A5033715748- Circular RNAs in diseases
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Pancreatitis Pathology and Treatment
- MicroRNA in disease regulation
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Cancer Mechanisms and Therapy
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immunotherapy and Immune Responses
- FOXO transcription factor regulation
- Gallbladder and Bile Duct Disorders
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
Nanjing Medical University
2019-2025
Jiangsu Province Hospital
2019-2023
Abstract Background A growing number of studies have focused on investigating circRNAs as crucial regulators in the progression multiple cancer types. Nevertheless, biological effects and underlying mechanisms pancreatic ductal adenocarcinoma (PDAC) remain unclear. Methods Differentially expressed between cancerous tissue adjacent normal tissues were identified by RNA sequencing PDAC. Subsequently, vitro vivo functional experiments performed to investigate roles circNEIL3 PDAC tumour growth...
Circular RNAs (circRNAs) are becoming a unique member of non-coding (ncRNAs) with emerging evidence their regulatory roles in various cancers. However, regards to pancreatic ductal adenocarcinoma (PDAC), circRNAs biological functions remain largely unknown and worth investigation for potential therapeutic innovation.In our previous study, next-generation sequencing was used identify differentially expressed 3 pairs PDAC adjacent normal tissues. Further validation circRHOBTB3 expression...
Pancreatic ductal adenocarcinoma (PDAC) has a poor prognosis and high mortality rate. The transcription factor YY1 acts as an inhibitor of many types tumors. We found that knockdown promoted the invasion migration PANC-1 BxPC-3 cells; FER partially restored promotion pancreatic cancer caused by knockdown. In vivo experiments yielded same results. According to luciferase reporter gene, electrophoretic mobility shift (EMSA) chromatin immunoprecipitation (ChIP) assays, directly binds promoter...
Pancreatic cancer (PDAC) is a highly invasive with poor prognosis. Recent research has found that the transcription factor Yin Yang 1 (YY1) plays an inhibitory role in development of pancreatic cancer. It been reported tubulin polymerisation-promoting protein (TPPP) indispensable variety tumours, but its expression and have not yet elucidated.In this study, we performed ChIP-sequencing YY1 directly binds to promoter region TPPP. The TPPP was detected by western blotting immunohistochemistry....
Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant tumors has worst prognoses, and role long noncoding RNAs (lncRNAs) in biological pathological processes pancreatic cancer, including tumor cell proliferation, a popular topic research. Our previous study revealed correlation between high levels lncRNA‐SOX2OT (SOX2OT) with poor survival outcomes. Cell Counting Kit‐8, EdU, Flow cytometry Colony formation assays as well Xenograft growth PDAC cells mice were used for detection...
Abstract Pancreatic cancer (PC) is a malignant tumor with poor prognosis and high mortality. However, the biological role of miR-548t-5p in PC has not been reported. In this study, we found that expression was significantly decreased tissues compared adjacent tissues, low associated behavior. addition, inhibited proliferation, migration, invasion cell lines. Regarding molecular mechanism, luciferase reporter gene, chromatin immunoprecipitation (ChIP), functional recovery assays revealed YY1...
Pancreatic cancer (PC) has been becoming a common with high mortality and quantitative real-time polymerase chain reaction (qPCR) is one of the best choices for researching gene expression. Internal reference genes, such as actin beta (ACTB) glyceraldehyde-3-phosphatide hydrogenase (GAPDH) have long used in relative quantification analysis. But evidence shows that some internal genes expression may vary different tissues, cell lines conditions. The present study aimed to find more stable...
Objective: To compare the short-term outcomes and long-term survivals of radical antegrade modular pancreatosplenectomy(RAMPS) conventional distal pancreatectomy(CDP). Methods: A total consecutive 304 patients including 176 male 128 female who underwent RAMPS or CDP at Pancreas Center, First Affiliated Hospital with Nanjing Medical University from May 2013 to June 2019 were retrospectively analyzed. The median age was 64.1 years old (range:39 85 old). There 101 203 CDP. Measurement data...
Recurrence of liver metastasis after pancreatectomy is often a predictor poor prognosis. Comprehensive genomic analysis may contribute to better understanding the molecular mechanisms postoperative and provide new therapeutic targets.A total 67 patients from The Cancer Genome Atlas (TCGA) were included in this study. We analyzed differentially expressed genes (DEGs) by R package "DESeq2." Weighted gene co-expression network (WGCNA) was applied investigate key modules hub genes....
Objective: To investigate the clinical efficacy of distal pancreatectomy with celiac axis resection(DP-CAR). Methods: A total 89 consecutive patients (50 males and 39 females) who were diagnosed pancreatic body cancer underwent DP-CAR in Pancreas Center,First Affiliated Hospital Nanjing Medical University between September 2013 June 2022 retrospectively reviewed. There 50 females,with age(M(IQR)) 63(12) years(range:43 to 81 years). Perioperative parameters,pathology results follow-up data...
Abstract Background LncRNAs are reported to play an essential role in multiple tumors, including pancreatic cancer. could impact tumor growth via RNA-binding proteins, working as a coactivators of transcription factors or impacting their gene expression posttranscriptional regulation. Our study aimed elucidate the function and mechanism lncRNA KCNK15-AS1 its binding protein ACTR3B PC progression. previous data indicated that is downregulated tissues cell lines compared normal controls....
Objective: To compare the outcomes of modified Appleby procedure and sub-adventitial divestment technique for locally advanced or borderline resectable pancreatic body cancer. Methods: A total consecutive 58 patients(33 males 25 females) who were diagnosed as cancer underwent distal pancreatectomy at Pancreas Center, First Affiliated Hospital Nanjing Medical University between September 2013 May 2019 retrospectively reviewed. The age(M(IQR)) was 62(9)years(range: 43 to 79 years). Thirty-one...
Objective: To evaluate the value of left-sided uncinate process first approach in pancreaticoduodenectomy. Methods: The clinical data 152 patients who underwent during pancreaticoduodenectomy at Pancreas Center, First Affiliated Hospital Nanjing Medical University from January 2020 to December were analyzed retrospectively. There 64 females and 88 males,with age(M(QR)) 62.0(14.7)years(range:16.0 84.0 years). date 117 without using same period was selected as control group,including 65 52 age...