A5079705346- Extracellular vesicles in disease
- HIV Research and Treatment
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Advanced Proteomics Techniques and Applications
- interferon and immune responses
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Environmental Toxicology and Ecotoxicology
- DNA and Nucleic Acid Chemistry
- Gestational Diabetes Research and Management
- Protease and Inhibitor Mechanisms
- Sleep and related disorders
- Machine Learning in Bioinformatics
- Pregnancy and preeclampsia studies
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Selenium in Biological Systems
Jinan University
2012-2023
Beijing Technology and Business University
2023
Quzhou University
2023
University of Electronic Science and Technology of China
2023
First Affiliated Hospital of Jinan University
2023
Heilongjiang University
2021
As a well-known phenomenon, total mRNAs poorly correlate to proteins in their abundances as reported. Recent findings calculated with bivariate models suggested even poorer such correlation, whereas focusing on the translating (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) subset. In this study, we analysed relative of RNC-mRNAs and genome-wide scale, comparing human lung cancer A549 H1299 cells normal bronchial epithelial (HBE) cells, respectively. discovered, strong correlation...
Abstract Size‐exclusion chromatography (SEC) is a widely adopted method for the isolation of extracellular vesicles (EVs) from complex samples. SEC can efficiently remove high‐abundant proteins, while often requires multiple fractionation operation using diversified column settings. In this study, we aim to establish simplified acquire high quality EVs. comparison all three cross‐linked Sepharose resins with sample types FBS and human serum (HS), CL‐6B CL‐4B showed superior performance in...
The exosome is a key initiator of pre-metastatic niche in numerous cancers, where macrophages serve as primary inducers tumor microenvironment. However, the proteome that can be exosomally transported from cancer cells to has not been sufficiently characterized so far. Here, we used colorectal (CRC) exosomes educate tumor-favorable macrophages. With SILAC-based mass spectrometry strategy, successfully traced CRC Such primarily focused on promoting cytoskeleton rearrangement, which was...
In the process of translation, ribosomes first assemble on mRNAs (translation initiation) and then translate along mRNA (elongation) to synthesize proteins. Elongation pausing is deemed highly relevant co-translational folding nascent peptides functionality protein products, which positioned evaluation elongation speed as one central questions in field translational control. By integrating three types RNA-seq methods, we experimentally computationally resolved speed, with our proposed...
Abstract Extracellular vesicles (EVs) and their clinical applications have been recognized as one of the most fast‐growing promising fields. Numerous groups including us expressed concerns on idea isolating “pure” EVs for different purposes, there are no specific markers to date that can examine exact EV purity. Typically, major instrument need yielding loss primary compensations getting with higher As such, especially in biomarker‐based tests, ceaseless efforts obtaining more pure worth...
Exosomes are deliverers of critically functional proteins, capable transforming target cells in numerous cancers, including hepatocellular carcinoma (HCC). We hypothesize that the motility HCC can be featured by comparative proteome exosomes. Hence, we performed super-SILAC-based MS analysis on exosomes secreted three human cell lines, non-motile Hep3B cell, and motile 97H LM3 cells. More than 1400 exosomal proteins were confidently quantified each with highly biological reproducibility....
Chromosome-centric human proteome project (C-HPP) aims at differentiating chromosome-based and tissue-specific protein compositions in terms of expression, quantification, modification. We previously found that the analysis translating mRNA (mRNA attached to ribosome-nascent chain complex, RNC-mRNA) can explain over 94% mRNA-protein abundance. Therefore, we propose here use full-length RNC-mRNA information illustrate expression both qualitatively quantitatively. performed RNA-seq on...
Identification of all phosphorylation forms known proteins is a major goal the Chromosome-Centric Human Proteome Project (C-HPP). Recent studies have found that certain phosphoproteins can be encapsulated in exosomes and function as key regulators tumor microenvironment, but no deep coverage phosphoproteome human has been reported to date, which makes exosome potential source for new phosphosite discovery. In this study, we performed highly optimized MS analyses on exosomal cellular isolated...
Nasopharyngeal carcinoma (NPC) is a serious threat to public health, and the biomarker discovery of urgent needs. The data-independent mode (DIA) based sequential window acquisition all theoretical fragment-ion spectra (SWATH) mass spectrometry (MS) has been proved be precise in protein quantitation efficient for cancer researches. In this study, we performed first SWATH-MS analysis comparing NPC normal tissues. Spike-in stable isotope labeling by amino acids cell culture (super-SILAC) MS...
Preeclampsia (PE) is a placenta disease, featured by hypertension, proteinuria, and other multiorgan dysfunctions, its etiology unclear. We others have shown that intensive endoplasmic reticulum (ER) stress unfolded protein response (UPR) occur in the PE placenta. In this study, we isolated detergent-insoluble proteins (DIPs) from human tissues, which were enriched with aggregates, to characterize UPR PE. With data-independent acquisition (DIA) mass spectrometry, identified 2066 DIPs across...
Our first proteomic exploration of human chromosome 1 began in 2012 (CCPD 1.0), and the genome-wide characterization proteome through public resources revealed that 32–39% proteins on remain unidentified. To characterize all missing proteins, we applied an OMICS-integrated analysis three liver cell lines (Hep3B, MHCC97H, HCCLM3) using mRNA ribosome nascent-chain complex-bound deep sequencing profiling, contributing mass spectrometric evidence 60 additional gene products. Integration...
Abstract Synovitis is a key contributor to the inflammatory environment in osteoarthritis (OA) joints. Currently, biological therapy of OA not satisfactory multiple single-target trials on anti-TNF agents, or IL-1 antagonists. Systems understanding phosphorylation state synovium warranted direct further therapeutic strategies. Therefore, this study, we compared human synovial phosphoproteome with acute joint fracture subjects. We found that had significantly more phosphoproteins, and 82...
Abstract G-quadruplex (G4), a non-classical deoxyribonucleic acid structure, is widely distributed in the genome and involved various biological processes. In vivo, high-throughput sequencing has indicated that G4s are significantly enriched at functional regions cell-type-specific manner. Therefore, prediction of based on computational methods necessary instead time-consuming laborious experimental methods. Recently, G4 CUT&Tag been developed to generate higher-resolution data than...
Organoselenium have garnered attention because of their potential to be used as ingredients in new anti-aging and antioxidation medicines food. Rotifers are frequently a model organism for aging research. In this study, we Se-enriched Chlorella (Se- Chlorella), novel organoselenium compound, feed Brachionus plicatilis establish rotifer with prolonged lifespan. The results showed that the antioxidative effect was associated an increase guaiacol peroxidase (GPX) catalase (CAT). authors then...
Factors predicting peripheral blood total HIV-1 DNA size in chronically infected patients with successfully suppressed viremia remain unclear. Prognostic power of such factors are clinical significance for making decisions.Two sets study populations were included: 490 China AIDS Clinical Trial (CACT) participants (Training cohort, followed up 144 to 288 weeks) and 117 outpatients from Peking Union Medical College Hospital (PUMCH) (Validation more than 96 weeks). All HIV-1-infected achieved...
Abstract Prolonged antiretroviral therapy (ART)-free remission post-treatment has been observed and reported in human immunodeficiency virus-1 (HIV-1) infection. The primary factors for such achievement have linked to the transcriptional inactivation of HIV-1 DNA immune recovery. Here, we a patient with acute infection who immediately received intensified quadruple ART. At month 9 (M9), an old CFDA-approved immuno-suppressive herbal medicine, Tripterygium Wilfordii Hook F (TwHF), was used...
Abstract Background: Factors predicting peripheral blood total HIV-1 DNA size in chronically infected patients with successfully suppressed viremia remain unclear. Prognostic power of such factors are clinical significance for making decisions. Methods: Total at baseline, 12, 24, 48, 96, and 288 weeks after combined antiretroviral therapy (cART) initiation 607 treatment-naïve chronic infection were quantified. Generalized estimating equations logistic regression methods used to derive...
Abstract Background: Factors predicting peripheral blood total HIV-1 DNA size in chronically infected patients with successfully suppressed viremia remain unclear. Prognostic power of such factors are clinical significance for making decisions. Methods: Total at baseline, 12, 24, 48, 96, and 288 weeks after combined antiretroviral therapy (cART) initiation 607 treatment-naïve chronic infection were quantified. Generalized estimating equations logistic regression methods used to derive...
To evaluate the clinical effect and effectiveness of acupuncture therapy in treatment insomnia patients, so as to provide better evidence based services, benefit patients promote it practice. Chinese databases were searched: China National Knowledge Infrastructure, Scientific Journals Full text Database, Wanfang data knowledge service platform; English PubMed, Cochrane Library, Embase. Stata 15.1 RevMan 5.4.1 software used for statistical analysis. A total 22 randomized controlled trials...