Abstract Introduction The mortality of rhabdomyolysis-induced acute kidney injury (AKI) is still high, as there no effective therapy. It has been shown that bone marrow-derived mesenchymal stem cells (MSCs) can induce M2 macrophages, which mediate MSC protection in other experimental inflammation-related organ injury. This study was designed to investigate the protective effects macrophage activation therapy AKI. Methods MSCs were injected into glycerol-induced rhabdomyolysis mice. Renal...

Significant progress has been made in critical care medicine during the past several decades. However, mortality rate is still high patients with sepsis, especially acute kidney injury (AKI). Mesenchymal stem cells (MSCs) possess an ability to ameliorate renal from ischemia-reperfusion, but it unknown whether they have reduce sepsis-associated AKI.Male C57BL/6 mice underwent cecal ligation and puncture operation induce sepsis then received either normal saline or MSCs (1 × 10 intravenously)...

Adipose-derived stem cells (ASCs) can alleviate acute kidney injury and promote cell regeneration repair. To investigate the role of ASCs in diabetic nephropathy (DN), Sprague-Dawley rats were made by intraperitoneal injection streptozotocin (STZ) after uninephrectomy. After 12 weeks, proteinuria was well established. Five times 5×10(6) human repeatedly injected through a tail vein at 4 weekly intervals. A reduction not observed until 24 weeks. However, urinary protein excretion...

Abstract Malnutrition and acute kidney injury (AKI) are common complications in hospitalised patients, both increase mortality; however, the relationship between them is unknown. This a retrospective propensity score matching study enrolling 46 549 inpatients, aimed to investigate association Nutritional Risk Screening 2002 (NRS-2002) AKI assess ability of NRS-2002 predicting prognosis. In total, 37 190 (80 %) 9359 (20 patients had scores <3 ≥3, respectively. Patients with ≥3 longer...

Sepsis-associated acute kidney injury (SA-AKI) is an independent risk factor for death in patients with sepsis, but treatment it limited. To improve the diagnosis and of SA-AKI, we must first understand its pathogenesis. Recently, interleukin (IL)-17A has been shown to be associated pathogenesis role SA-AKI remains unclear.SA-AKI was induced male C57BL/6 IL-17A(-/-) mice using cecal ligation puncture (CLP) operations 24 h.At 7 days, only seven survived wild-type septic group, nine...

Considerable evidences have indicated that elevated uric acid (UA) was involved in renal tubular injury leading to hyperuricemic nephropathy (HN). Scutellarin is a biologically active flavonoid derived from the Chinese traditional herb Erigeron breviscapus Hand-Mazz, which has been widely used treatment of cardiovascular and cerebrovascular diseases. In present study, we analyzed effect scutellarin on HN, by using C57BL/6 mice human epithelial cell line HK-2 subjected adenine/potassium...

It is well known that the progression of hyperuricemia disease often contributes to renal dysfunction. However, there have been few studies on uric acid nephropathy (UAN), especially its relationship with gut microbiota. UAN usually accompanied by disordered intestinal flora, and damaged barrier, which are closely related tubulointerstitial fibrosis, systemic inflammation. In previous studies, it has confirmed curcumin could alleviate improve function through antioxidant, anti-apoptotic,...

The present study investigated the specific mechanism by which mesenchymal stem cells (MSCs) protect against sepsis-associated acute kidney injury (SA-AKI).Male C57BL/6 mice underwent cecal ligation and puncture surgery to induce sepsis then received either normal IgG or MSCs (1 × 106 cells, intravenously) plus Gal-9 soluble Tim-3 3 h after surgery.After surgery, injected with had a higher survival rate than in treatment group. Treatment decreased serum creatinine blood urea nitrogen levels,...

Aim: To evaluate the protective effects of resveratrol on acute kidney injury (AKI) in septic rats. Methods: A rat model was established by cecal ligation and puncture (CLP). total 108 male Sprague Dawley rats were randomly divided into an observation group, a 6 h intervention group 12 group. Then each subdivided Sham, Sham + Res, CLP Res groups. After surgery, survival morphological changes tissues observed. Serum creatinine urea nitrogen levels, expression GRP78, BiP, IRE1 p65 tissues,...

Objective: The aim of the present study was to investigate protective effect MSCs on CLP-induced SA-AKI and determine mechanisms this effect. Methods: expression Gal-9 Tim-3 assayed by qPCR western blot. IL-10, IL-17, RORγt, FOXP3 were TNFα, INFγ, IL-4, IL-6 ELISA in renal samples after CLP with or without treatment. knocked down vivo using RNA interference, si-Gal-9-MSCs injected mice. differentiation lymphocytes into Th17 cells Tregs evaluated vitro FAC coculture CD4+ T blockade...

Abstract Background We aimed to develop a nomogram based on preprocedural features for early prediction of acute kidney injury (AKI) and assess the prognosis in patients after radical partial nephrectomy. Methods The study included development cohort 1111 who were treated between June 2012 2017 an additional validation 356 July 2018. Stepwise regression logistic analyses used evaluate association predictors AKI. Incorporating all independent predictors, postoperative AKI was developed...

Hepatocellular carcinoma is a common malignant cancer with high incidence. And long non-coding RNAs (lncRNAs) play pivotal roles in the development of different types cancers. In this study, we aimed to investigate role lncRNA maternally expressed gene 3 (MEG3) and progression hepatocellular carcinoma. Expression MEG3 tumor tissues adjacent healthy patients, as well serum both patients controls, was detected by quantitative reverse transcriptase-polymerase chain reaction. The results showed...

Acute kidney injury (AKI) results in high morbidity and mortality among inpatients, while effective treatment intervention are still absent. Therefore, this study aims to explore the effects of Scutellarin (Scu) experimental models vivo vitro.In experiment, we employed a total 30 Wistar rats, which further were modelled by bilateral renal pedicle clip for 45 min, then received 50 mg/kg/day Scu. In vitro, HK-2 cells administered with 20μΜ Scu incubated hypoxia/reoxygenation (H/R) conditions...

ABSTRACT Aim Studies have shown that cysteine‐rich protein 61 (Cyr61) increased in the post‐ischemic human kidney tissue. However, it is still unknown whether Cyr61 can be used as a biomarker ischemia/reperfusion (I/R) injury. Methods Microarray data were collected from GSE58438 and GSE52004. The rat I/R model was established to evaluate messenger RNA expression of Cyr61, localization by immunohistochemical immunofluorescence staining, changes serum creatinine (Scr) at same time. Results...

Hexarelin exhibits significant protection against organ injury in models of ischemia/reperfusion (I/R)-induced (IRI). Nevertheless, the impact on acute kidney (AKI) and its underlying mechanism remains unclear. In this study, we investigated therapeutic potential I/R-induced AKI elucidated molecular mechanisms.We assessed protective effects through both vivo vitro experiments. model, rats were pretreated with at 100 μg/kg/d for 7 days before being sacrificed 24 h post-IRI. Subsequently,...

Objective: To explore the regulation and function of serum response factor (SRF) in epithelial-mesenchymal transition (EMT) renal tubular epithelial cells (TECs) hyperuricemic nephropathy (HN).Results: In NRK-52E treated with UA medulla tissue samples from rats, SRF, fibronectin, α-SMA FSP-1 expression was upregulated, while ZO-1 E-cadherin downregulated.SRF upregulation increased slug expression.Blockade SRF by an SRF-specific siRNA or CCG-1423 reduced induction protected TECs undergoing...

Acute lung injury is a common complication of sepsis in intensive care unit patients. Inflammation among the main mechanisms sepsis. Therefore, suppression inflammation an important mechanism for treatment. Mesenchymal stem cells (MSCs) have been reported to exhibit antimicrobial properties.The present study investigated effects MSCs on sepsis-induced acute injury.Male C57BL/6 mice underwent cecal ligation and puncture (CLP) operation induce then received either normal saline or (1 × 106...

Objective The aim of the study was to establish a predictive postoperative nomogram for acute kidney injury (AKI) after intracranial aneurysm clipping surgery, in order early identify patients with high AKI risk.Methods This is retrospective study, which included who underwent surgery. Multivariate logistic regression employed select confound factors that associated AKI, then incorporated into nomogram. accuracy model assessed by concordance index (C-Index).Results A total 365 surgery were...

Ischemia/reperfusion injury (IRI), a participant in acute kidney (AKI), can occur as series of pathological processes such inflammation. Linarin (LIN) has been widely used for different diseases. To confirm the anti-inflammatory value and relevant mechanism LIN during IRI, vivo vitro models were established. or dissolvent was given, histologic analysis, quantitative (q)RT-PCR, serum creatinine blood urea nitrogen testing to evaluate injury. Microarray protein–protein interaction (PPI)...

Objective To explore the role of cysteine-rich protein 61 (Cyr61) in ischemic renal fibrosis and fibroblasts. Methods (1) The rat model was established after ischemia-reperfusion acute injury (IR-AKI). We detected function by biochemical test, evaluated Masson staining, expression Cyr61 western blotting. (2) Bioinformatics technique adopted to analyze activated (3) Normal kidney fibroblast cells (NRK-49F cells) with over-expression (Cyr61+) low-expression it (Cyr61--) were plasmid...

Abstract The effects of early thiamine use on clinical outcomes in critically ill patients with acute kidney injury (AKI) are unclear. purpose this study was to investigate the associations between administration and AKI. data AKI within 48 h after ICU admission were extracted from Medical Information Mart for Intensive Care III (MIMIC III) database. PSM used match receiving treatment those not treatment. association in-hospital mortality due determined using a logistic regression model. A...