- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant pathogens and resistance mechanisms
- Agronomic Practices and Intercropping Systems
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Nematode management and characterization studies
- Plant nutrient uptake and metabolism
- Plant Pathogens and Resistance
- Plant Gene Expression Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- Cassava research and cyanide
- Spectroscopy and Laser Applications
- Plant Disease Resistance and Genetics
- Plant responses to elevated CO2
- Plant Genetic and Mutation Studies
- Plant responses to water stress
- Plant tissue culture and regeneration
- Plant-derived Lignans Synthesis and Bioactivity
- Advanced Measurement and Detection Methods
Guangzhou University
2018-2024
First Automotive Works (China)
2018
Northeast Agricultural University
2015-2018
Soybean (Glycine max) serves as a major source of protein and edible oils worldwide. The genetic genomic bases the adaptation soybean to tropical regions remain largely unclear. Here, we identify novel locus Time Flowering 16 (Tof16), which confers delay flowering improve yield at low latitudes determines that it harbors homolog LATE ELONGATED HYPOCOTYL (LHY). Tof16 previously identified J genetically additively but independently control under short-day conditions. More than 80% accessions...
Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.], caused by sojae Kaufmann Gerdemann, is a destructive disease throughout the planting regions in world. Here, we report insights into function underlying mechanisms novel ethylene response factor (ERF) soybean, namely GmERF5, host responses to P. sojae. GmERF5-overexpressing transgenic exhibited significantly enhanced resistance positively regulated expression PR10, PR1-1, PR10-1 genes. Sequence analysis suggested that GmERF5...
Soybean (Glycine max) is an economically important oil and protein crop. Plant height a key trait that significantly impacts the yield of soybean; however, research on molecular mechanisms associated with soybean plant lacking. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated system 9) recently developed technology for gene editing has been utilized to edit genomes crop plants.Here, we designed four gRNAs mutate LATE ELONGATED HYPOCOTYL (LHY)...
Isoflavone reductase (IFR) is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin plants. IFRs are unique to plant kingdom and considered have crucial roles response various biotic abiotic environmental stresses. Here, we report characterization a novel member soybean isoflavone gene family GmIFR. Overexpression GmIFR transgenic exhibited enhanced resistance Phytophthora sojae. Following stress treatments, was significantly induced by P. sojae, ethephon (ET), abscisic...
Flowering time and plant height are key agronomic traits that directly affect soybean (Glycine max) yield. APETALA1 (AP1) functions as a class A gene in the ABCE model for floral organ development, helping to specify carpel, stamen, petal, sepal identities. There four AP1 homologs soybean, all of which mainly expressed shoot apex. Here, we used clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR-associated protein 9 technology generate homozygous quadruple mutant,...
Photoperiod sensitivity is a key factor in plant adaptation and crop production. In the short-day soybean, to low latitude environments provided by mutations at J locus, which confer extended flowering phase thereby improve yield. The identity of as an ortholog Arabidopsis ELF3, component circadian evening complex (EC), implies that orthologs other EC components may have similar roles. Here we show two soybean homeologs LUX ARRYTHMO interact with form EC. Characterization mutants reveals...
The circadian clock plays essential roles in diverse plant biological processes, such as flowering, phytohormone biosynthesis and abiotic stress responses. manner which genes regulate drought responses model plants has been well established, but comparatively little is known crop species, soybean, a major global crop. This paper reports that the core components GmLHYs, orthologues of CCA1/LHY Arabidopsis, negatively control tolerance soybean. expressions four GmLHYs were all induced by...
Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor. In particular, genetic basis adaptation in remains poorly understood. this study, by combining whole-genome resequencing genome-wide association studies we identified novel locus, Time Flowering 5 (Tof5), which promotes flowering enhances to high latitudes both soybean. By genomic, transgenic analyses showed that Tof5 encodes homolog Arabidopsis thaliana FRUITFULL...
Photoperiod is an important environmental cue. Plants can distinguish the seasons and flower at right time through sensing photoperiod. Soybean a sensitive short-day crop, timing of flowering varies greatly different latitudes, thus affecting yields. cultivars in high latitudes adapt to long day by impairment two phytochrome genes, PHYA3 PHYA2 , legume-specific suppressor, E1 . However, regulating mechanism underlying phyA soybean remains largely unknown. Here, we classified regulation...
Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) one the most extensively studied enzymes related to plant responses biotic abiotic stresses. However, molecular mechanism PAL in response P. largely unclear. Here, we characterize novel member gene family, GmPAL2.1, which significantly induced sojae. Overexpression RNA interference analysis demonstrates that GmPAL2.1 enhances resistance...
Phytophthora root and stem rot caused by the oomycete pathogen sojae is a destructive disease of soybean worldwide. Plant dirigent proteins (DIR) are proposed to have roles in biosynthesis either lignan or lignin-like molecules, important for defense responses, secondary metabolism, resistance. In present work, novel DIR gene expressed sequence tag (EST) identified as up-regulated highly resistant cultivar 'Suinong 10' inoculated with P. sojae. The full length cDNA isolated using rapid...
Phytophthora root and stem rot of soybean caused by the oomycete sojae, is a destructive disease worldwide. Ethylene response factors (ERFs) play important roles in regulating plant biotic abiotic stress tolerance. In this study, new ethylene factor gene, GmERF113, was isolated from highly resistant 'Suinong 10'. Sequence analysis suggested that protein encoded GmERF113 contained conserved AP2/ERF domain 58 amino acid belonged to B-4 subgroup ERF subfamily. Expression significantly induced...
Pathogenesis-related proteins (PR proteins) play crucial roles in the plant defense system. A novel PRP gene was isolated from highly resistant soybean infected with Phytophthora sojae (P. sojae) and named GmPRP (GenBank accession number: KM506762). The amino acid sequences of showed identities 74%, 73%, 72% 69% Vitis vinifera, Populus trichocarpa, Citrus sinensis Theobroma cacao, respectively. Quantitative real-time reverse transcription PCR (qRT-PCR) data that expression highest roots,...
Phytophthora sojae Kaufmann and Gerdemann causes root rot, a destructive soybean disease worldwide. A basic helix-loop-helix (bHLH) transcription factor is thought to be involved in the response P. infection soybean, as revealed by RNA sequencing (RNA-seq). However, molecular mechanism underlying this currently unclear. Here, we explored function mechanisms of bHLH designated GmPIB1 (P. sojae-inducible factor), during host responses sojae. was significantly induced resistant cultivar...
Salt stress and flowering time are major factors limiting geographic adaptation yield productivity in soybean (Glycine max). Although improving crop salt tolerance latitude essential for efficient agricultural production, whether how these two traits integrated remains largely unknown. Here, we used a genome-wide association study to identify salt-tolerance locus controlled by E2, an ortholog of Arabidopsis thaliana GIGANTEA (GI). Loss E2 function not only shortened maturity, but also...
In many plants, flowering time is influenced by daylength as an adaptive response. soybean (Glycine max) cultivars, however, photoperiodic reduces crop yield and quality in high-latitude regions. Understanding the genetic basis of wild soja) adaptation to high latitudes could aid breeding improved cultivars. Here, we identify Tof4 (Time 4) locus, which encodes E1-like protein, E1La, that represses enhances soybean. Moreover, found physically associates with promoters two important FLOWERING...
Genes in the FLOWERING LOCUS T (FT) family integrate external and internal signals to control various aspects of plant development. In soybean (Glycine max), FT2a FT5a play a major role floral induction, but their roles post-flowering reproductive development remain undetermined. Ectopic overexpression analyses revealed that similarly induced flowering, was markedly more effective than for termination stem growth. The down-regulation Dt1, orthologue Arabidopsis TERMINAL FLOWER1, shoot apices...
Soybean [Glycine max (L.) Merr.] is an important crop for oil and protein resources worldwide, its farming impacted by increasing soil salinity levels. In Arabidopsis the gene EARLY FLOWERING 3 (ELF3), increased salt tolerance suppressing stress response pathways. J ortholog of AtELF3 in soybean, loss-of-function J-alleles greatly prolong soybean maturity enhance grain yield. The exact role abiotic however, remains unclear. this study, we showed that expression was induced NaCl treatment...
Soybean (Glycine max) is an important legume crop that was domesticated in temperate regions. varieties from these regions generally mature early and exhibit extremely low yield when grown under inductive short-day (SD) conditions at latitudes. The long-juvenile (LJ) trait, which characterized by delayed flowering maturity, improved SD conditions, allowed the cultivation of soybean to expand lower Two major loci control LJ trait: J E6. In current study, positional cloning, sequence analysis,...
Salt stress is a major factor limiting the growth and yield of soybean (Glycine max). Wild soybeans soja) contain high allelic diversity beneficial alleles that can be re-introduced into domesticated to improve adaption environment. However, very few have been identified from wild soybean. Here, we demonstrate more salt tolerant than cultivated examine dehydration responsive element-binding (DREB) family transcription genes look for advantageous might drought tolerance in Our genome-wide...
Summary Flowering time is one of important agronomic traits determining the crop yield and affected by high temperature. When facing ambient temperature, plants often initiate early flowering as an adaptive strategy to escape stress ensure successful reproduction. However, here we find opposing ways in short‐day soybean respond different levels temperatures, which accelerates when temperature changes from 25 30 °C, but delays reaches 35 °C under short day. phyA‐E1, possibly photoperiodic...