A5101426997- Reproductive tract infections research
- Virus-based gene therapy research
- Cancer Research and Treatments
- Pneumonia and Respiratory Infections
- Syphilis Diagnosis and Treatment
- CAR-T cell therapy research
- Bacterial Identification and Susceptibility Testing
- Bacterial Infections and Vaccines
- Patient Safety and Medication Errors
- Streptococcal Infections and Treatments
- Antibiotic Resistance in Bacteria
- Veterinary Oncology Research
- Nanoparticles: synthesis and applications
- Neuroscience and Neuropharmacology Research
- Proteoglycans and glycosaminoglycans research
- Medical Malpractice and Liability Issues
- Cervical Cancer and HPV Research
- Urinary Tract Infections Management
- Herpesvirus Infections and Treatments
- Prostate Cancer Treatment and Research
- Respiratory viral infections research
- Parasitic Infections and Diagnostics
- Pneumocystis jirovecii pneumonia detection and treatment
- Genital Health and Disease
- Amoebic Infections and Treatments
Universidad Alfonso X el Sabio
2008-2025
Universidad de Oviedo
2015-2024
Fernández-Vega Ophthalmological Institute
2016-2024
Instituto de Investigación Sanitaria del Principado de Asturias
2018-2024
Central University Hospital of Asturias
2015-2024
Universidade São Judas Tadeu
2024
Misión Biológica de Galicia
2023
Universidad de Oriente
2022
Fundación Seimc-Gesida
2018-2021
University of Chile
1970-2020
ABSTRACT Three strains of Lactobacillus , identified as acidophilus gasseri and jensenii were selected from among 70 isolates the vaginas healthy premenopausal women for properties relevant to mucosal colonization or antagonism. All three self-aggregated adhered epithelial vaginal cells, displacing well-known pathogens, such G. vaginalis inhibiting growth in vitro Escherichia coli Streptococcus agalactiae . The surface components involved self-aggregation appeared be proteins L. lipoproteins...
ABSTRACT Pneumolysin (PLY) is an important virulence factor of Streptococcus pneumoniae . We examined the ability three murine monoclonal antibodies (MAbs) to PLY (PLY-4, PLY-5, and PLY-7) affect course pneumococcal pneumonia in mice. The intravenous administration PLY-4 PLY-7 protected mice from lethal effect purified toxin. Mice treated with before intranasal inoculation S. type 2 survived longer (median survival time, 100 h) than did untreated animals 60 ( P < 0.0001). median time for...
Intranasal inoculation of Streptococcus pneumoniae D39 serotype 2 causes fatal pneumonia in mice. The cytotoxic and inflammatory properties pneumolysin (PLY) have been implicated the pathogenesis pneumococcal pneumonia. To examine role PLY this experimental model we performed ELISA assays for quantification. distribution patterns apoptosis were established by immunohistochemical detection PLY, caspase-9 activity TUNEL assay on tissue sections from mice lungs at various times, results...
Abstract Background There are no accurate data regarding the real prevalence of Chlamydia trachomatis infection in Spain. Our aim was to determine C. infections and risk factors for acquiring them among 1,048 young (15–24 years old) inhabitants Laviana. Methods The study completed period between 1 st November 2010 31 December 2011. We conducted a capture strategy whole population, instead only sample group, with schools, local health centre, by post phone as last resort. design based on...
Purpose: Keratoconus is a heterogeneous disease associated with range of pathologies, including disorders that involve proteoglycans (PGs). Some PG alterations, mainly in keratan sulfate (KS), occur keratoconus. In this article, we studied the differential expression genes encoding PGs cells isolated from keratoconus patients and healthy controls, as well corneal sections. Methods: Human central tissue was obtained cadaver donors undergoing penetrating keratoplasty surgery. A transcriptomic...
Abstract Dogs with spontaneous tumors treated in veterinary hospitals offer an excellent opportunity for studying immunotherapies, including oncolytic viruses. Oncolytic viruses have advanced into the clinic as intratumorally administered therapeutic; however, intravenous delivery has been hindered by neutralization blood. To circumvent this hurdle, mesenchymal stem cells used a "Trojan horse." Here, we present treatment of 27 canine patients cancer infected ICOCAV17, adenovirus. No...
We have produced a panel of monoclonal antibodies to pneumolysin, the membrane-damaging toxin from Streptococcus pneumoniae. used these identify three regions sequence that are involved in lytic mechanism this toxin. Two sites probably form cell binding site Antibodies third inhibit action but not cells. This is engaged oligomerization process formation pores membranes. epitopes also present related perfringolysin O.
Quinolone susceptibility was analyzed in 17 clinical isolates of Corynebacterium striatum and 9 strains amycolatum by the E-test method Mueller-Hinton agar plates. The C. ATCC 6940 strain used as a control strain. amplified quinolone resistance determining regions gyrA genes were characterized. Four vitro quinolone-resistant mutants selected analyzed. Both vivo showed high levels fluoroquinolone with double mutation leading to an amino acid change positions 87 91 or 88 (unusual mutation)...
To assess the frequency, severity, and preventability of adverse events (AEs) detected using Global Trigger Tool (GTT) in an acute geriatric hospital.A 6-year retrospective study.An urban Spanish teaching hospital 200 beds.Ten randomly selected clinical records were chosen every fortnight from January 2007 to December 2012 (1,440 records, 240 per year).Triggers, AEs, Index National Coordinating Council for Medication Error Reporting Prevention (NCC MERP) categories Likert scale evaluate...
Oral tumors (squamous cell carcinoma, malignant melanoma, and fibrosarcoma) represent 6-7% of all canine cancers. Given that these have a high local recurrence rate metastatic potential, conventional therapies suboptimal response rates, leading to poor patient outcomes. Here, we report the use intratumoral virus-like particles from cowpea mosaic virus (CPMV) in four patients with recurrent oral lymph node metastasis. All were nonresponders chemotherapy had mild initial CPMV immunotherapy...
Owing to the limited validity of clinical data on treatment prostate cancer (PCa) and bone metastases, biochemical markers are a promising tool for predicting survival, disease progression skeletal-related events (SREs) in these patients. The aim this study was evaluate predictive capacity turnover mortality risk, SREs patients with PCa metastases undergoing zoledronic acid (ZA). This an observational, prospective multicenter which ninety-eight were included. Patients treated ZA (4 mg every...
Lower respiratory infections are among the top ten causes of death worldwide. Since pathogen to cell adhesion is a crucial step in infection progress, blocking interaction between eukaryotic receptors and bacterial ligands may enable pathogenesis process be stopped. Cell surface glycosaminoglycans (GAGs) known mediators diverse bacteria different types, making it interest examine their involvement attachment various pathogenic lung cells, including epithelial cells fibroblasts.The function...
Abstract Background The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, the magnitude observed effect is dependent on multiple, not fully understood, aspects, particle shape being one most important. In this article, we conduct a study recently described type AgNP:...
Thiol‐activated cytolysins share a conserved hydrophobic, Trp‐rich undecapeptide that is suggested to be involved in membrane binding and intercalation. The neutralizing monoclonal antibody PLY‐5 recognizes all members of this toxin family peptide mapping assigned its epitope the motif. This inhibited toxins host cell membranes was no longer available for when preformed toxin/membrane complex tested. These results confirm model cytolysin by structural data.
Anaerobic bacteremia (116 cases) represented 5.4% of the total cases in Hospital Nuestra Señora de Covadonga Oviedo, Spain, during a five-year period (1981–1985). Microbiologic data for all 116 and clinical 63 patients were analyzed. A 129 isolates identified as gram-negative bacilli (45.7%), grampositive (38.0%), gram-positive cocci (14.0%), (2.3%). Bacteroidesfragilis Clostridium perfringens most frequently occurring species. polymicrobial infection was detected 21 patients. The relevant...