A5048917975- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Bacteriophages and microbial interactions
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Phytoplasmas and Hemiptera pathogens
- Animal Virus Infections Studies
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Soybean genetics and cultivation
- Cocoa and Sweet Potato Agronomy
- Plant Pathogens and Resistance
- Plant and animal studies
- Legume Nitrogen Fixing Symbiosis
- Berry genetics and cultivation research
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
Iwate University
2013-2022
Niigata University
2021
Chinese Academy of Sciences
2015
Wakayama Medical University
2001
Hyogo Prefectural Awaji Medical Center
1998
Institute of Fruit Tree and Tea Science
1997
Minami Kyushu University
1997
Planta
1995
Osaka Prefecture University
1984-1986
Kasetsart University
1986
Members of the family Secoviridae are non-enveloped viruses with mono- or bipartite (RNA-1 and RNA-2) linear positive-sense ssRNA genomes size RNAs combined ranging from 9 to 13.7 kb. They related picornaviruses classified in order Picornavirales. The majority known members infect dicotyledonous plants many important plant pathogens (e.g. grapevine fanleaf virus rice tungro spherical virus). This is a summary current International Committee on Taxonomy Viruses (ICTV) report taxonomy...
Virus-induced gene silencing (VIGS) is an effective technology for the analysis of functions in plants. Though there are many reports on virus vectors VIGS plants, no available Rosaceae fruit trees were reported so far. We present system apple, pear, and Japanese pear using Apple latent spherical (ALSV) vectors.Inoculation ALSV carrying a partial sequence endogenous genes from apple [ribulose-1, 5-bisphosphate carboxylase small subunit (rbcS), alpha chloroplast chaperonin (CPN60a),...
Photoperiodism is a rhythmic change of sensitivity to light, which helps plants adjust flowering time according seasonal changes in daylength and adapt growing conditions at various latitudes. To reveal the molecular basis photoperiodism soybean (Glycine max), facultative short-day plant, we analyzed transcriptional profiles maturity gene E1 family two FLOWERING LOCUS T (FT) orthologs (FT2a FT5a). E1, repressor for FT2a FT5a, its homologs, E1-like-a (E1La) E1Lb, exhibited peaks expression...
Summary Fruit trees have a long juvenile phase. For example, the phase of apple ( M alus × domestica ) generally lasts for 5–12 years and is serious constraint genetic analysis creating new cultivars through cross‐breeding. If modification genes involved in transition from to adult can enable complete its life cycle within 1 year, as seen herbaceous plants, significant enhancement breeding will be realized. Here, we report novel technology that simultaneously promotes expression A rabidopsis...
ABSTRACT Apple latent spherical virus (ALSV) expressing yellow and cyan fluorescent proteins (ALSV-YFP ALSV-CFP) was used to investigate the distribution of identical populations in coinfected plants. In Chenopodium quinoa plants inoculated with a mixture ALSV-YFP ALSV-CFP, fluorescence from YFP CFP always distributed separately both upper uninoculated leaves. Inoculation each ALSV-CFP different leaves C. plant resulted separate population among When were first then reinoculated into same at...
ABCE-class MADS domain transcription factors (MTFs) are key regulators of floral organ development in angiosperms. Aberrant expression these genes can result abnormal traits such as phyllody. Phyllogen is a virulence factor conserved phytoplasmas, plant pathogenic bacteria the class Mollicutes. It triggers phyllody Arabidopsis thaliana by inducing degradation A- and E-class MTFs. However, it still unknown whether phyllogen induce plants other than A. thaliana, although phytoplasma-associated...
Genes in the FLOWERING LOCUS T (FT) family integrate external and internal signals to control various aspects of plant development. In soybean (Glycine max), FT2a FT5a play a major role floral induction, but their roles post-flowering reproductive development remain undetermined. Ectopic overexpression analyses revealed that similarly induced flowering, was markedly more effective than for termination stem growth. The down-regulation Dt1, orthologue Arabidopsis TERMINAL FLOWER1, shoot apices...
A virus with isometric particles (ca. 25 nm) was isolated from an apple tree and named Apple latent spherical (ALSV). Virus purified infected Chenopodium quinoa formed two bands densities of 1·41 1·43 g/cm 3 in CsCl equilibrium density-gradient centrifugation, indicating that the is composed components. The had ssRNA species (RNA1 RNA2) three capsid proteins (Vp25, Vp24 Vp20). complete nucleotide sequences RNA1 RNA2 were determined to be 6815 nt 3384 excluding 3′ poly(A) tail, respectively....
The complete nucleotide sequence of the genome an apple isolate chlorotic leaf spot virus (ACLSV-A) was determined. is 7552 nucleotides excluding poly(A) tail and contains three open reading frames (ORFs 1, 2 3), encoding proteins with M r values 216503 (216∙5K), 50453 (50∙4K) 21394 (21∙4K), respectively. Nucleotide comparisons between ACLSV-A previously sequenced ACLSV from plum (ACLSV-P) showed that identity at level 79∙8%. Amino acid identities ORFs 1 both isolates were 88∙4% 79∙9%, 21.4K...
Apple chlorotic leaf spot virus (ACLSV) is the type species of genus Trichovirus and its single-stranded, plus-sense RNA genome encodes a 216 kDa protein (P216) involved in replication, 50 movement (P50) 21 coat (CP). In this study, it was investigated whether these proteins might have silencing-suppressor activities by Agrobacterium-mediated transient assay green fluorescent protein-expressing Nicotiana benthamiana line 16c. The results indicated that none could suppress local silencing...
The expression of LeATL6, an ortholog Arabidopsis ATL6 that encodes a RING-H2 finger protein, was induced in tomato roots treated with cell wall protein fraction (CWP) elicitor the biocontrol agent Pythium oligandrum. LeATL6 expressed as fusion maltose-binding (MBP) Escherichia coli, and it catalyzed transfer ubiquitin to MBP moiety on incubation ubiquitin, ubiquitin-activating enzyme E1, ubiquitin-conjugating E2; this indicated represents ligase E3. also by treatment jai1-1 mutant cells...
Purple-blue flower of soybean (Glycine max [L.] Merr.) is controlled by the W2 locus. Previous studies revealed that a MYB transcription factor gene GmMYB-G20-1 was located at position similar to and base substitution generated stop codon in domains 2 lines with purple-blue flowers. This study conducted confirm relationship between gene. Cleaved amplified polymorphic sequence analysis detect suggested mutation occurred other having flowers, 037-E-8, Yogetsu 1-blue. Thus, all genotypes...
Plant viral vectors are superior tools for genetic manipulation, allowing rapid induction or suppression of expression a target gene in plants. This is particularly effective technology use breeding fruit trees, which difficult to manipulate using recombinant DNA technologies. We reported previously that if apple seed embryos (cotyledons) infected with an Apple latent spherical virus (ALSV) vector (ALSV-AtFT/MdTFL1) concurrently expressing the Arabidopsis thaliana florigen (AtFT) and...
Generally, double-flowered varieties are more attractive than single-flowered in ornamental plants. Japanese gentian is one of the most popular floricultural plants Japan, and it desirable to breed elite cultivars. In this study, we attempted characterize a doubled-flower mutant gentian. To identify gene that causes phenotype gentian, isolated characterized MADS-box genes.Fourteen genes were isolated, two them C-class (GsAG1 GsAG2). Both GsAG1 GsAG2 categorized into PLE/SHP subgroup, rather...
Apple latent spherical virus (ALSV) vector is a convenient alternative to genetic transformation in horticultural plants, especially species recalcitrant transformation. ALSV, an RNA virus, can infect wide variety of plant including major plants without inducing symptoms. Here, methodologies were developed for infection ALSV vectors strawberry seedlings and plantlets cultured vitro. A seed-propagated F1 hybrid cultivar 'Yotsuboshi' was aseptically grown on half-strength Murashige-Skoog...
Abstract An increasing number of plant viruses and viroids have been reported from all over the world due largely to metavirogenomics approaches with technological innovation. Herein, official changes virus taxonomy, including establishment megataxonomy amendments codes classification nomenclature, recently made by International Committee on Taxonomy Viruses were summarized. The continued efforts virology community Japan index occurring in Japan, which represent 407 viruses, 303 species 104...