Background— Cardiac consequences of obesity include inflammation, hypertrophy, and compromised energy metabolism. Glucagon-like peptide-1 is an incretin hormone capable cytoprotective actions that reduces inflammation endoplasmic reticulum stress in other tissues. Here we examine the cardiac effects glucagon-like analog liraglutide a model obesity, independent changes body weight. Methods Results— C57Bl6 mice were placed on 45% high-fat diet (HFD) or regular chow diet. Mice HFD developed...

ABSTRACT Sirtuin 1 (Sirt1), a protein deacetylase, is novel target for bone metabolism. To investigate whether overexpression of Sirt1 in mandibular mesenchymal stem cells (M-MSCs) increased alveolar mass vivo, we generated transgenic mice (Sirt1TG), with gene expression driven by the Prx1 gene, which represents lineage. Our results demonstrated that M-MSCs volume 1-month-old, 9-month-old, and 18-month-old Sirt1TG compared age-matched wild-type (WT) mice, ovariectomized WT stimulating M-MSC...

Vitamin D is critical for bone homeostasis and immunomodulation. We therefore assessed whether 1,25-dihydroxyvitamin (1,25(OH)2 D) deficiency in mice with targeted deletion of the gene encoding 25-hydroxyvitamin D-1α-hydroxylase (1α(OH)ase [1αOH)ase-/- mice]) results alveolar loss periodontal inflammation vivo.Ten-week-old 12-month-old 1α(OH)ase-/- wild-type littermates were fed a normal diet or rescue diet, phenotype periodontium was then analyzed using microcomputed tomography, histology,...

Osteoporosis is a common disorder characterized by compromised bone strength that predisposes patients to increased fracture risk. Parathyroid hormone related protein (PTHrP) one of the candidates for clinical osteoporosis treatment. In this study, GST Gene Fusion System was used express recombinant human PTHrP (hPTHrP) 1-34 and 1-84. To determine whether hPTHrP1-34 1-84 can enhance renal calcium reabsorption promote formation, we examined effects on osteogenic lineage commitment in primary...

Abstract Sperm preparation in IVF cycles using density gradient centrifugation (DGC) combination with swim-up (SU) has been widely adopted reproductive centres worldwide. It is a fact that the sperm recovery rate following one DGC from poor semen samples (showing liquefaction defects/containing too many unresolvable clots or rare sperm) relatively low. Our results showed double (DDGC) effective at increasing samples. However, DDGC may increase mechanical stress of sperm, thereby potentially...

To explore the mechanism of bone anabolic action p27 deficiency, we first confirmed that osteoblast formation and osteogenesis were significantly increased in deficient mice compared with their wild-type littermates. Microarray analysis differential gene expression profiles, followed by real-time RT-PCR Western blots revealed deletion upregulated Sonic hedgehog (Shh), Gli1 2 target Bmi1 tissue, down regulated negative regulators Shh pathway Sufu, Patched 1 Gli3 tissue. The antagonist...

To determine whether the action of PTHrP nuclear localization sequence and C terminus is mediated through p27 in modulating dental mandibular development, compound mutant mice, which are homozygous for both deletion PTHrP1-84 knock-in mutation (p27(-/-)Pthrp(KI/KI)), were generated. Their teeth phenotypes compared with those p27(-/-), Pthrp(KI/KI), wild-type mice. At 2 weeks age, mineral density, alveolar bone volume, osteoblast numbers, dentin sialoprotein-immunopositive areas first molar...

OBJECTIVE We aimed to identify the features of micro RNA (mi ) at different fibrotic stages in patients with hepatitis B virus ( HBV )‐related liver fibrosis. METHODS Liver tissues were collected from 40 chronic CHB S 0–4. Microarrays mi s and genomic informatics analysis performed. RESULTS In total, 105 differentially expressed 1–4 groups) compared no 0 group; P < 0.05). Combined three classifications, 17 differential found be closely related (over twofold change Five had a signature...

There are anatomical and functional differences between human dental pulp (DP) periodontal ligament (PDL). However, the molecular biological function of these tissues poorly understood. In present study, we employed a cDNA microarray array to screen for differentially expressed genes (DEGs) DP PDL tissues, used online software WebGestalt perform analysis DEGs. addition, STRING database KEGG pathway were applied interaction network samples obtained from permanent premolars (n=16) extracted...

Osteoblastic lineage cells (OBCs) are bone-building and essential component of hematopoietic niche, but mechanisms whereby hematopoiesis-supportive activities OBCs could be regulated simultaneously remain largely unknown.Here we found that B cell-specific Moloney murine leukemia virus integration site 1 (Bmi1) was involved in such a co-regulatory mechanism.In this study, first that, accompanied with marked decline osteogenic activity, the niche Bmi1 knockout (KO) mice severely impaired...

Quantum Key Distribution (QKD) is a recent technology for secure distribution of symmetric keys, which currently being deployed to increase communications security against quantum attacks. However, the key rate achievable over weak signal limited by link performance (e.g., loss and noise) propagation distance, especially in multi-node QKD networks, making it necessary design scheme efficiently timely distribute keys various nodes. In this work, we formulate, using Mixed Integer Linear...

In renal cell carcinoma, chromophobe carcinoma (ChRCC) is a distinct subtype, whose clinical manifestations often lack specificity, and the molecular mechanisms of ChRCC tumorigenesis remain generally vague. The target this study was to discover novel biomarkers involved in by integrated bioinformatics analyses. We found 2608 differentially expressed genes (DEGs), which 1518 were upregulated 1090 downregulated. Gene ontology (GO) analysis DEGs uncovered significant functional enrichment...

We evaluated the effect of microRNA (miR)-9 inhibition on fracture healing in a rat model femoral fracture. The rats were divided into sham, negative control and miR-9 inhibitor groups. group received 30 pmol/mL intrathecally for 8 consecutive weeks following surgery-induced was estimated by determining bone mineral density (BMD) performing X-ray analysis fractured bone. serum levels markers formation enzyme-linked immunosorbent assay reverse transcription polymerase chain reaction, western...

Purpose: To examine the fine anatomic structures between levator aponeurosis and Müller muscle in front of tarsus. Materials Methods: Postmortem specimens 6 Chinese males (5 elderly men, aging from 68 to 86 years; 1 child, 10 years old) were used. A 3-μm thickness sagittal section central part upper eyelid was prepared, samples examined microscopically by using hematoxylin–eosin, Masson trichrome, anti-smooth actin antibodies staining. Results: There are 2 new findings this study, one is...