A5086076274- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Neurological Disease Mechanisms and Treatments
- Mesenchymal stem cell research
- Acute Ischemic Stroke Management
- Cerebrovascular and Carotid Artery Diseases
- Immune cells in cancer
- Photoreceptor and optogenetics research
- Angiogenesis and VEGF in Cancer
- MicroRNA in disease regulation
- Barrier Structure and Function Studies
- Axon Guidance and Neuronal Signaling
- Target Tracking and Data Fusion in Sensor Networks
- Advanced X-ray Imaging Techniques
- Cancer-related molecular mechanisms research
- Intracranial Aneurysms: Treatment and Complications
- Optical Imaging and Spectroscopy Techniques
- Infrared Target Detection Methodologies
- Neuroscience and Neuropharmacology Research
- Intracerebral and Subarachnoid Hemorrhage Research
- Circular RNAs in diseases
- Moyamoya disease diagnosis and treatment
- Customer Service Quality and Loyalty
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
Shanghai Jiao Tong University
2015-2024
Shanghai Sixth People's Hospital
2024
Ruijin Hospital
2010-2022
Fudan University
2021
Second Military Medical University
2021
Renji Hospital
2021
Changhai Hospital
2021
Zhongshan Hospital
2021
University Research Co (United States)
2021
AVIC Optronics (China)
2014-2018
Metformin, a widely used hypoglycemic drug, reduces stroke incidence and alleviates chronic inflammation in clinical trials. However, the effect of metformin ischemic is unclear. Here, we investigated on mice further explored possible underlying mechanisms.Ninety-eight adult male CD-1 underwent 90-minute transient middle cerebral artery occlusion (tMCAO). Metformin (200 mg/kg) was administrated for up to 14 days. Neurobehavioral outcomes, brain infarct volume, inflammatory factors,...
Abstract Rationale: Cerebral ischemia upregulates aquaporin-4 expression, increases blood-brain barrier (BBB) permeability, and induces brain edema. Mesenchymal stem cells (MSCs) can repress inflammatory cytokines show great potential for ischemic stroke therapy. However, the effect of MSCs regarding protection ischemia-induced BBB break down is unknown. Objective: We test whether therapy protects integrity explore molecular mechanisms on integrity. Methods Results: Two hundred twenty-eight...
MicroRNA-29b (miR-29b) is involved in regulating ischemia process, but the molecular mechanism unclear. In this work, we explored function of miR-29b cerebral ischemia. The level white blood cells was evaluated patients and mice after ischemic stroke. Brain infarct volume National Institute Health stroke scale (NIHSS) scores were analyzed to determine relationship between expression severity aquaporin-4 (AQP4) further studied mice. We found that significantly downregulated (P < 0.05)....
Background and Purpose— Blood-brain barrier (BBB) disruption is a critical pathological feature after stroke. MicroRNA-126 (miR-126) maintains BBB integrity by regulating endothelial cell function during development. However, the role of miR-126-3p -5p in stroke unclear. Here, we investigated whether overexpression regulates cerebral ischemia. Methods— A lentivirus carrying genes encoding or was stereotactically injected into adult male Institute Cancer Research mouse brains (n=36)....
Patients with diabetes suffer the higher risk of dementia and underlying pathological mechanism cognitive dysfunction in is not fully understood. In this study, we explore whether impairment diabetic rat associated increased blood brain barrier (BBB) permeability change inflammatory cytokine. Experimental rats were induced by single intraperitoneal injection streptozotocin (STZ). Cognitive function was evaluated Morris water maze normal rats, respectively. The spatial acquisition trials...
Neural stem cells (NSCs) show therapeutic potential for ischemia in young-adult animals. However, the effect of aging on NSC therapy is largely unknown. In this work, NSCs were transplanted into aged (24-month-old) and (3-month-old) rats at 1 day after stroke. Infarct volume neurobehavioral outcomes examined. The number differentiated was compared ischemic angiogenesis neurogenesis also determined. We found that developed larger infarcts than ( P<0.05). outcome worse comparing with rats....
Although mammalian retinal ganglion cells (RGCs) normally cannot regenerate axons nor survive after optic nerve injury, this failure is partially reversed by inducing sterile inflammation in the eye. Infiltrative myeloid express axogenic protein oncomodulin (Ocm) but additional, as-yet-unidentified, factors are also required. We show here that infiltrative macrophages stromal cell–derived factor 1 (SDF1, CXCL12), which plays a central role regard. Among many growth tested culture, only SDF1...
Recent studies have demonstrated that the depletion of Regulatory T cells (Tregs) inhibits neural progenitor cell migration after brain ischemia. However, whether Tregs affect stem/progenitor proliferation is unclear. We explored effect on neurogenesis in subventricular zone were isolated and activated vitro. Adult male C57BL/6 mice underwent 60 minutes transient middle cerebral artery occlusion (tMCAO). Then (1x105) injected into left lateral ventricle normal ischemic mouse brain....
Background and Purpose— Acute interventions of stroke are often challenged by a narrow treatment window. In this study, we explore treatments in the postacute phase with wider windows opportunity. We investigated effects stromal cell–derived factor (SDF-1α) neurovascular recovery during downstream signaling pathways, underlying SDF-1α–mediated recovery. Methods— Adult male Institute Cancer Research (ICR) mice underwent middle cerebral artery occlusion. One week after occlusion, animals...
Studies demonstrate that microRNA-126 plays a critical role in promoting angiogenesis. However, its effects on angiogenesis following ischemic stroke are unclear. Here, we explored the effect of microRNA-126-3p and microRNA-126-5p neurogenesis after brain ischemia. We demonstrated both microRNA (miRNA)-126-3p increased proliferation, migration, tube formation human umbilical vein endothelial cells (HUVECs) compared with scrambled miRNA control (p < 0.05). Transferring into mouse middle...
Macrophages are involved in demyelination many brain diseases. However, the role of macrophages recovery phase ischemic is unknown. The present study aims to explore injury and tissue repair following a 90-min transient middle cerebral artery occlusion mice.Clodronate liposomes were injected into mice deplete periphery macrophages. These subsequently underwent occlusion. F4/80(+) CD68(+) cells examined mouse spleen confirm macrophage depletion at 14 days after Modified neurological severity...
Ischemic stroke causes a multitude of brain damage. Neurovascular injury and myelin sheath degradation are two manifestations ischemic Therapeutic strategies aiming only at repairing the neural components or vessels cannot efficiently restore neurological function. Endothelial progenitor cells (EPCs) have advantages both promoting angiogenesis secreting trophic factors that would promote neurogenesis. Chemokine cxcl12 gene therapy has also been shown to angiogenesis, neurogenesis,...
Dl-3-N-butylphthalide (NBP) is approved in China for the treatment of ischemic stroke. Previous studies have shown that NBP promotes recovery after stroke via multiple mechanisms. However, effect on vascular function and thrombosis remains unclear. Here, we aim to study using a rat model transient middle cerebral artery occlusion (MCAO) state-of-the-art high-resolution synchrotron radiation angiography. Eighty SD rats underwent MCAO surgery. (90 mg/kg) was administrated daily by gavage....
Abstract Three‐dimensional (3D) bioprinting is driving significant innovations in biomedicine over recent years. Under certain scenarios such as intraoperative bioprinting, the bioinks used should exhibit not only cyto/biocompatibility but also adhesiveness wet conditions. Herein, an adhesive bioink composed of gelatin methacryloyl, gelatin, methacrylated hyaluronic acid, and skin secretion Andrias davidianus designed. The exhibits favorable cohesion to allow faithful extrusion conditions,...
The relationship between circulating microRNA-223 and pathogenesis of acute ischemic stroke is unknown. Here we investigated the roles possible targets in human within first 72 hours. Blood samples were collected from patients hours after cerebral ischemia (n = 79) compared with healthy control 75). level downstream factors including insulin-like growth factor-1, factor-1 receptor interleukin-6 was examined by ELISA assay. NIHSS scores, TOAST subtypes, infarct volume analyzed respectively....
The intraluminal suture technique for producing middle cerebral artery occlusion in rodents is the most commonly used method modeling focal ischemia associated with clinical ischemic stroke. Synchrotron radiation angiography may provide a novel solution to directly monitor success of occlusion.Twenty adult Sprague-Dawley rats models were prepared randomly different head silicone coating. In vivo imaging was performed at beam line BL13W1, Shanghai Radiation Facility, Shanghai,...
Damage of oligodendrocytes after ischemia has negative impact on white matter integrity and neuronal function. In this work, we explore whether Netrin-1 (NT-1) overexpression facilitates repairing remodeling. Adult CD-1 mice received stereotactic injection adeno-associated virus carrying NT-1 gene (AAV-NT-1). One week transfer, underwent 60 minutes middle cerebral artery occlusion. The effect neural function was evaluated by neurobehavioral tests. Proliferated oligodendrocyte progenitor...
Previous studies revealed that curcumin is neuroprotective in diseases of the central nervous system such as cerebral ischemia and traumatic brain injury. However, effect on intracerebral hemorrhage remains unclear. We, therefore, investigated pre-clinical treatment neurological outcomes following hemorrhage, using a mouse model. Intracerebral was induced by autologous blood injection into right basal ganglia. Curcumin (150 mg/kg) administered 15 min after hemorrhage. Grid walk scores were...
Blood-brain barrier impairment is a major indicator of endothelial dysfunction in diabetes. Studies showed that progenitor cell (EPC) transplantation promoted angiogenesis and improved function recovery after hind limb ischemia diabetic mice. The effect EPC on blood-brain integrity cerebral animals unknown. aim this study to explore the EPCs were isolated by density gradient centrifugation characterized flow cytometry immunostaining. Diabetes was induced adult male C57BL/6 mice single...
Abstract Remyelination is an important repair process after ischemic stroke-induced white matter injury. It often fails because of the insufficient recruitment oligodendrocyte progenitor cells (OPCs) to demyelinated site or inefficient differentiation OPCs oligodendrocytes. We investigated whether CXCL12 gene therapy promoted remyelination middle cerebral artery occlusion in adult mice. The results showed that at 1 week ischemia could protect myelin sheath integrity perifocal region,...