A5065943008- Cancer Research and Treatments
- Advanced Fluorescence Microscopy Techniques
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Cellular Mechanics and Interactions
- Optical Imaging and Spectroscopy Techniques
- Electrospun Nanofibers in Biomedical Applications
- Spine and Intervertebral Disc Pathology
- 3D Printing in Biomedical Research
- Photodynamic Therapy Research Studies
- Diabetic Foot Ulcer Assessment and Management
- Automotive and Human Injury Biomechanics
- Tendon Structure and Treatment
- Cell Image Analysis Techniques
- Bone fractures and treatments
- Photoacoustic and Ultrasonic Imaging
- AI in cancer detection
- Mitochondrial Function and Pathology
- Optical Coherence Tomography Applications
- Adipose Tissue and Metabolism
- Silk-based biomaterials and applications
- Aortic Disease and Treatment Approaches
- Nanoplatforms for cancer theranostics
University of Virginia
2025
University of Arkansas at Fayetteville
2015-2024
Tufts University
2011-2018
In-Q-Tel
2016
Brigham and Women's Hospital
2014
Harvard University
2014
University of Pennsylvania
2006-2011
The non-invasive high-resolution spatial mapping of cell metabolism within tissues could provide substantial advancements in assessing the efficacy stem therapy and understanding tissue development. Here, using two-photon excited fluorescence microscopy, we elucidate relationships among endogenous fluorescence, redox state differentiation human mesenchymal cells into adipogenic osteoblastic lineages. Using liquid chromatography/mass spectrometry quantitative PCR, evaluate sensitivity an...
Two-photon imaging provides noninvasive, label-free, quantitative assays of metabolic changes at the single-cell or tissue level.
Alterations in the balance between different metabolic pathways used to meet cellular bioenergetic and biosynthetic demands are considered hallmarks of cancer. Optical imaging relying on endogenous fluorescence has been as a noninvasive approach assess tissue changes during cancer development. However, quantitative correlations optical assessments with variations concentration relevant metabolites or specific that involved have lacking. In this study, we use high-resolution, depth-resolved...
Abstract Introduction Although stem cell therapy is a promising treatment for myocardial infarction, the minimal functional improvements observed clinically limit its widespread application. A need exists to maximize therapeutic potential of these cells by first understanding what factors within infarct microenvironment affect their ability regenerate necrotic tissue. In this study, we assessed both differentiation capacity and paracrine signaling as function extracellular matrix remodeling...
In vivo, label-free evaluation of mitochondrial function in human skin epithelia noninvasively differentiates healthy from cancerous tissues.
The development of prognostic indicators breast cancer metastatic risk could reduce the number patients receiving chemotherapy for tumors with low potential. Recent evidence points to a critical role cell metabolism in driving metastasis. Endogenous fluorescence intensity nicotinamide adenine dinucleotide (NADH) and flavin (FAD) can provide label-free method assessing metabolism. We report optical redox ratio FAD/(FAD + NADH) four isogenic triple-negative lines varying Under normoxic...
Abstract Chronic wounds are difficult to diagnose and characterize due a lack of quantitative biomarkers. Label-free multiphoton microscopy has emerged as useful imaging modality capable quantifying changes in cellular metabolism using an optical redox ratio FAD/(NADH+FAD) autofluorescence. However, the utility for long-term vivo monitoring tissue not been robustly evaluated. In this study, we demonstrate how can be used monitor individual full-thickness skin vivo. 3D maps NADH fluorescence...
Excessive cervical facet capsular ligament stretch has been implicated as a cause of whiplash-associated disorders following rear-end impacts, but the pathophysiological mechanisms that produce chronic pain in these cases remain unclear. Using rat model C6–C7 joint capsule produces sustained mechanical hyperalgesia, presence neuronal hyperexcitability was characterized 7 days after loading. Extracellular recordings spinal dorsal horn activity between C6 and C8 (117 neurons) were obtained...
Defining fiber orientation at each voxel within a 3D biomedical image stack is potentially useful for variety of applications, including cancer, wound healing and tissue regeneration. Current methods are typically computationally intensive or inaccurate. Herein, we present weighted vector summation algorithm, which generalization previously reported 2D technique aimed quantifying collagen orientations simultaneously an stack. As result, voxel-wise information with 4° to 5° accuracy can be...
Abstract Degenerative skin diseases affect one third of individuals over the age sixty. Current therapies use various physical and chemical methods to rejuvenate skin; but since many tissue components including cells extracellular matrix, they may also induce significant side effects, such as scarring. Here we report on a new, non-invasive, non-thermal technique with pulsed electric fields. The fields destroy while simultaneously completely preserving matrix architecture releasing multiple...
Detecting the initiation of mechanical injury to biological tissue, and not just its ultimate failure, is critical a sensitive specific characterization tissue tolerance, development quantitative relationships between macro- microstructural responses, appropriate interpretation physiological responses loading. We have developed novel methodological approach detect onset spatial location structural damage in collagenous soft before visible rupture, via identification atypical regional...
Although the mechanical phenomena associated with preconditioning are well-established, underlying mechanisms responsible for this behavior still not fully understood. Using quantitative polarized light imaging, study assessed whether alters collagen fiber alignment of ligament tissue, and determined changes in organization reduced force stiffness observed during loading. Collagen maps facet capsular ligaments (n = 8) were generated before after 30 cycles cyclic tensile loading, vectors...
Defining fiber orientation at each pixel within a medical image has traditionally been computationally intensive and prone to systematic errors. A weighted vector summation algorithm capable of detecting simultaneously an is presented. As result, pixel-specific information with 2 deg 3 accuracy can be determined seconds, enabling the practical use pixel-wise data for characterizing structural anisotropy. This analysis technique applicability potential diagnostic utility variety modalities,...
Background Mammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established important factors in For example, epithelial cells fail to form normal acinar structures vitro 3D gels that exceed the stiffness a mammary gland. Additionally, heterogeneity spatial distribution acini ducts within individual collagen suggests local organization...
Mitochondrial organization is often altered to accommodate cellular bioenergetic and biosynthetic demands. Changes in metabolism are a hallmark of number diseases, including cancer; however, the interdependence between mitochondrial metabolic function not well understood. Here, we present noninvasive, automated quantitative method assess three-dimensional (3D) tissues using exclusively endogenous two-photon excited fluorescence (TPEF) show that reflects alterations activities. Specifically,...
Abstract Understanding the organization and mechanical function of extracellular matrix (ECM) is critical for development therapeutic strategies that regulate wound healing following disease or injury. However, these relationships are challenging to elucidate during remodeling myocardial infarction (MI) due rapid changes in cellularity an inability characterize both ECM microstructure non-destructively. In this study, we overcome those challenges through whole organ decellularization...