A5013421877- Mesenchymal stem cell research
- Telomeres, Telomerase, and Senescence
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Renal and related cancers
- Pluripotent Stem Cells Research
- Acute Kidney Injury Research
- Tissue Engineering and Regenerative Medicine
- IL-33, ST2, and ILC Pathways
- Pancreatic and Hepatic Oncology Research
- Genetics and Neurodevelopmental Disorders
- Histone Deacetylase Inhibitors Research
- Metabolomics and Mass Spectrometry Studies
- Cancer-related gene regulation
- Electrospun Nanofibers in Biomedical Applications
- Nuclear Receptors and Signaling
- Vitamin C and Antioxidants Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gut microbiota and health
- Cardiovascular Disease and Adiposity
- RNA modifications and cancer
Nanjing Medical University
2016-2025
State Key Laboratory of Reproductive Medicine
2017-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
McGill University Health Centre
2019
Occupational Cancer Research Centre
2019
University of Chicago
1989
Abstract To study whether TGF-β1/IL-11/MEK/ERK (TIME) signaling mediates senescence-associated pulmonary fibrosis (SAPF) in Bmi-1- deficient ( Bmi-1 −/− ) mice and determines the major downstream mediator of crosstalk between p16 INK4a reactive oxygen species that regulates SAPF, phenotypes were compared among 7-week-old double-knockout, N -acetylcysteine (NAC)-treated , wild-type mice. Pulmonary fibroblasts alveolar type II epithelial (AT2) cells used for experiments. Human tissues tested Ι...
In parathyroid hormone-related protein 1-84 [PTHrP(1-84)] knockin mice, expression of the polycomb Bmi-1 is reduced and potentially can mediate phenotypic alterations observed. We have therefore now examined skeletal phenotype Bmi-1(-/-) mice in vivo also assessed function bone marrow mesenchymal stem cells (BM-MSCs) from ex culture. Neonatal exhibited growth retardation, with chondrocyte proliferation increased apoptosis. Osteoblast numbers; gene alkaline phosphatase, type I collagen,...
Epigenetic integrity is critical for many eukaryotic cellular processes. An important question how different epigenetic regulators control development and influence disease. Lysine acetyltransferase 8 (KAT8) acetylation of histone H4 at lysine 16 (H4K16), an evolutionarily conserved mark. It unclear what roles KAT8 plays in cerebral human Here, we report that cerebrum-specific knockout mice displayed hypoplasia the neocortex hippocampus, along with improper neural stem progenitor cell (NSPC)...
Abstract Determining the mechanism of senescence‐associated pulmonary fibrosis is crucial for designing more effective treatments chronic lung diseases. This study aimed to determine following: whether Sirt1 and serum vitamin D decreased with physiological aging, promoting by activating TGF‐β1/IL‐11/MEK/ERK signaling, overexpression prevented signaling‐mediated in D‐deficient ( Cyp27b1 −/− ) mice, downregulated IL‐11 expression transcribed TGF‐β1/Smad2 signaling through deacetylating histone...
To determine whether Bmi-1 deficiency could lead to renal tubulointerstitial injury by mitochondrial dysfunction and increased oxidative stress in the kidney, 3-week-old Bmi-1(-/-) mice were treated with antioxidant N-acetylcysteine (NAC, 1 mg mL(-1) ) their drinking water, or pyrro-quinoline quinone (PQQ, 4 kg(-1) diet) diet for 2 weeks, phenotypes compared vehicle-treated Bmi1(-/-) wild-type mice. was knocked down human proximal tubular epithelial (HK2) cells which mm NAC 72 96 h, control...
To determine whether transplanted amniotic membrane mesenchymal stem cells (AMSCs) ameliorated the premature senescent phenotype of Bmi-1-deficient mice, postnatal 2-day-old Bmi-1(-/-) mice were injected intraperitoneally with second-passage AMSCs from membranes β-galactosidase (β-gal) transgenic or wild-type (WT) labeled DiI. Three reinjections given, once every seven days. Phenotypes 5-week-old β-gal(+) AMSC-transplanted 6-week-old DiI(+) compared vehicle-transplanted and WT mice....
Impaired apoptosis of fibroblast-like synoviocytes (FLSs) causes synovial hyperplasia, facilitating destruction cartilage and bone in rheumatoid arthritis (RA). Tumor necrosis factor (TNF)-α, a dominant inflammatory mediator RA pathogenesis, promotes progression symptoms. Prevalence 1, 25-dihydroxy-vitamin D3 (hereafter termed VD) deficiency is 30-63% patients with RA. Whether VD leads to or enhances TNF-α-mediated FLSs ameliorate unclear. To determine this, 10-week-old CYP27B1-deficient...
Chronic changes caused by a high-fat diet (HFD) may be associated with weakened lung function in obese patients. However, few studies have focused on the role of senescent cells HFD-induced pulmonary fibrosis. This study aimed to determine whether (i) obesity causes accumulation aging lungs, (ii) p16 epithelial or fibroblasts exacerbates long-term senescence-associated fibrosis (SAPF) and (iii) deletion clearance ameliorates SAPF through inactivation inflammasome metabolic...
The excess production of reactive oxygen species (ROS) will delay tooth extraction socket (TES) healing. In this study, we developed an injectable thermosensitive hydrogel (NBP@BP@CS) used to treat TES formulation incorporated black phosphorus (BP) nanoflakes, recognized for their accelerated alveolar bone regeneration and ROS-scavenging properties, dl-3-n-butylphthalide (NBP), a vasodilator aimed at enhancing angiogenesis. vivo investigations strongly demonstrated that NBP@BP@CS improved...
To determine whether p16 INK4a deletion ameliorated renal tubulointerstitial injury by inhibiting a senescence-associated secretory phenotype (SASP) in Bmi-1-deficient (Bmi-1 -/-) mice, phenotypes were compared among 5-week-old Bmi-1 and double-knockout, -/- wild-type mice. Fifth-passage interstitial fibroblasts (RIFs) from the three groups analyzed for senescence proliferation. The effect of deficiency on epithelial-to-mesenchymal transition (EMT) was examined Bmi-1-knockdown human proximal...
Abstract Aims Senescence‐associated pathological cardiac hypertrophy (SA‐PCH) is associated with upregulation of foetal genes, fibrosis, senescence‐associated secretory phenotype (SASP), dysfunction and increased morbidity mortality. Therefore, we conducted experiments to investigate whether GATA4 accumulation induces SA‐PCH, Bmi‐1‐RING1B promotes ubiquitination its selective autophagic degradation prevent SA‐PCH. Methods results Bmi‐1 ‐deficient ( −/− ), transgenic overexpressing Tg )...
Periodontitis, a prevalent inflammatory condition in the oral cavity, is closely associated with oxidative stress-induced tissue damage mediated by excessive reactive oxygen species (ROS) production. The jaw vascular unit (JVU), encompassing both and lymphatic vessels, plays crucial role maintaining fluid homeostasis contributes to pathological process diseases of jaw. This study presents novel approach for treating periodontitis through development an injectable thermosensitive gel...
Abstract With the increase in aging population, senile osteoporosis (SOP) has become a major global public health concern. Here, it is found that Prx1 and Bmi‐1 co‐localized trabecular bone, bone marrow cavity, endosteum, periosteum. ‐driven knockout bone‐marrow mesenchymal stem cells (BMSCs) reduced mass increased adiposity by inhibiting osteoblastic formation, promoting osteoclastic resorption, downregulating proliferation osteogenic differentiation of BMSCs, upregulating adipogenic BMSCs....
Physiologically aged lungs are prone to senescence-associated pulmonary diseases (SAPD). This study aimed determine the mechanism and subtype of T cells affecting alveolar type II epithelial (AT2) cells, which promote pathogenesis fibrosis (SAPF). Cell proportions, relationship between SAPD aging- secretory phenotype (SASP) young mice were analyzed using lung single-cell transcriptomics. was monitored by markers AT2 found be induced cells. Furthermore, IFNγ signaling pathways activated cell...
New immunosenescence targets for preventing senescence-associated pathological cardiac hypertrophy (SA-PCH) need to be explored. In the present study, with physiologically aged human and mouse samples, IL-17A level increased physiological aging, heart failure (HF), SA-PCH was negatively correlated thymic Bmi-1 expression. f/f LckCre + mice littermates were generated determine whether delayed T cell aging by maintaining development prevent SA-PCH. As a result, promoted upregulating Notch...
To develop an effective therapeutic strategy for cardiac regeneration using bone marrow mesenchymal stem cells (BM-MSCs), the primary mouse BM-MSCs (1st BM-MSCs) and 5th passage from β-galactosidase transgenic mice were respectively intramyocardially transplanted into acute myocardial infarction (AMI) model of wild type mice. At 6th week, animals/tissues 1st group, control group examined. Our results revealed that, compared to BM-MSCs, had better effects in MI model. The maintained greater...
ABSTRACT We analyzed the skeletal phenotypes of heterozygous null Cyp27b1 (Cyp27b1+/−) mice and their wild-type (WT) littermates to determine whether haploinsufficiency accelerated bone loss, examine potential mechanisms such loss. found that serum 1,25-dihydroxyvitamin D [1,25(OH)2D] levels were significantly decreased in aging Cyp27b1+/− mice, which displayed an osteoporotic phenotype. This was accompanied by a reduction expression B lymphoma Moloney murine leukemia virus (Mo-MLV)...
We report that cerebrum-specific inactivation of the histone deacetylase 3 (HDAC3) gene causes striking developmental defects in neocortex, hippocampus, and corpus callosum; post-weaning lethality; abnormal behaviors, including hyperactivity anxiety. The are due to rapid loss embryonic neural stem progenitor cells (NSPCs). Premature neurogenesis neuronal migration mutant brain alter NSPC homeostasis. Mutant cerebral cortices also display augmented DNA damage responses, apoptosis,...