Abstracts Bowel inflammation, impaired intestinal epithelial barrier (IEB), and gut dysbiosis could represent early events in Parkinson’s disease (PD). This study examined, a descriptive manner, the correlation among enteric α-synuclein, bowel impairments of IEB alterations bacteria transgenic (Tg) model PD before brain pathology. Human A53T α-synuclein Tg mice were sacrificed at 3, 6, 9 months age to evaluate concomitance impairments, bacterial metabolite during phases α-synucleinopathy....

The chemokine receptor CCR2 is a G protein-coupled that involved in many diseases characterized by chronic inflammation, and therefore large variety of small molecule antagonists has been developed. On the basis their chemical structures these can roughly be divided into two groups with most likely topographically distinct binding sites. aim current study was to identify site one such group ligands, exemplified three allosteric antagonists, CCR2-RA-[R], JNJ-27141491, SD-24. We first used...

Leptospira interrogans serogroup Icterohaemorrhagiae is the major infecting humans worldwide, and rodents dogs are most significant transmission sources in urban environments. Knowledge of prevalent serovars their maintenance hosts essential to understand epidemiology leptospirosis. In this study, 20 isolates were evaluated by pulsed-field gel electrophoresis (PFGE), variable number tandem-repeat analysis (VNTR), serotyping, determination antimicrobial resistance profile. Isolates,...

In patients with Parkinson's disease (PD), constipation is common, and it appears in a prodromal stage before the hallmark motor symptoms. The present study aimed to investigate whether Velusetrag, selective 5‑HT4 receptor agonist, may be suitable candidate improve intestinal motility mouse model of PD. Five months old PrP human A53T alpha-synuclein transgenic (Tg) mice, which display severe along decreased colonic cholinergic transmission already at 3 months, were treated daily drug for 4...

O objetivo do presente trabalho foi investigar a conveniência emprego de estirpes leptospiras autóctones isoladas no Brasil, na coleção antígenos da microtécnica soroaglutinação microscópica (SAM) aplicada leptospirose. Foram amostradas por 109 propriedades e 9820 bovinos, fêmeas em idade reprodutiva, distribuídos 85 municípios, dos Estados Goiás, Mato Grosso, Grosso Sul, Minas Gerais, Paraná, Rio Grande Santa Catarina São Paulo. Dos animais examinados, 5806 (59,12%) foram reagentes SAM para...

RESUMO O presente trabalho teve como objetivo avaliar o potencial zoonótico da população canina do Município de Santana Parnaíba, São Paulo, com relação à leptospirose. experimento foi conduzido durante a campanha vacinacão anti-rábica animal 1999, colheita 410 amostras soro canino e preenchimento um questionário pelos proprietários dos animais, para traçar perfil epidemiológico proceder análise estatística fatores risco. A leptospirose determinada através técnica soroaglutinação...

In swine and bovines, leptospirosis prevention control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency vaccines has been questioned. This work aimed to investigate potency five sold commercially in Brazil, challenging with one autochthonous strain Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, renal carriers Leptospira were identified among surviving by culture PCR. Of tested, only two proved...

With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract 137 female swine (40 sows 97 gilts) also urine samples from 22 were collected in a slaughterhouse State São Paulo, April 2003 to August 2004. Four isolates obtained animals that presented microagglutination test (MAT) titers ≥ 100 for serovar Pomona one was an animal negative by MAT which isolated reproductive tract. The presence leptospiral DNA investigated PCR, positive results found kidneys 11 females,...

We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with strains Leptospira serogroup Pomona: Fromm strain serovar Kennewicky, isolated from pigs in United States, and GR6 Pomona Brazil. Both were added aluminum hydroxide as an adjuvant. Experimental tested hamster potency test order to assess protection provided against disease establishment kidney infection. Controls polyvalent commercial vaccine outside Brazil, which...

Leptospirosis is a widespread systemic zoonosis, considered as reemerging in certain developing countries. Although the cross agglutinin absorption test still standard method for Leptospira identification, it presents several disadvantages. The aim of this study was to characterize spp. isolated from various hosts by genotyping and broth microdilution susceptibility testing an attempt differentiate species, serogroups serovars. Forty-seven isolates were studied. They previously serotyped,...

Leptospirosis is a widespread zoonosis caused by bacteria of the genus Leptospira. Rodents appear to be most important reservoirs infection. They contaminate environment and food can transmit pathogen when they are consumed carnivores. Capybara ( Hydrochaeris hydrochaeris ) efficient Leptospira, because in close contact with farm animals found semiurban areas, represent risk public health. We isolated five Leptospira strains from capybara kidneys Sao Paulo State, Brazil, 2001 typed them...

Endoplasmic reticulum (ER) dysfunction is important for alpha-synuclein (αS) acquired toxicity. When targeted to the ER in SH-SY5Y cells, transient or stable expression of αS resulted formation compact αS-positive structures a small subpopulation resembling inclusions. Thus, because limitations immunofluorescence, we developed set FRET biosensors (AFBs) able track conformation cells. In native conditions, i36, cell line αS, intermolecular AFBs, reporters which CFP YFP has been fused with...

With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract 137 female swine (40 sows 97 gilts) also urine samples from 22 were collected in a slaughterhouse State São Paulo, April 2003 to August 2004. Four isolates obtained animals that presented microagglutination test (MAT) titers > 100 for serovar Pomona one was an animal negative by MAT which isolated reproductive tract. The presence leptospiral DNA investigated PCR, positive results found kidneys 11 females,...

Introduction: Leptospira interrogans swine infection is a cause of serious economic loss and potential human health hazard. In Brazil, the most common serovars associated with infections are Pomona, Icterohaemorrhagie Tarassovi. Cross-reactions among failure infected animals to seroconvert may complicate interpretation serological tests. Molecular methods better discriminatory powers useful tools for leptospirosis characterization diagnosis. Methodology: This study evaluated nine L. isolates...

This study is aimed at relating social cognition in Huntington's Disease (HD) to plasma levels of the hormone oxytocin (OT). Indeed, HD patients commonly display reduced skills and OT involved bonding behavior improved recognition facial emotions. Twelve mild-symptomatic (stage II Shoulson & Fahn) 11 gender/age matched controls (healthy controls, HC), without concurrent psychiatric disorders, were investigated baseline (T₀) for through an extensive battery neuropsychological tests....

Leptospirosis presents a complex and dynamic epidemiology. Bovine leptospirosis has been described as major infectious disease impairing reproductive efficiency. Although infections by Leptospira interrogans, L. santarosai borgpetersenii are frequently reported in cattle, the presence of noguchii these animals should not be neglected. In this study, we describe serological (MAT) molecular characterization (rrs secY gene sequencing, multilocus sequence typing [MLST] pulsed-field gel...

elds. Early on M. Knebusch observed that this set up might be used to investigate theories of quadratic forms over certain classes (commutative, unitary) rings. This talk will present the main ideas and results such investigation, initiated by authors in 2004/2005, now published extenso [2]. Our deal with diagonal invertible coecients rings meeting some minimal conditions orderability. The approach is based an extension classical notion isometry preordered (p-rings) (A;T ), where 2...

Inactivation of leptospires in pools semen from three Holstein Friesian bulls, collected an artificial vagina, was investigated. Spermatic concentration adjusted egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, µg/mL); C (amoxicillin, D (ceptiofur sodium, E (amoxicillin µg/mL ceptiofur sodium µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into diluted semen. Recovery obtained modified...

Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" using serial dilution technique, in order evaluate percentage of recovery added microorganism. The selective found higher leptospiras minor losses samples due contamination opportunistic microorganisms than non-selective medium: 151/376 (40.0%) positive growth; 38/376 (10.0%) 58/376...

The Leptospira interrogans serogroup Australis is traditionally described as pathogenic for swine although animals usually present low serologic response and mild clinical signs. first isolation of a strain from swine, in Brazil, was recently described. Herein we the genomic characterization this (SU5) further comparison with serovars reference genomes available GenBank database. SU5 characterized sequence types previously isolated human presented new ST98 that thus far exclusively Brazilian...