A5033036656- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Nail Diseases and Treatments
- Long-Term Effects of COVID-19
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Mycology
- Neutropenia and Cancer Infections
- Plant Pathogens and Fungal Diseases
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- SARS-CoV-2 detection and testing
- COVID-19 and Mental Health
- Cytomegalovirus and herpesvirus research
- Actinomycetales infections and treatment
- Infectious Diseases and Tuberculosis
- Toxin Mechanisms and Immunotoxins
- Phytoplasmas and Hemiptera pathogens
- Antibiotic Use and Resistance
- COVID-19 diagnosis using AI
- Prostate Cancer Diagnosis and Treatment
- Orthopedic Infections and Treatments
- Sepsis Diagnosis and Treatment
Mahidol University
2016-2025
Siriraj Hospital
2016-2025
Novem (Netherlands)
2022
Francisco Muñoz Irles (Spain)
2022
Westmead Hospital
2008-2011
The University of Sydney
2008-2011
Westmead Institute
2008-2011
Duke Medical Center
2006-2009
Duke University Hospital
2006
Abstract Background The Coronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Hence, there is an urgent need for rapid, simple, and accurate tests diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Performance characteristics of rapid SARS-CoV-2 antigen detection test should be evaluated compared with gold standard real-time reverse transcription-polymerase chain reaction (RT-PCR) diagnosis COVID-19 cases. Methods test, Standard™ Q...
Bloodstream infections caused by Candida species are of increasing importance and associated with significant mortality. We performed a multi-centre prospective observational study to identify the antifungal susceptibilities invasive bloodstream isolates in Asia-Pacific region. The was carried out over two year period, involving 13 centers from Brunei, Philippines, Singapore, South Korea, Taiwan, Thailand, Vietnam. Identification at each center, reconfirmed central laboratory. Susceptibility...
An online survey of mycology laboratories in seven Asian countries was conducted to assess the status, competence, and services available. Country representatives from Asia Fungal Working Group (AFWG) contacted as many performing diagnosis possible their respective countries, requesting that laboratory heads complete survey. In total, 241 responded, including 71 China, 104 India, 11 Indonesia, 26 Philippines, four Singapore, 18 Taiwan, Thailand. Overall, 129/241 (53.5%) surveyed operate...
Ensitrelvir, a severe acute respiratory syndrome coronavirus-2 main protease inhibitor, has demonstrated clinical and virologic efficacy in previous studies. In this global phase 3 trial, nonhospitalized adults with mild-to-moderate coronavirus disease 2019 (COVID-19) symptom onset within 5 days were randomized (1:1) to receive once-daily ensitrelvir (375 mg day 1, 125 2-5) or blinded matching placebo. The primary endpoint was the restricted mean time sustained (≥2 days) resolution of 15...
Secreted phospholipase B1 (CnPlb1) is essential for dissemination of Cryptococcus neoformans to the central nervous system (CNS) yet components its secretion machinery remain be elucidated. Using gene deletion analysis we demonstrate that CnPlb1 dependent on CnSEC14 product, CnSec14-1p. CnSec14-1p a homologue phosphatidylinositol transfer protein ScSec14p, which and viability in Saccharomyces cerevisiae. In contrast CnPlb1, neither laccase 1-induced melanization within cell wall nor capsule...
ABSTRACT The trehalose pathway is essential for stress tolerance and virulence in fungi. We investigated the importance of this pathogenic yeast Cryptococcus gattii using highly virulent Vancouver Island, Canada, outbreak strain R265. Three genes putatively involved biosynthesis, TPS1 (trehalose-6-phosphate [T6P] synthase) TPS2 (T6P phosphatase), degradation, NTH1 (neutral trehalose), were deleted strain, creating R265 tps1 Δ, tps2 nth1 Δ mutants. As neoformans , cellular was reduced...
Cryptococcus neoformans survives host temperature and regulates cell wall integrity via a calcium-dependent phosphatase, calcineurin. However, downstream effectors of C. calcineurin are largely unknown. In S. cerevisiae other fungal species, calcineurin-dependent transcription factor Crz1, translocates to nuclei upon activation triggers expression target genes. We now show that the Crz1 ortholog (Crz1/Sp1), previously identified as protein kinase C during starvation, is bona fide under...
Infection after transrectal prostate biopsy has become an increasing concern due to fluoroquinolone resistant bacteria. We determined whether colonization identified by rectal culture can identify men at high risk for post-transrectal infection.Six institutions provided retrospective data through a standardized, web based entry form on patients undergoing who had performed. The primary outcome was any infection and the secondary hospital admission 30 days biopsy. used chi-square logistic...
Cryptococcal infections are primarily caused by two related fungal species: Cryptococcus neoformans and gattii. It is well known that C. generally affects immunocompromised hosts; however, gattii infection can cause diseases in not only hosts but also immunocompetent individuals. While recent studies suggest could dampen pulmonary neutrophil recruitment inflammatory cytokine production hosts, the impact of on development their adaptive T helper cell immune response has been addressed. Here,...
This study aimed to investigate the risk factors for and outcomes of patients with candidemia caused by non-albicans Candida. Candidemia treated at Siriraj Hospital (Bangkok, Thailand) during January 2016 December 2017 were enrolled. A total 156 (mean age: 65 years, 56.4% male) included. The most prevalent underlying conditions diabetes (32.1%), chronic cardiac disease (28.2%), kidney (26.9%), hematologic malignancies (21.2%). Candida species isolated from patient blood C. tropicalis...
Summary Phospholipase B1 (Plb1) is secreted after release from its glycosylphosphatidylinositol anchor and implicated in initiation dissemination of infection the pathogenic fungus, Cryptococcus neoformans . To investigate role phosphatidylinositol‐specific phospholipase C (PI‐PLC) Plb1 secretion, we identified two putative PI‐PLC‐encoding genes C. var. grubii ( PLC1 PLC2 ), created Δ plc1 plc2 deletion mutants. In , which expressed less PI‐PLC activity than wild type (WT), three major...
Fatty acid synthase in the yeast Cryptococcus neoformans is composed of two subunits encoded by FAS1 and FAS2 genes. We inserted a copper-regulated promoter (P(CTR4-2)) to regulate expression (strains P(CTR4-2)/FAS1 P(CTR4-2)/FAS2, respectively). Both mutants showed growth rates similar those wild type low-copper medium which were expressed, but even presence exogenous fatty acids, strains suppressed under high-copper conditions. The treatment C. with fluconazole was shown have an increased...
ABSTRACT Phospholipase C (PLC) of Cryptococcus neoformans (Cn Plc1 ) is crucial for virulence this fungal pathogen. To investigate the mechanism CnPlc1-mediated signaling, we established that phosphatidylinositol 4,5-bisphosphate (PIP 2 a major CnPlc1 substrate, which hydrolyzed to produce inositol trisphosphate (IP 3 ). In Saccharomyces cerevisiae , Plc1-derived IP substrate polyphosphate kinase Arg82, converts more complex polyphosphates. study, show in C. enzyme encoded by ARG1 kinase,...
Cryptococcus neoformans strains isolated from patients with AIDS secrete acid phosphatase, but the identity and role of enzyme(s) responsible have not been elucidated. By combining a one-dimensional electrophoresis step mass spectrometry, canonically secreted CNAG_02944 (Aph1), was identified in secretome highly virulent serotype A strain H99. We created an APH1 deletion mutant (Δaph1) showed that Δaph1-infected Galleria mellonella mice survived longer than those infected wild type (WT),...
Invasive fungal diseases (IFD) are a serious threat, but physicians in Asia lack access to many advanced diagnostics mycology. It is likely that they face other impediments the management of IFD. A gap analysis was performed understand challenges Asian faced medical mycology.The Fungal Working Group (AFWG) conducted web-based survey on practices for IFD among clinicians China, India, Indonesia, Philippines, Singapore, Taiwan and Thailand.Among 292 respondents, 51.7% were infectious disease...
Abstract Background In a randomized trial, Lianhuaqingwen (LHQW) capsule was effective for accelerating symptom recovery among patients with coronavirus disease 2019 (COVID-19). However, the lack of blinding and limited sample sizes decreased level clinical evidence. Objectives To evaluate efficacy safety LHQW in adults mild-to-moderate COVID-19. Methods We conducted double-blind controlled trial COVID-19 (17 sites from China, Thailand, Philippine Vietnam). Patients received standard-of-care...