The final envelopment of most herpesviruses occurs at Golgi or post-Golgi compartments, such as the trans network (TGN); however, site human herpesvirus 6 (HHV-6) is uncertain. In this study, we found novel pathways for HHV-6 assembly and release from T cells that differed, in part, those alphaherpesviruses. Electron microscopy showed late infection, HHV-6-infected were larger than uninfected contained many newly formed multivesicular body (MVB)-like compartments included small vesicles....

Human herpesvirus-6B (HHV-6B) is a T lymphotropic β-herpesvirus that clearly distinct from human herpesvirus-6A (HHV-6A) according to molecular biological features. The International Committee on Taxonomy of Viruses recently classified HHV-6B as separate species. primary infection causes exanthem subitum and sometimes associated with severe encephalopathy. More than 90% the general population infected during childhood, virus remains throughout life latent infection. reactivation encephalitis...

Human herpesvirus type 6A (HHV-6A) can cause a series of immune and neurological diseases, the establishment sensitive biosensor for rapid detection HHV-6A is great significance public health safety. Herein, bis-tridentate iridium complex (BisLT-Ir-NHC) comprising N-heterocyclic carbene (NHC) ligand as novel kind efficient ECL luminophore has been unprecedently reported. Based on its excellent properties, new ECL-based sandwich immunosensor to detect virus was successfully constructed by...

An intriguing area of research has demonstrated the ability extracellular vesicles (EVs) as biological vehicles for microRNAs (miRNAs) transfer. Mesenchymal stem cells (MSCs) produce large amounts EVs. Rat models ischemia/reperfusion (I/R) were established to explore expression profile thioredoxin-interacting protein (TXNIP), which was then knocked-down investigate its effects on myocardial remodeling, followed by detection infarction size (MIS), collagen volume fraction (CVF) and...

Purpose: This study aimed to investigate whether long noncoding RNA (lncRNA) LINC00467 could regulate proliferative and invasive abilities of glioma cells via p53 DNA methyltransferase 1 (DNMT1), so as participate in the occurrence progression glioma. Methods: expression was analyzed by GEPIA database cell lines detected qRT-PCR. The regulatory effects on proliferative, capacities cycle were conducted CCK-8 EdU assays, transwell assay flow cytometry, respectively. binding conditions between...

Human herpesvirus 6 (HHV-6) is a T-cell-tropic betaherpesvirus. A glycoprotein (g) complex that unique to HHV-6, gH/gL/gQ1/gQ2, viral ligand for its cellular receptor, human CD46. However, whether formation or one component of the required CD46 binding and how transported in cells are open questions. Furthermore, HHV-6-infected gQ1 protein modified with N-linked glycans expressed two forms different molecular masses: an 80-kDa form (gQ1-80K) 74-kDa (gQ1-74K). Only gQ1-80K, but not gQ1-74K,...

Human herpesvirus 6 (HHV-6) is an important immunosuppressive and immunomodulatory virus worldwide. However, whether how HHV-6 infection influences the metabolic machinery of host cell to provide energy biosynthetic resources for propagation remains unknown. In this study, we identified that HHV-6A promotes glucose metabolism in infected T cells, resulting elevated glycolytic activity with increase uptake, consumption lactate secretion. Furthermore, explored mechanisms involved...

Three iridium( iii ) tetrazolato complexes have been used in antibacterial, biofilm removal and for other bioactivities the first time.

ABSTRACT Human herpesvirus 6 (HHV-6) is a T cell-tropic betaherpesvirus. HHV-6 can be classified into two variants, HHV-6A and HHV-6B, based on differences in their genetic, antigenic, growth characteristics cell tropisms. The function of HHV-6B should analyzed more its life cycle, as than 90% people have the antibodies for but not HHV-6A. It has been shown that cellular receptor human CD46 viral ligand envelope glycoprotein complex gH/gL/gQ1/gQ2; however, receptor-ligand pair used by still...

ABSTRACT Human herpesvirus 6 (HHV-6) is a T-cell-tropic betaherpesvirus. HHV-6 can be classified into two variants, variant A (HHV-6A) and HHV-6B, based on genetic, antigenic, cell tropisms, although the homology of their entire genomic sequences nearly 90%. The HHV-6A glycoprotein complex gH/gL/gQ1/gQ2 viral ligand that binds to cellular receptor human CD46. Because gH has 94.3% amino acid identity between here we examined whether from one could complement its loss in other. Recently,...

Based on genetic and antigenic differences their cell tropism, human herpes virus-6 (HHV-6) has been classified into two variants, HHV-6A HHV-6B. Recently, these variants were re-classified as different species. The glycoprotein complex, gH/gL/gQ1/gQ2 binds to its cellular receptor, CD46; however, the corresponding complex in HHV-6B rarely CD46. To determine which viral molecules HHV-6A-CD46 binding, each molecule of (i.e., gH, gL, gQ1, or gQ2) was replaced with molecule, ability protein be...

Recently, we identified a novel receptor, CD134, which interacts with the human herpesvirus 6B (HHV-6B) glycoprotein (g)H/gL/gQ1/gQ2 complex and plays key role in entry of HHV-6B into target cells. However, details interaction between gH/gL/gQ1/gQ2 CD134 were unknown. In this study, cysteine-rich domain (CRD), CDR2, that is critical for binding to infection. Furthermore, found expression gQ1 gQ2 subunits was sufficient binding, different from 6A (HHV-6A) its CD46. Finally, region function....

The human herpesvirus-6 (HHV-6) infection induces cell-cycle arrest. In this study, we found that the HHV-6-encoded U14 protein induced arrest at G2/M phase via an association with cellular EDD, a mediator of DNA-damage signal transduction. early HHV-6 infection, colocalized EDD dots in nucleus, and similar colocalization was also observed cells transfected expression vector. When carboxyl-terminal region deleted, no observed, percentage decreased relative to expressing wild-type U14,...

We identified two key amino acid residues within human CD134 (hCD134) that are required for its interaction with herpesvirus 6B (HHV-6B) and HHV-6B entry into cells. One of the (K79) allows access ligand to hCD134. Murine (mCD134) functioned as an receptor when these were replaced homologous residues. This study identifies both receptor-ligand species-specific determinants hCD134 essential entry.

Our previous studies indicate that long noncoding RNA (lncRNA) LINC00467 can act as an oncogene to participate in the malignant progression of glioma, but underlying molecular mechanism remains be studied further. This study aimed explore biological role LINC00467/miR-339-3p/ inositol hexakisphosphate kinase 2 (IP6K2) regulatory axis glioma. The Cancer Genome Atlas (TCGA), Oncomine databases and reverse transcription‑quantitative PCR (RT‑qPCR) were used analyze IP6K2 expression RT-PCR, EdU...

In general, enveloped viruses are highly dependent on their lipid envelope for entry into host cells. Here, we demonstrated that during the course of virus maturation, a significant proportion human herpesvirus 6 (HHV-6) proteins were selectively concentrated in detergent-resistant glycosphingolipid- and cholesterol-rich membranes (rafts) HHV-6-infected addition, ganglioside GM1, which is known to partition preferentially rafts, was detected purified virions, along with viral glycoproteins,...

Balò's concentric sclerosis (BCS) is a rare primary demyelinating disease of central nervous system (CNS) and considered to be variant multiple (MS). It characterized by severe, rapidly evolving course with CNS lesions consisting rings demyelination alternating myelination in the white matter. We report patient BCS from mainland China diagnosed on magnetic resonance imaging (MRI) findings. In addition two contrast enhanced open ring lesions. MRI also showed small matter both hemispheres. The...