A5043134223- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Food Safety and Hygiene
- Listeria monocytogenes in Food Safety
- Aquaculture disease management and microbiota
- Pharmaceutical and Antibiotic Environmental Impacts
- Identification and Quantification in Food
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Trace Elements in Health
- Protein Degradation and Inhibitors
- Multiple Myeloma Research and Treatments
- Food Industry and Aquatic Biology
- Education and Digital Technologies
- Educational Practices and Policies
- Histone Deacetylase Inhibitors Research
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Cancer Research and Treatments
- Enterobacteriaceae and Cronobacter Research
- Ubiquitin and proteasome pathways
- Education Pedagogy and Practices
- Oral microbiology and periodontitis research
- Viral gastroenteritis research and epidemiology
- Urinary Tract Infections Management
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2021-2024
National Institute of Engineering, Technology and Innovation
2023
University of Trás-os-Montes and Alto Douro
2023
Instituto Nacional de Engenharia Biomédica
2023
Hospital Pedro Hispano
2023
Administración Nacional de Laboratorios e Institutos de Salud
2022
Universidade do Porto
2013-2021
Rede de Química e Tecnologia
2013-2021
University of Glasgow
2013-2021
Queen Mary University of London
2019
Highlights•YTHDF2 is highly expressed across human AML and essential for leukemia initiation•YTHDF2 shortens the half-life of m6A-modified transcripts in AML•Loss YTHDF2 expands HSCs but does not derail hematopoiesis•YTHDF2 protects cells from apoptosis by downregulating TNFR2SummaryAcute myeloid (AML) an aggressive clonal disorder hematopoietic stem (HSCs) primitive progenitors that blocks their differentiation, generating self-renewing leukemic (LSCs). Here, we show mRNA m6A reader...
The mcr-1 gene was found in 11 isolates from a Portuguese Salmonella collection (n = 1,010; 58 serotypes; 2002–15) of clinical samples, foodstuff, food-animals and water. Mcr-1 has been located on different plasmids (IncX4/IncHI2) pig-associated multidrug-resistant, copper-tolerant S. 1,4,[5],12:i:-/ST34 S . Rissen/ST469 clones human pork products since at least 2011. Our data highlight dissemination by successful resistant Europe raise questions about the efficacy copper-based interventions...
Abstract Acute myeloid leukemia (AML) is a typically lethal molecularly heterogeneous disease, with few broad-spectrum therapeutic targets. Unusually, most AML retain wild-type TP53 , encoding the pro-apoptotic tumor suppressor p53. MDM2 inhibitors (MDM2i), which activate p53, and BET (BETi), targeting BET-family co-activator BRD4, both show encouraging pre-clinical activity, but limited clinical activity as single agents. Here, we report enhanced toxicity of combined MDM2i BETi towards cell...
We studied the occurrence of diverse copper (Cu) tolerance genes from Gram-positive bacteria and their co-transfer with antibiotic resistance among Enterococcus sources. (n = 922) several species human, animal, environment food samples were included. Antimicrobial CuSO4 susceptibility conjugation assays performed by standard procedures, bacterial screening Cu PCR, clonality PFGE/multilocus sequence typing. tcrB cueO occurred in 15% 137/922) 14% 128/922) isolates, respectively, highest...
We describe a hospital-based outbreak caused by multidrug-resistant, Klebsiella pneumoniae carbapenemase 3-producing, mcr-1-positive K. sequence type 45 in Portugal. mcr-1 was located an IncX4 plasmid. Our data highlight the urgent need for systematic surveillance of to support adequate therapeutic choices nosocomial setting.
Vascular calcification (VC) is a frequent condition in chronic kidney disease (CKD) and well-established risk factor for the development of cardiovascular (CVD). Gut dysbiosis may contribute to CVD inflammation CKD patients. Nonetheless, role gut blood microbiomes CKD-associated VC remains unknown. Therefore, this pilot study aimed explore link between patients on peritoneal dialysis (CKD-PD). Our results showed relative changes specific taxa CKD-PD with without VC, namely Coprobacter,...
Factors driving the expansion of particular MDR Salmonella serotypes/clones are not completely understood. We assessed if emergent were more enriched in metal tolerance genes (e.g. to Cu/Ag) than other less frequent ones, as an additional feature survive environments contaminated with metals. Metal (Cu pco/Ag,Cu sil/Hg mer/As ars/Te ter) screening (PCR/sequencing), MICs CuSO4/AgNO3 (aerobiosis/anaerobiosis), genetic element characterization (S1/I-CeuI PFGE) and conjugation assays performed a...
Staphylococcus aureus is both a human commensal and pathogen, that causes serious nosocomial community-acquired infections. Despite nostrils being considered its preferred host habitat, the oral cavity has been demonstrated to be an ideal starting point for auto-infection transmission. The antibiotic resistance assessment of S. priority often reported in clinical settings. This study aimed explore prevalence antimicrobial susceptibility nasal cavities healthy individuals. participants (n =...
Lentinula edodes, commonly known as shiitake mushroom, is renowned for its potential health advantages. This research delves into the often-overlooked by-product of cultivation, namely spent mushroom substrate (SMS), to explore nutraceutical properties. The SMS samples were collected and subjected different extraction methods, short or long agitation, ultrasound-assisted extractions using temperatures distilled water a 50% (v/v) ethanol solvents. extracts tested phenolic content (total...
A Terapia Transpessoal Sistêmica tem como característica em seu método a localização de conflitos. Neste trabalho relata-se um caso clínico, no qual o uso da apresentou rápida e eficiente evolução uma paciente que apresentava queixas ausência do pai, baixa energia vitalidade. partir das primeiras sessões se encontrava mais feliz animada, conseguiu fazer Yoga Meditação, segundo mesma estas atividades auxiliava com TDAH. Após aplicação técnica Carta Âncora Solo, relatou recebeu ligação pai....
Expansion of mcr-carrying Enterobacteriaceae (MCR-E) is a well-recognized problem affecting animals, humans and the environment. Ongoing global control actions involve colistin restrictions among food-animal production, but their impact on poultry-derived products largely unknown, justifying comprehensive farm-to-fork studies. Occurrence MCR-E 53 chicken-meat batches supplied from 29 Portuguese farms shortly after withdrawal was evaluated. Strains (FT-IR/MLST/WGS), mcr plasmids adaptive...
Yeast acquisition begins at birth; however, the contribution of mother on yeast transmission to offspring and associated resistance is yet be clarified. The aim this study was explore vertical yeasts their antifungal susceptibility profile in early life. Oral, fecal, breastmilk samples were collected from 73 mother–child pairs four twelve weeks after delivery cultured Sabouraud dextrose agar with chloramphenicol. isolates identified by MALDI-TOF MS. studied microsatellite genotyping....