A5008550312- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Ovarian cancer diagnosis and treatment
- Lung Cancer Treatments and Mutations
- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- Veterinary Oncology Research
- Immunotherapy and Immune Responses
- Estrogen and related hormone effects
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- HER2/EGFR in Cancer Research
- RNA modifications and cancer
- Growth Hormone and Insulin-like Growth Factors
- Breast Cancer Treatment Studies
- Endometrial and Cervical Cancer Treatments
- Cancer Genomics and Diagnostics
- Sarcoma Diagnosis and Treatment
- Cancer-related gene regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Microtubule and mitosis dynamics
- Colorectal Cancer Treatments and Studies
- Genomic variations and chromosomal abnormalities
- MicroRNA in disease regulation
Dartmouth College
2015-2025
Montefiore Medical Center
2010-2023
Albert Einstein College of Medicine
2010-2023
Dartmouth Cancer Center
2022
Dartmouth–Hitchcock Medical Center
2016-2022
Oklahoma State University Medical Center
2020
Oklahoma State University Center for Health Sciences
2020
Consejo Nacional de Investigaciones Científicas y Técnicas
1995-2018
Universidad de Morón
2018
University of New Mexico
2005-2016
Abstract Recurrent karyotypic abnormalities are a characteristic feature of cervical cancer (CC) cells, which may result in deregulated expression important genes that contribute to tumor initiation and progression. To examine the role gain long arm chromosome 20 (20q), one common chromosomal gains CC, we evaluated CC at various stages progression using single nucleotide polymorphism (SNP) array, gene profiling, fluorescence situ hybridization (FISH) analyses. This analysis revealed copy...
Chemotherapy remains the standard of care for most cancers worldwide, however development chemoresistance due to presence drug-effluxing ATP binding cassette (ABC) transporters a significant problem. The safe and effective means overcome is critical achieving durable remissions in many cancer patients. We have investigated energetic demands ABC context metabolic adaptations chemoresistant cells. Here we show that use mitochondrial-derived as source energy efflux drugs out further demonstrate...
ABSTRACT Integration of human papillomavirus type 16 (HPV-16) into the host DNA has been proposed as a potential marker cervical neoplastic progression. In this study, quantitative real-time PCR (qRT-PCR) was used to examine physical status HPV-16 in 126 carcinoma situ and 92 invasive cancers. Based on criteria applied results from qRT-PCR assay, characterized cases episomal (61.9%), mixed (i.e., integrated; 29.4%), integrated (8.7%) forms. cancer samples, similarly (39.1%), (45.7%), (15.2%)...
Cervical cancer (CC), a leading cause of cancer-related deaths in women worldwide, has been causally linked to genital human papillomavirus (HPV) infection. Although host genetic alterations have identified, molecular basis CC development is still poorly understood. We examined the role promoter hypermethylation, an epigenetic alteration that associated with silencing tumor suppressor genes cancer, by studying 16 gene promoters 90 cases. found high frequency methylation CDH1, DAPK, RARB, and...
Abstract Cervical cancer (CC) cells exhibit complex karyotypic alterations, which is consistent with deregulation of numerous critical genes in its formation and progression. To characterize this complexity at the molecular level, we used cDNA array comparative genomic hybridization (aCGH) to analyze 29 CC cases identified a number over represented deleted genes. The aCGH analysis revealed least 17 recurrent amplicons six common regions deletions. These contain several known tumor‐associated...
Abstract Patients with advanced stage invasive cervical cancer (CC) exhibit highly complex genomic alterations and respond poorly to conventional treatment protocols. In our efforts understand the molecular genetic basis of CC, we examined role Fanconi Anemia (FA)-BRCA pathway. Here, show that FANCF gene is disrupted by either promoter hypermethylation and/or deregulated expression in a majority CC. Inhibition DNA methylation histone deacetylases induces re-expression CC cell lines....
The presence and benefit of a radiation therapy-associated immune reaction is great interest as the overall in cancer immunotherapy expands. pathological assessment irradiated tumors rarely demonstrates consistent or inflammatory response. More recent information, primarily associated with "abscopal effect", suggests subtle radiation-based systemic response may be more common have therapeutic potential than previously believed. However, to value, stimulatory therapy (RT) will clearly need...
Abstract Background Cervical Cancer (CC) exhibits highly complex genomic alterations. These include hemizygous deletions at 4p15.3, 10q24, 5q35, 3p12.3, and 11q24, the chromosomal sites of Slit-Robo pathway genes. However, no candidate tumor suppressor genes these regions have been identified so far. Slit family secreted proteins modulates chemokine-induced cell migration distinct somatic types. mediate their effect by binding to its receptor Roundabout (Robo). shown be inactivated promoter...
Carcinoma of uterine cervix is the second most common cancers among women worldwide. Combined radiation and chemotherapy choice treatment for advanced stages disease. The prognosis poor, with a five-year survival rate ranging from about 20–65%, depending on stage Therefore, genetic characterization essential understanding biology clinical heterogeneity in cervical cancer (CC). We used genome-wide screening method – comparative genomic hybridization (CGH) to identify DNA copy number changes...
Abstract Background Copy number gains and amplifications are characteristic feature of cervical cancer (CC) genomes for which the underlying mechanisms unclear. These changes may possess oncogenic properties by deregulating tumor-related genes. Gain short arm chromosome 5 (5p) is most frequent karyotypic change in CC. Methods To examine role 5p gain, we performed a combination single nucleotide polymorphism (SNP) array, fluorescence situ hybridization (FISH), gene expression analyses on...
This study was undertaken to examine the role of insulin-like growth factor (IGF) signaling pathway in response ovarian cancer cells Taxol and evaluate significance this human epithelial tumors.The effect treatment on AKT activation A2780 carcinoma evaluated using antibodies specific for phospho-AKT. To drug-resistant phenotype, we developed a Taxol-resistant cell line, HEY-T30, derived from HEY cells. IGF2 expression measured by real-time PCR. A type 1 IGF receptor (IGF1R) inhibitor,...
Inflammatory mammary cancer (IMC), the counterpart of human inflammatory breast (IBC), is deadliest form canine tumors. IMC patients lack specific therapy and have poor outcomes. This proof-of-principle preclinical study evaluated efficacy, safety, effect on survival neoadjuvant intratumoral (in situ) empty cowpea mosaic virus (eCPMV) immunotherapy in companion dogs diagnosed with IMC.Ten IMC-bearing were enrolled study. Five received medical therapy, five weekly situ eCPMV (0.2-0.4 mg per...
Abstract Cervical cancer (CC) is the second most common in women. Currently, no tractable molecular‐based therapeutic targets exist for patients with invasive CC and predictive markers of risk assessment progression precancerous lesions are identified. New molecular insights into pathogenesis urgently needed. Towards this goal, we first determined copy number alterations chromosome 4 then examined role PCDH10 mapped to 4q28 as a candidate tumor suppressor gene. We identified monosomy 47% 81...
Inflammatory breast cancer (IBC) is a highly angiogenic disease; thus, antiangiogenic therapy should result in clinical response. However, trials have demonstrated only modest responses, and the reasons for these outcomes remain unknown. Therefore, purpose of this retrospective study was to determine prognostic value protein levels vascular endothelial growth factor (VEGF-A), one main targets therapy, its receptors (VEGF-R1 -R2) IBC tumor specimens.Specimens from normal tissues were obtained...
The lack of optimal models to evaluate novel agents is delaying the development effective immunotherapies against human breast cancer (BC). In this prospective open label study, we applied neoadjuvant intratumoral immunotherapy with empty cowpea mosaic virus-like particles (eCPMV) 11 companion dogs diagnosed canine mammary (CMC), a spontaneous tumor resembling BC. We found that two eCPMV injections resulted in reduction injected tumors all patients and noninjected located ipsilateral...