A5030122703- Silk-based biomaterials and applications
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Knee injuries and reconstruction techniques
- Tendon Structure and Treatment
- Osteoarthritis Treatment and Mechanisms
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Mesenchymal stem cell research
- Total Knee Arthroplasty Outcomes
- Orthopedic Surgery and Rehabilitation
- Muscle Physiology and Disorders
- Shoulder Injury and Treatment
- Microplastics and Plastic Pollution
- Laser Applications in Dentistry and Medicine
- Cellular Mechanics and Interactions
- Polymer Surface Interaction Studies
- Periodontal Regeneration and Treatments
- Angiogenesis and VEGF in Cancer
- Hernia repair and management
- Silkworms and Sericulture Research
- Burn Injury Management and Outcomes
- Shoulder and Clavicle Injuries
- RNA Interference and Gene Delivery
Austrian Cluster for Tissue Regeneration
2016-2025
University of Applied Sciences Technikum Wien
2016-2025
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2009-2020
Creative Commons
2018
Tufts University
2016
Shock wave treatment accelerates impaired wound healing in diverse clinical situations. However, the mechanisms underlying beneficial effects of shock waves have not yet been fully revealed. Because cell proliferation is a major requirement cascade, we used vitro studies and an vivo model to study whether influences by altering extracellular factors signaling pathways involved proliferation. We identified ATP, released energy- pulse number-dependent manner, as trigger biological treatment....
Decellularization of native blood vessels is a promising technology to generate 3D biological scaffolds for vascular grafting. Blood vessel decellularization has been performed in previous studies under various experimental conditions, that complicates comparison and optimization suitable protocols. The goal this work was systematically compare the recellularization efficacy 5 different protocols utilizing detergents sodium dodecyl sulfate (SDS), deoxycholate (SDC), CHAPS TritonX-100...
Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due the poor self-healing capacity affected often exhibits osteoarthritic changes. In recent years, injectable highly tunable composite hydrogels for engineering drug delivery have been introduced as a desirable alternative invasive treatments. this study, we aimed formulate by combining different concentrations hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions. Upon enzymatic...
Biomechanical cues such as shear stress, stretching, compression, and matrix elasticity are vital in the establishment of next generation physiological vitro tissue models. Matrix elasticity, for instance, is known to guide stem cell differentiation, influence healing processes modulate extracellular (ECM) deposition needed development maintenance. To better understand biomechanical effect on formation articular cartilage analogs vitro, this study aims at assessing redifferentiation capacity...
The human meniscus transmits high axial loads through the knee joint. This function is compromised upon injury or treatment by meniscectomy. 3D printing of implants has emerged as a promising alternative treatment, it allows for precise mimicry architecture. In this study, silk fibroin (SF) known its excellent mechanical properties used to fabricate hydrogels bioprinting with infrapatellar fat pad-derived mesenchymal stem cells (IFP-MSCs). Extracellular matrix (ECM) derived from bovine...
We introduce a unique bioanalytic hybrid system for the preclinical assessment of radiotracer candidates, combining 3-dimensional cell culture on an artificial extracellular matrix functioning as stationary phase and chromatographiclike array. Methods: Silk fibroin sponges were applied to simulate function phase. Different lines grown silk scaffold used investigate radiopharmaceuticals in small-animal PET/CT system. Results: Our integrates with strength chromatographic systems, allowing high...
There is a great clinical need and it remains challenge to develop artificial soft tissue constructs that can mimic the biomechanical properties bioactivity of natural tissue. This partly due lack suitable biomaterials. Hydrogels made from human placenta offer high represent potential solution create animal-free 3D bioprinting systems are both sustainable acceptable, as widely considered medical waste. A combination with silk gelatin polymers bridge limitations chorion extracellular matrix...
Background: Because of ongoing problems with anterior cruciate ligament (ACL) reconstruction, new approaches in the treatment ACL injuries, particularly strategies based on tissue engineering, have gained increasing research interest. To allow for regeneration, a structured scaffold that provides mechanical basis, has cells from different sources, and comprises as well biological factors is needed. Biological materials, biodegradable polymers, composite materials are being used tested...
Microcomputed tomography ( μ CT) is widely used for the study of mineralized tissues, but a similar use soft tissues hindered by their low X-ray attenuation. This limitation can be overcome recent development different staining techniques. Staining with Lugol’s solution, mixture one part iodine and two parts potassium iodide in water, stands out among these techniques its complexity cost. Currently, Lugol mostly anatomical examination tissues. In present study, we seek to optimize quality...
BACKGROUND An effective way of modulating wound healing processes, including proliferation and apoptosis, is low-level light therapy. Because several disadvantages lasers, light-emitting diodes (LEDs) could be more feasible sources. OBJECTIVE To evaluate compare the effects blue red from LEDs on different cell types in an vitro scratch-wound model. METHODS Monolayers C2C12 myoblasts, NIH/3T3 fibroblasts, BICR10 keratinocytes were injured by mechanical scraping. Cells illuminated 5...
Decellularization of blood vessels is a promising approach to generate native biomaterials for replacement diseased vessels. The decellularization process affects the mechanical properties vascular graft and thus can have negative impact in vivo functionality. aim this study was determine how detergents under different fluid dynamics efficacy graft. We applied protocol utilizing 1% TritonX, Tributyl phosphate (TnBP) DNase on porcine vena cava. were conditions; static, agitation perfusion...
Silk fibroin has previously been described as a promising candidate for ligament tissue engineering (TE) approaches. For biocompatibility reasons, silkworm silk requires removal of sericin, which can elicit adverse immune responses in the human body. One disadvantage required degumming process is alteration fiber structural properties, hinder textile high order hierarchical structures. Therefore, aim this study was to find way remove sericin from compact and highly ordered raw matrix. The...
Natural extracellular matrix-derived biomaterials from decellularized allogenic tissues are of increasing interest for tissue engineering because their structure and composition provide a complexity that is not achievable with current manufacturing techniques. The prerequisite to bring bench bedside as functional biomaterial the full removal cells while preserving most its native characteristics such composition. exceptionally dense articular cartilage, however, poses special challenge...
New antimicrobial pH-responsive wound dressings consisting of immobilized human serum albumin/silk fibroin nanocapsules were developed.
Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. loss associated repair failure after reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone density. Hypothesis: Zoledronic acid improves of and biomechanical properties enthesis reconstruction tears rats. Study Design: Controlled laboratory study. Methods: A total 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy delayed transosseous 3 weeks....
The additive manufacturing of low elastic modulus alloys that have a certain level porosity for biomedical needs is growing area research. Here, we show the results porous and dense samples by laser powder bed fusion (LPBF) Ti-Nb alloy, using two distinctive strategies. nanostructured alloy powders were produced mechanical alloying state with nanosized grains up to 90 nm. manufactured pronounced open advanced roughness, contrary relatively smooth surface profile. structure both types after...
Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated effects of each pathway on properties bone their capacity to drive regeneration. Bone-marrow-derived MSCs were differentiated silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks in vitro cultivation, implanted subcutaneously for 12 weeks. The pathways’ constructs time with respect gene...
The human meniscus experiences mechanical forces and converts axial loads into hoop stresses. Meniscus injuries meniscectomies can compromise this function, therefore, implants are required. To assess their performance in vitro, it is crucial to recreate a physiological environment. Therefore, we investigated the effect of TGFβ-3-supplemented TGFβ-free cyclic hydrostatic pressure (HP) up 10 MPa on 3D bioprinted silk fibroin (SF) polymer-based hydrogels. bioink was seeded with infrapatellar...