Hepatocellular carcinoma (HCC) is the fifth most common solid cancer and third cause of cancer-related mortality. HCC develops via a multistep process associated with genetic aberrations that facilitate invasion migration promote metastasis. A growing body evidence indicates stem cells (CSCs) are responsible for tumorigenesis, cell Despite extremely small proportion represented by this subpopulation cells, CSCs play key role in metastasis poor prognosis. ELK3 (Net/SAP-2/Erp) transcription...

Abstract Liver cancer stem cells (LCSCs) have attracted attention because they cause therapeutic resistance in hepatocellular carcinoma (HCC). Understanding the metabolism of LCSCs can be a key to developing strategy, but metabolic characteristics not yet been studied. Here, we systematically analyzed and compared global phenotype between non-LCSCs using transcriptome metabolome data. We also reconstructed genome-scale models (GEMs) for LCSC non-LCSC comparatively examine differences their...

Nonalcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome. Studies have demonstrated that anthocyanin-rich foods may improve hyperlipidemia and ameliorate steatosis. Here, effects Aronia melanocarpa (AM), known to be rich anthocyanins, on lipid metabolism adipogenic genes were determined. AM was treated C57BL/6N mice fed with high fat diet (HFD) or FL83B cells free acid (FFA). Changes in levels lipids, enzymes hormones observed, expressions involved detected...

Wnt/β-catenin (CTNNB1) signaling is crucial for the proliferation and maintenance of intestinal stem cells (ISC), but excessive activation leads to ISC expansion eventually colorectal cancer. Thus, negative regulators are required maintain optimal levels signaling. Aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMP) function in protein synthesis, have also been implicated cascades affecting angiogenesis, immunity, apoptosis. In this study, we investigated relationship...

Abstract Virus-like particles (VLPs) possess great potential for organ-specific transport of therapeutic agents due to their central cavity surrounded by viral capsid proteins and similar tropism original viruses. The N-terminal truncated second open reading frame (Nt-ORF2) the hepatotropic hepatitis E virus (HEV) forms VLPs via self-assembly. In present study, we investigated whether HEV-LPs could deliver foreign genes specifically liver. were obtained from Nt-ORF2 expression in Huh7 cells...

Arnica montana (AM), which belongs to the daisy family Asteraceae, has a longstanding traditional use in Europe and North America for pain inflammation treatment. This study investigates inhibitory effects of ‘Arnica extract hydrogel patch (AHP)’ on Carrageenan-induced paw edema hot plate-induced models. AHP exhibited transdermal permeability without occurrence issues like crystal precipitation. employed two animal model assessments using AHP, comparison with Arnicare Gel (AG), evaluate...

Background/Aims: The role of Elk-3 in the epithelial-mesenchymal transition (EMT) during liver fibrogenesis remains unclear.Here, we determined expression vitro and vivo models human fibrotic tissues.We also investigated molecular relationships among Elk-3, early growth response-1 (Egr-1), mitogen activated protein kinases (MAPK) pathway EMT hepatocytes.Methods: We established an model which normal mouse hepatocyte cell lines were treated with transforming factor (TGF)-β1 a CCl 4 -induced...

One of the most important factors in production cartilage is transforming growth factor β1 (TGF-β1). To obtain sustained release TGF-β1, a cell-mediated gene therapy technique was introduced. We infected chondrocytes with retroviral vector carrying TGF-β1 gene. The single clone derivative showed secretion. It also constitutive type II collagen expression. Whereas protein itself unable to induce formation vivo, human engineered express encoding vivo when cells were injected into nude mice...

N-myc downstream regulated gene 2 (NDRG2) is a member of the NDRG family. Our previous report indicated possible role for NDRG2 in regulating cytokine, interleukin-10 (IL-10), which an important immunosuppressive cytokine. Several pathways, including p38-MAPK, NF-κB, and JAK/STAT, are used IL-10 production, JAK/STAT pathway can be inhibited negative feedback loop by inducible protein, SOCS3. In present study, we investigated effect expression on signaling that modulated via SOCS3 STAT3.We...

The epithelial-mesenchymal transition (EMT) is involved in many different types of cellular behavior, including liver fibrosis. In this report, we studied a novel function RAR-related orphan receptor gamma (ROR-γ) hepatocyte EMT during To induce vitro, primary hepatocytes and FL83B cells were treated with TGF-β1. Expression ROR-γ was analyzed by Western blot the fibrotic mouse livers human cirrhosis. verify role EMT, silenced using lentiviral short hairpin RNA (shRNA) vector. therapeutic...

Hepatitis C virus (HCV) replicates in the peripheral blood mononuclear cells (PBMCs), leading to production of type I interferons (IFNs). It is well known that gene expression profile PBMC similar liver. The present study explored dynamic PBMCs collected from HCV-infected patients undergoing direct-acting antiviral (DAA) therapy.A prospective cohort comprising 27 under DAA therapy was formed. Expression level IFN-β and its downstream interferon-stimulated genes (ISGs) measured before after...

In this study, we aimed to detect and characterize ex vivo virus-specific CD8+ T cells in patients with immune-tolerant hepatitis B virus (HBV) infection. We investigated a Korean chronic cohort composed of 15 the phase, 17 immune-active 13 under antiviral treatment. performed enzyme-linked immunospot (ELISpot) assays intracellular cytokine staining after vitro culture. also multimer examined expression programmed death-1 (PD-1) CD127 pentamer-positive cells. Ex ELISpot revealed that...

Proactively predicting antidepressant treatment response before medication failures is crucial, as it reduces unsuccessful attempts and facilitates the development of personalized therapeutic strategies, ultimately enhancing efficacy. The current decision-making process, which heavily depends on subjective indicators, underscores need for an objective, indicator-based approach. This study developed a method detecting depression through deep learning-based spectroscopic analysis extracellular...

In this study, we investigated the role of microRNA-99a (miR-99a) in hepatitis C virus (HCV) replication and lipogenesis hepatocytes. Cell-culture-derived HCV (HCVcc) infection caused down-regulation miR-99a Huh-7 cells, relative levels were significantly lower sera HCV-infected patients than those healthy controls. Transfection miR-99a-5p mimics resulted a decrease intracellular secreted RNA levels. It also decreased mammalian target rapamycin (mTOR) protein level phosphorylation its...

8-Hydroxy-2-methoxy-1,4-naphthoquinone (HMNQ), a natural compound isolated from the bark of Juglans sinensis Dode, displays cytotoxic activity against various human cancer cells. However, molecular mechanism anticancer effect is unclear. In this study, we examined HMNQ at level in Cells were treated with dose- or time-dependent manner. treatment inhibited cell viability, colony formation and migration, indicating that induced death. HMNQ-treated cells resulted apoptotic death through PARP-1...

Despite the discovery of hepatitis C virus (HCV) entry factor, mechanism by which it is regulated miRNAs remains unclear. Adipose tissue-derived human mesenchymal stem cells (AT-hMSCs) have been widely used for differentiated hepatocyte-like (DHCs). Here, we established an in vitro HCV infection model using DHCs from AT-hMSCs and identified that modulate infectivity.AT-hMSCs were into conditional media, evaluated hepatocyte characteristics RT-PCR, immunocytochemistry, periodic acid-Schiff...

Metallic fuel has been considered for sodium-cooled fast reactors because it can maximize the uranium resources. It generates rare earth elements as fission products, where is reported by aggravating fuel–cladding chemical interaction at operating temperature. Rare form a multicomponent alloy (Ce–Nd–Pr–La–Sm–etc.) during reactor operation, shows higher reaction thickness than single element. Experiments have carried out simplifying alloys mono or binary systems complex difficulty in...

Unstructured text in medical records, such as Electronic Health Records, contain an enormous amount of valuable information for research; however, it is difficult to extract and structure important because frequent typographical errors. Therefore, improving the quality data with errors analysis essential task. To date, few prior studies have been conducted addressing this. Here, we propose a new methodology extracting from unstructured texts by overcoming problem surgical pathology records...

Inflammatory bowel disease is defined by inflammation and immune dysregulation. This study investigated the effects of Gα13 liver-specific knockout (LKO) on proximal distal colons dextran sodium sulfate (DSS)-induced mice in conjunction with a high-fat diet (HFD). HFD improved body weight gain activity index scores. Gα13LKO exerted no improvement. In colon, augmented DSS effect Il6, which was not observed mice. plus oppositely fortified an increase Tnfa Cxcl10 mRNA but WT. Il6 levels...

Apios americana Medik tubers are medicinal foods with anticancer and anti-inflammatory activities. However, mechanisms of immunostimulatory action the tuber extract (ATE) on macrophages have not been elucidated. In present study, we investigated whether ATE could modulate immune responses, such as production nitric oxide (NO), proinflammatory cytokines, transcription factors, in RAW264.7 macrophage cells. significantly increased NO cytokines interleukin-1β (IL-1β), IL-6, tumor necrosis...

Background In the analysis of electronic health records, proper labeling outcomes is mandatory. To obtain information from radiologic reports, several studies were conducted to classify reports using deep learning. However, classification pneumonia in bilingual has not been previously. Objective The aim this research was into or no a learning method. Methods A data set radiology for chest computed tomography and x-rays surgical patients January 2008 2018 Asan Medical Center Korea...

Abstract Background Unstructured texts in the medical fields such as Electronic Health Records contain an enormous amount of valuable information for research, but it is difficult to extract and structure important because frequent typos. For text analysis, improving quality data with errors essential task, just a few prior studies have been conducted so far. Therefore, we propose new methodology extracting from unstructured by overcoming typographical problem surgical pathology records...