A5019275732- Glaucoma and retinal disorders
- Receptor Mechanisms and Signaling
- Neurological Disorders and Treatments
- Retinal Development and Disorders
- Nanoparticle-Based Drug Delivery
- Neuroscience and Neuropharmacology Research
- Pharmacological Receptor Mechanisms and Effects
- Neuropeptides and Animal Physiology
- Nitric Oxide and Endothelin Effects
- Retinopathy of Prematurity Studies
- Bacterial Infections and Vaccines
- Hedgehog Signaling Pathway Studies
- Protist diversity and phylogeny
- Medical Research and Treatments
- Apelin-related biomedical research
- Bladder and Urothelial Cancer Treatments
- Advanced Drug Delivery Systems
- Genomics and Chromatin Dynamics
- Coral and Marine Ecosystems Studies
- Advanced Decision-Making Techniques
- Marine and coastal ecosystems
- Epigenetics and DNA Methylation
- Cerebral Venous Sinus Thrombosis
- Urinary Tract Infections Management
- Retinal Diseases and Treatments
City University of Hong Kong
2021-2025
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2025
Fourth Affiliated Hospital of China Medical University
2019-2023
China Medical University
2012-2023
City University of Hong Kong, Shenzhen Research Institute
2021-2023
University of Hong Kong
2022
Chinese University of Hong Kong
2022
Qingdao University
2022
Smith-Kettlewell Eye Research Institute
2012-2018
South China University of Technology
2018
Abstract Breast cancer is a heterogeneous disease, affecting over 3.5 million women worldwide, yet the functional role of cis-regulatory elements including super-enhancers in different breast subtypes remains poorly characterized. Triple-negative (TNBC) an aggressive subtype with poor prognosis. Here we apply integrated epigenomic and transcriptomic profiling to uncover super-enhancer heterogeneity between subtypes, provide clinically relevant biological insights towards TNBC. Using...
L-lactate plays a critical role in learning and memory. Studies rats showed that administration of exogenous into the anterior cingulate cortex hippocampus (HPC) improved decision-making enhanced long-term memory formation, respectively. Although molecular mechanisms by which confers its beneficial effect are an active area investigations, one recent study found supplementation results mild reactive oxygen species burst induction pro-survival pathways. To further investigate changes induced...
Bacterial extracellular vesicles (EVs) are secreted by bacteria into the environment. Containing DNA, RNA and proteins, EVs implicated to mediate intercellular communications. The marine cyanobacterium Prochlorococcus , most abundant photosynthetic organism in ecosystems, has been shown generate continuously during cell growth. However, biogenesis functions of released remain largely unclear. Here, we isolated characterized MED4 culture. We found that majority elliptical enriched with...
This study examined the effect of H2O2 on TRPC6 channel and its underlying mechanisms using a heterologous expression system. In TRPC6-expressing HEK293T cells, significantly stimulated Ca2+ entry in dose-dependent manner. Electrophysiological experiments showed that increased open probability whole-cell currents. also evoked robust inward current A7r5 vascular smooth muscle which was nearly abolished by knockdown small interfering RNA. Catalase substantially attenuated arginine vasopressin...
Sigma-1 receptors (σ-1rs) exert neuroprotective effects on retinal ganglion cells (RGCs) both in vivo and vitro. This receptor has unique properties through its actions several voltage-gated ligand-gated channels. The purpose of this study was to investigate the role that σ-1rs play regulating cell calcium dynamics activated L-type Voltage Gated Calcium Channels (L-type VGCCs) purified RGCs. RGCs were isolated from P3-P7 Sprague–Dawley rats by sequential immunopanning using a Thy1.1...
Purpose.: Glaucoma is an optic neuropathy commonly associated with elevated intraocular pressure (IOP), leading to nerve head (ONH) cupping, axon loss, and apoptosis of retinal ganglion cells (RGCs), which could ultimately result in blindness. Brn3b a class-4 POU domain transcription factor that plays key role RGC development, outgrowth, pathfinding. Previous studies suggest decrease levels occurs animal models glaucoma. The goal this study was determine if adeno-associated virus...
Despite the rapid coral reef decline from climate change, molecular dynamics underlying environmental responses remain elusive. Filling this gap is vital to conservation. Here, we investigated seasonal proteomes of Platygyra carnosa, a stress-tolerant subtropical brain coral, using natural samples across wet and dry seasons with distinct conditions. Over 5,000 host proteins were profiled, revealing co-regulated modules related temperature, pH, dissolved oxygen, salinity, turbidity....
Abstract Leonurus japonicus houtt, a well‐known herb of traditional Chinese medicine, is widely used to treat gynaecological diseases. In this study, rapid and sensitive liquid chromatography with tandem mass spectrometry method for simultaneously quantifying leonurine stachydrine, the two main bioactive components in was developed validated. Plasma samples were prepared by protein precipitation acetonitrile separation Hewlett Packard XDB‐C8 column (150 × 4.6 mm, id, 5 μm) equipped gradient...
Highly pathogenic fowl adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), which characterized by pericardial effusion and hepatitis, one foremost causes economic losses to poultry industry over last 30 years. However, metabolic changes in cells response FAdV-4 infection remain unclear. In order understand interactions between host cell virus, we utilized ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry...
A simple and efficient method based on cloud‐point extraction combined with high‐performance liquid chromatography was developed validated for the determination of larotaxel in rat plasma. Nonionic surfactant Triton X‐114 chosen as solvent. Variable parameters affecting efficiency, example nature concentration surfactant, NaOH concentration, incubation temperature, time were evaluated optimized. Chromatographic separation accomplished a Diamonsil C 18 column (150 mm × 4.6 mm, 5 μm) using...
ABSTRACT Background In patients with chronic kidney disease (CKD), vascular calcification (VC) is common and associated a higher risk of all-cause mortality. Shh, one ligand for Hedgehog (Hh) signaling, participates in osteogenesis several cardiovascular diseases. However, it remains unclear whether Shh implicated the development VC. Methods Inorganic phosphorus 2.6 mM was used to induce smooth muscle cells (VSMCs) calcification. Mice were fed adenine diet supplement 1.2% Results decreased...
Papillary thyroid carcinoma (PTC) is often combined with other types of disease, such as Hashimoto's thyroiditis (HT), nodular goiter (NG), follicular adenoma (FA) and types.However, the function these diseases in PTC tumorigenesis development not well understood.In this research, 563 patients were recruited divided into two groups according to pathological diagnosis, namely simple (PTC+).Clinicopathological characteristics BRAF V600E mutation status compared between PTC+.Our data showed...
Surface biotinylation has been widely adapted in profiling the cellular proteome associated with plasma membrane. However, workflow is subject to interference from cytoplasmic biotin-associated proteins that compete for streptavidin-binding during purification. Here we established a bioorthogonal conjugation-assisted purification (BCAP) utilizes Staudinger chemoselective ligation label and isolate surface-associated while minimizing binding of endogenous proteins. Label-free quantitative...
Uropathogenic Escherichia coli (UPEC) are causative agent that causes urinary tract infections (UTIs) and the recent emergence of multidrug resistance (MDR) UPEC increases burden on community. Recent studies bacterial outer membrane vesicles (OMV) identified various factors including proteins, nucleic acids, small molecules which provided inter-cellular communication within population. However, components UPEC-specific OMVs their functional role remain unclear. Here, we systematically...
Stony corals form the foundation of coral reefs, which are prominent ecological and economic significance. A robust workflow for investigating proteome is essential in understanding biology. Here we investigated different preparative workflows characterized Platygyra carnosa, a common stony South China Sea. We found that combination bead homogenization with suspension trapping (S-Trap) preparation could yield more than 2700 proteins from samples. Annotation using P. carnosa transcriptome...
c-Jun, c-Jun N-terminal kinase(JNK) and endothelin B (ETB) receptor have been shown to contribute the pathogenesis of glaucoma. Previously, we reported that an increase CCAAT/enhancer binding protein β (C/EBPβ) immunohistostaining is associated with upregulation ETB within ganglion cell layer rats elevated intraocular pressure (IOP). In addition, both transcription factors regulate expression in human non-pigmented ciliary epithelial cells (HNPE). The current study addressed mechanisms by...
Microbial interactions including competition, mutualism, commensalism, parasitism, and predation, which can be triggered by nutrient acquisition chemical communication, are universal phenomena in the marine ecosystem. The may influence microbial population density, metabolism, even their environmental functions. Herein, we investigated interaction between a heterotrophic bicosoecid flagellate, Pseudobodo sp. (Bicoecea), dinoflagellate, Gambierdiscus balechii (Dinophyceae), is well-known...
The main pathological factor of cerebral infarction is atherosclerosis, which the process chronic inflammatory diseases such as vascular smooth muscle hyperplasia, cell infiltration, extracellular matrix increase, and thrombosis. At present, focus clinical treatment anti-platelet aggregation improving blood status, current research limited to symptoms only.To observe effect sodium ozagrel atorvastatin on type 2 diabetes patients with lacunar infarction.Eighty-two admitted our hospital from...