultaneously hold promise increased economic benefit as well decreased pollution objective this study fully investigate, review,...
Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us repeatedly image neurons, microglia mice. We applied it study plasticity dendritic spines critical periods visualize dendrites after laser ablation. Given its easy handling safety, method holds great...
The abnormal angiogenesis and insufficient oxygen supply in solid tumors lead to intratumoral hypoxia, which severely limits the efficacy of traditional photodynamic therapy (PDT). Here, a multifunctional nanoplatform (ZDZP@PP) based on zeolitic imidazolate framework-67 (ZIF-67) core as hydrogen peroxide catalyst, framework-8 (ZIF-8) shell with pH-responsive property, polydopamine–poly(ethylene glycol) (PDA–PEG) layer for improving biocompatibility is fabricated not only relieving tumor...
Intravital optical imaging is a significant method for investigating cerebrovascular structure and function. However, its contrast depth are limited by the turbid skull. Tissue clearing has great potential solving this problem. Our goal was to develop transparent skull window, without performing craniotomy, use in assessing
Abstract Tissue optical clearing techniques have provided important tools for large‐volume imaging. Aqueous‐based methods are known good fluorescence preservation and scalable size maintenance, but limited by long incubation time, insufficient performance, or requirements specialized devices. Additionally, few compatible with widely used lipophilic dyes while maintaining high performance. Here, to address these issues, m‐xylylenediamine (MXDA) is firstly introduced into tissue develop a...
Responsive nanocarriers with biocompatibility and precise drug releasing capability have emerged as a prospective candidate for anticancer treatment. However, the challenges imposed by complicated preparation process limited loading capacities seriously impeded development of novel multifunctional delivery systems. Here, we developed dual-responsive nanocarrier based on nanoscale ZIF-8 core an organosilica shell containing disulfide bridges in its frameworks through facile efficient...
Investigating the genetic markers and genomic signatures related to chicken meat production by combing multi-omics methods could provide new insights into modern breeding technology systems. Chicken is one of most efficient environmentally friendly livestock, especially fast-growing white-feathered (broiler), which well known for high yield, but underlying basis poorly understood. We generated whole-genome resequencing three purebred broilers (n = 748) six local breeds/lines 114), sequencing...
Abstract Intraventricular hemorrhage is one of the most fatal forms brain injury that a common complication premature infants. However, therapy this type limited, and new strategies are needed to reduce hematoma expansion. Here we show meningeal lymphatics pathway remove red blood cells from brain’s ventricular system male human, adult newborn rodents target for non-invasive transcranial near infrared photobiomodulation. Our results uncover clinical significance phototherapy intraventricular...
Alzheimer's disease (AD) and related Tauopathies are characterised by the pathologically hyperphosphorylated aggregated microtubule-associated protein Tau, which is accompanied neuroinflammation mediated activated microglia. However, role of Tau pathology in microglia activation or their causal relationship remains largely elusive.
Abstract Optical imaging plays a central role in the field of biomedicine, but it suffers from light scattering tissues. The research group Stanford University has reported counterintuitive observation that strongly absorbing molecules could achieve optical transparency live animals, providing new insight for understanding tissue clearing. It empowers scientists to leverage techniques vivo wide range deep-seated structures and activities.
Noninvasive detection of skin microcirculation is very significant for clinical diagnosis and therapy peripheral vascular disease. In this study, an optical clearing method enables a laser speckle contrast imaging (LSCI) technique to image dermal blood flow through intact rat skin. The effect <i>in vitro</i> caused by mixture PEG-400 Thiazone evaluated commercially available spectrophotometer with integrating sphere. A LSCI system applied the vivo</i> after topical treatment agents. results...
The strong optical scattering of skin tissue makes it very difficult for coherence tomography (OCT) to achieve deep imaging in skin. Significant clearing vivo rat sites was achieved within 15 min by topical application an agent PEG-400, a chemical enhancer (thiazone or propanediol), and physical massage. Only when all three components were applied together could treatment fold increase the OCT reflectance from 300 μm depth 31% enhancement image Z(threshold).
Dehydration induced by optical clearing agents (OCAs) can improve tissue transmittance; however, current studies merely gave some qualitative descriptions. We develop a model to quantitatively evaluate water content with partial least-squares method based on the measurements of near-infrared reflectance spectroscopy and weight porcine skin. Furthermore, commercial spectrometer an integrating sphere is used measure transmittance skin after treatment different OCAs, then properties sample are...
Noninvasive optical methods for viewing the structural and functional organization of cortex have been playing important roles in brain research, which usually suffer from turbid skull. Various cranial window models based on surgical operation proposed, but respective limitations. Here, an innovative transparent mouse was established by topically treatment with a skull clearing solution (SOCS), rather than craniotomy. Based experiment efficacy vitro, we found that became within 25 min after...
Recently, a variety of tissue optical clearing techniques have been developed to reduce light scattering for imaging deeper and three-dimensional reconstruction structures. Combined with diverse labeling methods, these methods significantly promoted the development neuroscience. Each them has its own characteristics certain advantages disadvantages. Though there are some comparison results, covered limited evaluation indices lack uniformity, which made it difficult select best-fit protocol...