A5082058900- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Transplantation Techniques and Outcomes
- Pancreatitis Pathology and Treatment
- Gallbladder and Bile Duct Disorders
- Neuroendocrine Tumor Research Advances
- Gastric Cancer Management and Outcomes
- Renal Transplantation Outcomes and Treatments
- Liver Disease and Transplantation
- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Multiple and Secondary Primary Cancers
- Pediatric Hepatobiliary Diseases and Treatments
- Gastrointestinal Tumor Research and Treatment
- Renal cell carcinoma treatment
- Esophageal and GI Pathology
- Metastasis and carcinoma case studies
- Pancreatic function and diabetes
- Genetic factors in colorectal cancer
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Cancer Mechanisms and Therapy
Osaka University
2016-2025
Toyonaka Municipal Hospital
2016-2024
Japan Pancreatic Disease Research Foundation
2021-2023
The Japanese Society of Gastroenterological Surgery
2023
Osaka International Cancer Institute
2005-2022
Osaka University Hospital
2019-2021
Kyushu University
2020
Brown University
2012-2019
Rhode Island Hospital
2012-2019
American Gastroenterological Association
2016
Abstract The cancer drug gemcitabine (GEM) is a key for treating pancreatic ductal adenocarcinoma (PDAC), but PDAC cells develop chemoresistance after long-term administration. Since the tolerance was immediately spread to every tissue in patient, it assumed that some certain efficient mechanisms underlay development of chemoresistance. Changes levels particular microRNAs or alterations intercellular communication play dominant role development, and recent data also suggest exosomes an...
We investigated the potential of adipose-derived stem cells (ADSCs) in preventing post-hepatectomy liver failure, emphasizing necessity direct administration using a scaffold. A fibrin gel scaffold was employed for ADSCs (gelADSC) to assess their therapeutic impact on regeneration both vitro and vivo settings. Experiments were conducted C57BL/6 mice with normal livers those chronic hepatitis. also explored role extracellular vesicles (EVs) secreted by conjunction gel. GelADSC showed...
We reported recently the clinical efficiency of interferon (IFN)-α/5-fluorouracil (5-FU) combination therapy in advanced hepatocellular carcinoma (HCC). However, prediction response to remains unsatisfactory. The aim this study was investigate anti-tumour effects microRNA (miR)-21 on sensitivity HCC cells IFN-α/5-FU and whether miR-21 can be used as a predictor such HCC.Changes (PLC/PRF/5 HepG2) were examined after transfection with pre-miR-21 or anti-miR-21. correlation between expression...
The tumor suppressor p53 has been implicated in the pathogenesis of non-cancer-related conditions such as insulin resistance, cardiac failure, and early aging. In addition, accumulation observed hepatocytes individuals with fibrotic liver diseases, but significance this is not known. Herein, we have mechanistically linked activation to fibrosis. Hepatocyte-specific deletion mice gene encoding Mdm2, a protein that promotes degradation, led hepatocyte synthesis connective tissue growth factor...
Pancreatic cancer has a poor prognosis because of its high refractoriness to chemotherapy and tumour recurrence, these properties have been attributed stem cells (CSCs). MicroRNA (miRNA) regulates various molecular mechanisms progression associated with CSCs. This study aimed identify the candidate miRNA characterise clinical significance. We established gemcitabine-resistant Panc1 cells, induced CSC-like through sphere formation. Candidate miRNAs were selected microarray analysis. The...
Abstract Angiogenesis is a critical step in the development and progression of hepatocellular carcinoma (HCC). Myeloid lineage cells, such as macrophages monocytes, have been reported to regulate angiogenesis mouse tumor models. TIE2, receptor angiopoietins, conveys pro-angiogenic signals identifies monocyte/macrophage subset with activity. Here, we analyzed occurrence kinetics TIE2-expressing monocytes/macrophages (TEMs) HCC patients. This study enrolled 168 HCV-infected patients including...
Gemcitabine-based chemotherapy is the standard treatment for pancreatic cancer. However, issue of resistance remains unresolved. The aim this study was to identify microRNAs (miRNAs) that govern gemcitabine in cancer.miRNA microarray analysis using gemcitabine-resistant clones MiaPaCa2 (MiaPaCa2-RGs), PSN1 (PSN1-RGs), and their parental cells (MiaPaCa2-P, PSN1-P) conducted. Changes anti-cancer effects were studied after gain/loss-of-function candidate miRNA. Further assessment putative...
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death. High recurrence rates after curative resection and lack specific biomarkers for intrahepatic metastases are major clinical problems. Recently, exosomal microRNAs (miRNAs) have been reported to a role in formation pre-metastatic niche as promising patients with malignancy. Here we aimed clarify molecular mechanisms metastasis identify novel biomarker miRNA HCC. A highly metastatic cell line (HuH-7M) was...
Although we studied previously the mechanisms of resistance pancreatic cancer cells to gemcitabine (GEM), prediction response GEM remains unsatisfactory. The aim this study was investigate relationship between miR-29a expression and in cells. Changes growth-inhibitory effect (MIAPaCa-2, PSN-1, BxPC-3 Panc-1) were examined after overexpression or suppression miR-29a. We also on Wnt/β-catenin signaling pathway investigated whether altered by weakened addition Wnt3a, a activator. MIAPaCa-2...
Single-cell RNA sequencing (scRNAseq) has been used to assess the intra-tumor heterogeneity and microenvironment of pancreatic ductal adenocarcinoma (PDAC). However, previous knowledge is not fully universalized. Here, we built a single cell atlas PDAC from six datasets containing over 70 samples >130,000 cells, demonstrated its application reanalysis bulk transcriptomic cohorts inferring cell-cell communications. The decomposition transcriptomics using scRNAseq data showed cellular PDAC;...
Tumor endothelial cells (TECs) promote tumor angiogenesis and regulate cytotoxic T in the microenvironment. However, roles of TECs for tumor-infiltrating T-cell hepatocellular carcinoma (HCC) is still unknown. Here, we aimed to investigate how influenced growth immune responses HCC focusing on CD8+ infiltration exhaustion. First, were isolated from subcutaneous tumors with murine cell lines (BNL-T) magnetic selection CD31+ cells, normal (NECs) liver. Second, immunocompetent mice injected...
Immune checkpoint inhibitors (ICIs) could cause type 1 diabetes (T1D). However, the underlying mechanism remains unclear. We immunohistochemically analyzed pancreatic specimens from three individuals with ICI-related T1D, and their histopathological data were compared those patients who had received ICI therapy but did not develop T1D (non-T1D) seven normal glucose-tolerant subjects as control subjects. All susceptible HLA haplotypes. In β-cell area decreased α-cell increased non-T1D The...
Background and Objectives Although salvage surgery after definitive chemoradiotherapy (CRT) is common, the safety indication has not yet been established. Methods We retrospectively compared mortality morbidity of 24 patients who underwent with those historical controls treated neoadjuvant CRT followed by planned esophagectomy during same period, analyzed prognostic factor surgery. Results Preoperative serum albumin (3.7 vs. 4.1 g/dl, P = 0.0157) lymphocyte count (763 964/mm3, 0.0111) in...
Metabolism may determine the biologically malignant behavior of pancreatic cancer. To investigate significance and prognostic value cancer metabolism in patients, we investigated expression two key enzymes anaerobic glycolysis, hexokinase 2 (HK2) pyruvate kinase isoenzyme type M2 (PKM2), surgical specimens obtained from 36 patients who underwent curative resection ductal carcinoma. The hk2-glycolysis axis is a system clinical imaging tumors via positron emission tomography....
Biliary tract cancer ( BTC ) has a generally poor prognosis. Furthermore, it is difficult to distinguish from benign biliary disease BBD with commonly used modalities. Therefore, novel biomarker facilitate detection highly desirable. Recent studies have reported the use of circulating micro RNA s (mi s) as biomarkers for cancers. The purpose this study was evaluate whether mi ‐21 R ‐21) could be . Plasma samples were obtained 94 patients, 50 healthy volunteers HV s), and 23 patients. levels...
Background and Objectives Preoperatively evaluating reserved liver function is critical in preventing posthepatectomy failure (PHLF) patients undergoing resection. The commonly used indocyanine green (ICG) clearance test has several drawbacks. Patients would benefit from a more reliable straightforward means of assessing the risk PHLF. Methods This study included 277 with hepatocellular carcinoma (HCC) predictive value known factors for PHLF was compared to that ICG. Results identified 25...
Epithelial-mesenchymal transition (EMT) is involved in the characteristics of malignancy, such as invasion, metastasis, and chemoresistance. In biliary tract cancer (BTC), EMT induced by transforming growth factor-beta 1 (TGF-β1). The reversible; therefore, it conceivable that could be related to some epigenetic changes. We focused on histone deacetylase (HDAC) inhibitors regulators TGF-β1 signaling, investigated their effect employed four BTC cell lines (MzChA-1, gemcitabine-resistant...