A5081301767- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Genomics, phytochemicals, and oxidative stress
- IL-33, ST2, and ILC Pathways
- Helicobacter pylori-related gastroenterology studies
- SARS-CoV-2 and COVID-19 Research
- Blood groups and transfusion
- Eosinophilic Esophagitis
- Immune responses and vaccinations
- Genomics and Rare Diseases
- Circadian rhythm and melatonin
- Crystallization and Solubility Studies
- Endoplasmic Reticulum Stress and Disease
- Bone health and treatments
- Systemic Lupus Erythematosus Research
- Inflammatory Myopathies and Dermatomyositis
- Health Sciences Research and Education
- Medical Imaging and Pathology Studies
- Celiac Disease Research and Management
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2017-2025
Arkansas Children's Hospital
2020-2025
University of Évora
2025
Universidade do Porto
2016-2024
Palmetto Hematology Oncology
2022
ERN BOND
2022
University of Coimbra
2020-2021
University of Aveiro
2011-2016
Universidade Lusófona
2012
We present an experimental NMR, X-ray diffraction (XRD), and computational study of the supramolecular assemblies two crystalline forms Ciprofloxacin: one anhydrate hydrate forming water wormholes. The resonance assignment up to 51 54 distinct (13)C (1)H resonances for is reported. effect crystal packing, identified by XRD, on chemical shifts including weak interionic H-bonds, quantified; shift changes ∼-3.5 ppm CH···π contacts ∼+2 (CH···O((-))); ∼+4.7 (((+))NH···O((-))) H-bonds. Water...
Significance Our findings demonstrate that metabolic supplementation of mucosal T cells, isolated from patients with active ulcerative colitis (UC), N -acetylglucosamine (GlcNAc) leads to the enhancement branched N-glycosylation on cell receptor, which was associated control activation and function. These results were validated in “glycoengineered” mouse models severe colitis. Overall, our open new avenues for a targeted-specific therapy inflammatory bowel disease (IBD). The therapeutic use...
Age-associated mitochondrial dysfunction and oxidative damage are primary causes for multiple health problems including sarcopenia cardiovascular disease (CVD). Though the role of Nrf2, a transcription factor that regulates cytoprotective gene expression, in myopathy remains poorly defined, it has shown beneficial properties both CVD. Sulforaphane (SFN), natural compound Nrf2-related activator genes, provides protection several states CVD is various stages clinical trials, from cancer...
The nature of the immune responses associated with COVID-19 pathogenesis and disease severity, as well breadth vaccine coverage duration immunity, is still unclear. Given unpredictability for developing a severe/complicated disease, there an urgent need in field predictive biomarkers COVID-19. We have analyzed IgG Fc N-glycan traits 82 SARS-CoV-2+ unvaccinated patients, at diagnosis, by nano-LC-ESI-MS. determined impact glyco-variations induction NK cells activation, further evaluating...
Autoimmune diseases are life-threatening disorders that cause increasing disability over time. Systemic lupus erythematosus (SLE) and other autoimmune arise when immune stimuli override mechanisms of self-tolerance. Accumulating evidence has demonstrated protein glycosylation is substantially altered in disease development, but the by which glycans trigger these autoreactive responses still largely unclear. In this study, we found presence microbial-associated mannose structures at surface...
Abstract Inflammatory bowel disease (IBD) is characterized by chronic inflammation in the gut. There growing evidence Crohn’s (CD) of existence a preclinical period immunological changes preceding symptom onset that starts years before diagnosis. Gaining insight into this phase will allow prediction and prevention. Analysis serum samples, up to 6 IBD diagnosis (from PREDICTS cohort), revealed identification unique glycosylation signature on circulating antibodies (IgGs) lower galactosylation...
The perturbation of the symbiotic relationship between microbes and intestinal immune system contributes to gut inflammation Inflammatory Bowel Disease (IBD) development. host mucosa glycans (glycocalyx) creates a major biological interface microorganisms immunity that remains ill-defined. Glycans are essential players in IBD immunopathogenesis, even years before disease onset. However, how changes glycosylation shape microbiome this impact response be clarified. Here, we revealed...
Anti-granulocyte macrophage-colony stimulating factor autoantibodies (aGMAbs) are detected in patients with ileal Crohn's disease (CD). Their induction and mode of action during or before not well understood. We aimed to investigate the underlying mechanisms associated aGMAb induction, from functional orientation recognized epitopes, for their impact on intestinal immune homeostasis use as a predictive biomarker complicated CD. characterized using enzyme-linked immunosorbent assay naturally...
Abstract T-cell development ensures the formation of diverse repertoires receptors (TCRs) that recognize a variety antigens. Glycosylation is major posttranslational modification present in virtually all cells, including T-lymphocytes, regulates activity/functions. Although these structures are known to be involved TCR-selection DP thymocytes, it unclear how glycans regulate other thymic processes and they influence susceptibility disease. Here, we discovered stage-specific glycome...
Changes in protein glycosylation are a hallmark of immune-mediated diseases. Glycans master regulators the inflammatory response and important molecules self-nonself discrimination. This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular identify unique glycosignature that characterizes LN pathogenesis.A comprehensive tissue glycomics characterization performed kidney specimens from patients with systemic erythematosus biopsy-proven LN. A combination...
<title>Abstract</title> <bold>Background. </bold>Achondroplasia is one of the most common skeletal dysplasias, characterized by disproportionate short stature and delayed motor development. Additional potential complications include bowed legs hypotonia, otitis media, sleep apnea, from spinal stenosis, which may impose burden to individuals with achondroplasia, as well their parents family. No reliable, validated publicly available achondroplasia-specific patient-reported outcome (PRO)...
This paper explores the educational transformations brought about by social networks and their implications for teaching research in higher education. Based on a critical analysis of interaction between students teachers platforms, study seeks to understand how these digital tools influence learning, collaboration knowledge construction processes. In addition, investigates challenges opportunities that arise with use academic environment, proposing strategies integrate them meaningful way...
Abstract Transition care (TC) is crucial for young persons with rare bone and mineral conditions (RBMCs) as they move from pediatric to adult healthcare. Effective TC prevents disruptions supports medical psychosocial needs. However, gaps in communication, a shortage of RBMC specialists, challenges navigating healthcare necessitate standardized care. This study aimed develop consensus-based recommendations RBMCs, focusing on best practices seamless transition patient empowerment. A two-round...
Abstract Inflammatory Bowel Disease (IBD) is a chronic inflammatory condition affecting the gastrointestinal tract (GIT). Glucagon‐like peptide‐2 (GLP‐2) analogs possess high potential in treatment of IBD by enhancing intestinal repair and attenuating inflammation. Due to enzymatic degradation poor absorption, GLP‐2 are administered parenterally, which leads patient compliance. This work aims develop IBD‐targeted nanoparticles (NPs) for oral delivery analog, Teduglutide (TED). Leveraging...
ABSTRACT A large variation in the severity of disease symptoms is one key open questions COVID-19 pandemics. The fact that only a small subset people infected with SARS-CoV-2 develop severe suggests there have to be some predisposing factors, but biomarkers reliably predict not been found so far. Since overactivation immune system implicated form and IgG glycosylation known involved regulation different processes, we evaluated association inter-individual N-glycome composition COVID-19....
Introduction: Pneumothorax occurs more frequently in the neonatal period than any other of life and is associated with increased mortality morbidity. Several risk factors for pneumothorax, including respiratory pathology, invasive non-invasive support, predictors have been described.Objective: To evaluate prevalence to assess describe clinical characteristics, management outcome newborn infants as well identify these newborns.Methods: This retrospective case-control study included all...