A5079911042- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Plant-Microbe Interactions and Immunity
- Hormonal Regulation and Hypertension
- Bone health and treatments
- Enzyme Structure and Function
- Aldose Reductase and Taurine
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Molecular Research
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Steroid Chemistry and Biochemistry
- Advanced Data Storage Technologies
- Protein Structure and Dynamics
- Advanced Nanomaterials in Catalysis
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- PARP inhibition in cancer therapy
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Cancer-related gene regulation
- Plant Parasitism and Resistance
Southern Medical University
2025
Nanfang Hospital
2025
Zhejiang Cancer Hospital
2024-2025
Chinese Academy of Sciences
1999-2025
Theranostics (New Zealand)
2025
Shanghai Ocean University
2024-2025
Hong Kong Baptist University
2017-2024
China Medical University
2024
Shaoyang University
2022-2024
Ruian People's Hospital
2024
Epigenetic regulators represent a promising new class of therapeutic targets for cancer. Enhancer zeste homolog 2 (EZH2), subunit Polycomb repressive complex (PRC2), silences gene expression via its histone methyltransferase activity. We found that the oncogenic function EZH2 in cells castration-resistant prostate cancer is independent role as transcriptional repressor. Instead, it involves ability to act coactivator critical transcription factors including androgen receptor. This functional...
Osteoporosis and low bone mass are currently estimated to be a major public health risk affecting >50% of the female population over age 50. Because their bone-selective pharmacokinetics, nitrogen-containing bisphosphonates (N-BPs), used as clinical inhibitors bone-resorption diseases, target osteoclast farnesyl pyrophosphate synthase (FPPS) inhibit protein prenylation. FPPS, key branchpoint mevalonate pathway, catalyzes successive condensation isopentenyl with dimethylallyl geranyl...
The development of safe and efficacious gene vectors has limited greatly the potential for therapeutic treatments based on messenger RNA (mRNA). Lipid nanoparticles (LNPs) formed by an ionizable cationic lipid (here DLin-MC3-DMA), helper lipids (distearoylphosphatidylcholine, DSPC, cholesterol), a poly(ethylene glycol) (PEG) have been identified as very promising delivery short interfering (siRNA) in different clinical phases; however, high-molecular weight proven much more demanding. Herein...
Pancreatic cancer is a highly lethal malignancy associated with tissues of the pancreas. Early diagnosis and effective treatment are crucial to improving survival rate patients pancreatic cancer. In previous study, we employed cell-SELEX strategy obtain an ssDNA aptamer termed XQ-2d high binding affinity for Here, first identify CD71 as XQ-2d-binding target. We found that knockdown abolished membrane-bound CD71, rather than total levels. Competitive analysis revealed shares same site on...
In this work, we have developed a truncated DNA aptamer, termed XQ-2d, with high affinity and specificity for pancreatic ductal adenocarcinoma (PDAC).Aptamer XQ-2d selectively binds to PL45 cells dissociation constant in the nanomolar range, as determined by its recognition of tumor mice.Moreover, shows better ratio 40 tissue sections clinical PDAC samples (82.5%) compared initial cell-SELEX selection library (5%).Therefore, can be considered promising candidate tool diagnosis treatment.
Mammals utilize a single phosphopantetheinyl transferase for the posttranslational modification of at least three different apoproteins: carrier protein components cytosolic and mitochondrial fatty acid synthases aminoadipate semialdehyde reductase involved in lysine degradation. We determined crystal structure human transferase, eukaryotic characterized, complexed with CoA Mg(2+), ternary complex ACP. The involvement key residues ligand binding catalysis was confirmed by mutagenesis kinetic...
Background Automated scanning devices and image analysis software provide a means to overcome the limitations of manual semiquantitative scoring immunohistochemistry. Common drawbacks automated imaging systems include an inability classify tissue type segregate cytoplasmic nuclear staining. Methods Immunohistochemistry for membranous marker α-catenin, stathmin Ki-67 was performed on microarrays (TMA) archival formalin-fixed paraffin-embedded comprising 471 (α-catenin stathmin) 511 (Ki-67)...
// Minlan Duan 1, * , Yuqian Long Cai Yang Xiaoqiu Wu 1 Sun Jianglin Li Xiaoxiao Hu Wei Lin Dongmei Han Yifan Zhao Jing Liu 2 Mao Ye Weihong Tan 3, 4 Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing Chemometrics, College of Biology, Chemistry Chemical Engineering, Collaborative Innovation Center Engineering Theranostics, Hunan University, Changsha, 410082, China School Life Sciences, Medical Genetics, Central South 410078, 3 Department Chemistry,...
The nucleotide-binding domain leucine-rich repeat (NLR) immune receptors play important roles in innate plant immunity. activation of NLRs is specifically induced by their cognate effectors released from pathogens. Autoactive are expected to confer broad-spectrum resistance because they do not need activate responses. In this study, we demonstrated that the NLR genes RPS2 and RPM1(D505V) Arabidopsis were autoactive Oryza sativa conferred fungal pathogen Magnaporthe oryzae, bacterial...
During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross cell membrane and is susceptible degradation. Here we use graphene quantum dots (GQDs) functionalized polyethyleneimine (PEI) as a novel system. Our results show that these modified GQDs can be used deliver intact functional Huh-7 hepatocarcinoma cells at low doses and, are not toxic, although cellular...
17beta-hydroxysteroid dehydrogenases (17beta-HSDs) catalyse the conversion of 17beta-OH (-hydroxy)/17-oxo groups steroids, and are essential in mammalian hormone physiology. At present, eleven 17beta-HSD isoforms have been defined mammals, with different tissue-expression substrate-conversion patterns. We analysed type 10 (17beta-HSD10) from humans Drosophila, latter known to be development. In addition hydroxyacyl-CoA dehydrogenase, 3alpha-OH activities sex we here demonstrate novel...
The interaction of β-catenin with T-cell factor (Tcf) 4 plays a central role in the Wnt signaling pathway and has been discussed as possible site intervention for development anti-cancer drugs. In this study, we performed Ala-scanning mutagenesis all Tcf4 residues Tcf-β-catenin interface studied binding energetics these mutants using isothermal titration calorimetry. Binding was found to be highly cooperative. Single mutations most resulted significant reduction enthalpies but similar...
Abstract Prostate cancer is an ideal target for chemoprevention. To date, chemoprevention clinical trials with 5α-reductase inhibitors have yielded encouraging yet ultimately confounding results. Using a preclinical mouse model of high-grade prostatic intraepithelial neoplasia (HG-PIN) induced by PTEN loss, we observed unprecedented deteriorating effects androgen deprivation, in which surgical castration or MDV3100 treatment accelerated disease progression the otherwise stable HG-PIN to...
Patients after head and neck tumor reconstruction surgery frequently require deep sedation analgesia in the ICU. However, risk factors for delirium associated with propofol-based remain unclear. The study aimed to explore of propofol singled or combined sedation. This retrospective analyzed ICU patients who underwent surgery. were divided into three groups: (P), + midazolam (PM), dexmedetomidine (PD) groups. We utilized univariate multivariate logistic regression identify delirium. Delirium...