A5027897950- Pluripotent Stem Cells Research
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Protease and Inhibitor Mechanisms
- Connective tissue disorders research
- Diamond and Carbon-based Materials Research
- Geophysical Methods and Applications
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bone and Dental Protein Studies
- Energy Harvesting in Wireless Networks
- Galectins and Cancer Biology
- Luminescence and Fluorescent Materials
- Graphene and Nanomaterials Applications
- Metal and Thin Film Mechanics
- Advanced biosensing and bioanalysis techniques
- Soil Moisture and Remote Sensing
- Analytical Chemistry and Sensors
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Viral Infections and Vectors
- Sperm and Testicular Function
Chang Gung Memorial Hospital
2017-2022
Linkou Chang Gung Memorial Hospital
2020
Chang Gung University
2020
National Yang Ming Chiao Tung University
2013-2019
Institute of Medical Sciences
2009
In this study, we report a serum-free culture system for primary neonatal pulmonary cells that can support the growth of octamer-binding transcription factor 4+ (Oct-4+) epithelial colonies with surrounding mesenchymal stroma. addition to Oct-4, these also express other stem cell markers such as stage-specific embryonic antigen 1 (SSEA-1), (Sca-1), and Clara secretion protein (CCSP) but not c-Kit, CD34, p63, indicating they represent subpopulation have been implicated lung stem/progenitor in...
Recent studies indicate that neonatal spermatogonial stem cells (SSCs) possess pluripotency. However, the mechanisms regulate pluripotent differentiation capacity of SSCs remain unclear. Here, we describe a new method to clonally derive from mouse testis. By coculturing with testicular stromal cells, can be maintained and expanded in serum-free conditions. Unlike endogenous SSCs, these vitro showed strong alkaline phosphatase (AP) activity displayed characteristics embryonic primordial germ...
This study examines the relationship between power distance and compulsive perception of citizenship behavior from perspective social exchange theory, with supervisor-subordinate guanxi as mediating effect workplace climate moderating effect. A quantitative research method was used to collect data through a questionnaire survey, target population employees various industrial enterprises in Taiwan. found that significantly related behavior, indicating is not only affected by distance, but...
Abstract Stem cell surface markers may facilitate a better understanding of stem biology through molecular function studies or serve as tools to monitor the differentiation status and behavior cells in culture tissue. Thus, it is important identify additional novel markers. We used glycoproteomics discover glycoproteins on human embryonic (hESCs) that be useful found glycoprotein, leucine-rich repeat neuronal protein 1 (LRRN1), expressed abundantly hESCs before into embryoid bodies (EBs)....
The lung is a radiosensitive organ, which imposes limits on the therapeutic dose in thoracic radiotherapy. Irradiated alveolar epithelial cells promote radiation-related pneumonitis and fibrosis. However, role of stem (LSCs) development radiation-induced injury still unclear. In this study, we found that both LSCs LSC-derived type II (AECII) can repair DNA double-strand breaks, but irradiated underwent growth arrest cell differentiation faster than AECII cells. Moreover, radiation drove to...
Abstract Chronic obstructive pulmonary disease (COPD) is a progressive lung with high morbidity and mortality worldwide. Although several mechanisms to account for deleterious immune effects were proposed, molecular description the underlying alveolar structural alterations COPD lacking. Here, silencing of α1,6-fucosyltransferase (Fut8), enzyme core-fucosylation highly expressed in stem cells, resulted changes organoids, recapitulating COPD. Site-specific mass spectrometry analysis...
Time-domain reflectometry (TDR) has been a powerful tool for measuring soil dielectric properties. Initiating from apparent constant ( K ) measurement up until and complex spectroscopies, the embedded information in TDR signal can be extracted to inspire our understanding of underlying behaviors. Multiple full waveform inversion techniques have developed extract permittivity (CDP) spectrum, but most them involved prior knowledge input function tedious calibration. This rendered field...