A5067676296- Ovarian cancer diagnosis and treatment
- Cervical Cancer and HPV Research
- PARP inhibition in cancer therapy
- Global Cancer Incidence and Screening
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- Colorectal Cancer Screening and Detection
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Research in Social Sciences
- Ferroptosis and cancer prognosis
- AI in cancer detection
- Migration, Health and Trauma
- Immune cells in cancer
- Reproductive tract infections research
- BRCA gene mutations in cancer
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- Lung Cancer Treatments and Mutations
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Economic and Financial Impacts of Cancer
University of Helsinki
2017-2025
Helsinki University Hospital
2017-2025
University of Copenhagen
2025
Institute for Molecular Medicine Finland
2024
Finnish Cancer Registry
2010-2023
Cancer Society of Finland
2022
Precision Therapeutics (United States)
2017-2019
Kainuun keskussairaala
2013
Jorvi Hospital
2011
In-Q-Tel
2010
Background: The Nordic Cancer Registries are among the oldest population-based registries in world, with more than 60 years of complete coverage what is now a combined population 26 million. However, despite being source substantial number studies, there no published paper comparing different registries. Therefore, we did systematic review to identify similarities and dissimilarities Registries, which could possibly explain some differences cancer incidence rates across these...
Chemotherapy resistance is a critical contributor to cancer mortality and thus an urgent unmet challenge in oncology. To characterize chemotherapy processes high-grade serous ovarian cancer, we prospectively collected tissue samples before after analyzed their transcriptomic profiles at single-cell resolution. After removing patient-specific signals by novel analysis approach, PRIMUS, found consistent increase stress-associated cell state during chemotherapy, which was validated RNA situ...
Ovarian high-grade serous carcinoma (HGSC) is typically diagnosed at an advanced stage, with multiple genetically heterogeneous clones existing in the tumors long before therapeutic intervention. Herein we integrate clonal composition and topology using whole-genome sequencing data from 510 samples of 148 patients HGSC prospective, longitudinal, multiregion DECIDER study. Our results reveal three evolutionary states, which have distinct features genomics, pathways, morphological phenotypes,...
The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability fresh tumor material. Here, we describe a method for generation long-term expansion HGSC with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established cryopreserved material, demonstrating the feasibility using viably biobanked tissue organoid derivation. Genomic, histologic, single-cell transcriptomic analyses...
Attendance in screening is an important determinant of cervical cancer. Previous experience on high-risk human papillomavirus (hrHPV) DNA testing patient-obtained samples suggests a good effect among nonattendees screening. We assessed the effects self-sampling attendance Finnish program.Nonattendees after primary invitation one municipality (Espoo) were randomized to receive either kit (2,397 women) or extra (6,302 women). One fourth (1,315 reminder letter arm also received as third...
Abstract Introduction To evaluate predicting clinical factors for regression and progression of cervical intraepithelial neoplasia (CIN) grade 2 (CIN2) in young women during two years active surveillance. Material Methods This was a single‐center prospective observational cohort study. Women under 31 age giving written informed consent with histologically confirmed CIN2 were followed colposcopy, cytology, biopsies every 6 months up to 24 months. At baseline, HPV genotyping performed on...
Background Differences in cancer survival between the Nordic countries have previously been reported. The aim of this study was to examine whether these differences outcome remain, based on updated information from five national registers.Materials and methods data used for analysis NORDCAN database focusing nine common cancers diagnosed 1990–2016 Denmark, Finland, Iceland, Norway Sweden with maximum follow-up through 2017. Relative (RS) estimated at 1 5 years using flexible parametric RS...
Abstract Optimizing attendance and coverage of organized screening is needed to reduce cervical cancer incidence previous lower levels. In our study, all nonparticipants in 2008 Espoo, Finland were randomized receive a self‐sampling kit (1,130 women) or reminder letter (3,030 women). Effects on assessed according the self‐reported Pap smear history participants. Participation rate arm, 29.8%, was significantly higher than 26.2% (adjusted relative risk for participation 1.13). Total Espoo...
High attendance is essential to cervical cancer screening results. Attendance in the Finnish program currently at 70%, but extensive opportunistic occurs beside organized. A shift from organized imperative optimize costs and impact of minimize potential harms. We evaluated effect reminder letters (1st reminder) self-sampling test (2nd on attendance. The study population consisted 31,053 invitees 31 municipalities. 8,284 non-attendees after one invitation received a letter 4,536 further were...
Recent research on vaginal microbiota relies high throughput sequencing while microscopic methods have a long history in clinical use. We investigated the correspondence between findings of Pap smears and composition determined by next generation among 50 asymptomatic women. Both produced coherent results regarding distinction Lactobacillus-dominant versus mixed microbiota, reassuring gynaecologists for use smear or wet mount microscopy rapid evaluation bacteria as part diagnosis. Cytologic...
The severity of the COVID-19 pandemic and subsequent mitigation strategies have varied across Nordic countries. In a joint population-based effort, we compared patterns new cancer cases notifications between countries during 2020. We used pathology to registries in Denmark, Faroe Islands, Finland, Iceland, Norway Sweden determine monthly numbers malignant situ tumours from January December 2020 2019 (2017-2019 for Iceland Islands). per month based on unique individuals with notifications....
BackgroundSurvival of patients with colon and rectal cancer has improved in all Nordic countries during the past decades. The aim this study was to further assess survival trends by age at diagnosis present additional measures.MethodsData on cases diagnosed between 1990 2016 were obtained from NORDCAN database. Relative estimated using flexible parametric models. Both age-standardized age-specific measures for women men models, as well reference-adjusted crude probabilities death life-years...
Deficiency in homologous recombination (HR) repair of DNA damage is characteristic many high-grade serous ovarian cancers (HGSC). It imperative to identify patients with recombination-deficient (HRD) tumors as they are most likely benefit from platinum-based chemotherapy and PARP inhibitors (PARPi). Existing methods measure historical, not necessarily current HRD and/or require high tumor cell content, which achievable for patients. We set out develop a clinically feasible assay identifying...
To uncover the intricate, chemotherapy-induced spatiotemporal remodeling of tumor microenvironment, we conducted integrative spatial and molecular characterization 97 high-grade serous ovarian cancer (HGSC) samples collected before after chemotherapy. Using single-cell analyses, identify increasingly versatile immune cell states, which form spatiotemporally dynamic microcommunities at tumor-stroma interface. We demonstrate that chemotherapy triggers redistribution exhaustion CD8+ T cells due...
High coverage and attendance is essential for cervical cancer screening success. We investigated whether the previous positive experiences on increasing by self-sampling in Finland are sampler device dependent. All women identified to 2013 28 Finnish municipalities were randomised receive a lavage- (n = 6030) or brush type of 6045) case non-attendance after two invitation letters. Seven hundred seventy non-attending lavage group 734 received offer. Women's enquired with an enclosed...
Abstract High-grade extrauterine serous carcinoma (HGSC) is an aggressive tumor with high rates of recurrence, frequent chemotherapy resistance, and overall 5-year survival less than 50%. Beyond determining confirming the diagnosis itself, pathologist review histologic slides provides no prognostic or predictive information, which in sharp contrast to almost all other types. Deep-learning based image analysis has recently been able predict outcome and/or identify morphology-based...
Abstract Motivation Multiplexed imaging and single-cell analysis are increasingly applied to investigate the tissue spatial ecosystems in cancer other complex diseases. Accurate phenotyping based on marker combinations is a critical but challenging task due (i) low reproducibility across experiments with manual thresholding, and, (ii) labor-intensive ground-truth expert annotation required for learning-based methods. Results We developed Tribus, an interactive knowledge-based classifier...
Abstract High-grade serous carcinoma (HGSC) remains the deadliest gynecological cancer, mostly due to widespread metastases in abdominal cavities already at diagnosis. To improve therapy success, understanding metastasis routes and their molecular characteristics is crucial. Herein, we used >400 samples subjected whole-genome bisulfite (WGBS) RNA sequencing from 125 patients with HGSC, enrolled prospective, longitudinal, multi-region, observational DECIDER trial (NCT04846933). These...
Abstract Motivation Multiplexed imaging and single-cell analysis are increasingly applied to investigate the tissue spatial ecosystems in cancer other complex diseases. Accurate phenotyping based on marker combinations is a critical but challenging task due (i) low reproducibility across experiments with manual thresholding, and, (ii) labor-intensive ground-truth expert annotation required for learning-based methods. Results We developed Tribus, an interactive knowledge-based classifier...
Abstract Introduction: Among the gynecological malignancies, most prevalent and lethal is high-grade serous ovarian cancer (HGSC), accounting for 70-80% of mortality. Tumor evolution studies suggests that high molecular heterogeneity prevails long before treatment intervention, evincing patients would not benefit from a universal but rather personalized treatments. Here, we established prospective HGSC patient-derived tumor organoid biobank, where are testing lysosome-targeting FDA approved...
RNA in situ hybridization (RNA-ISH) is a powerful spatial transcriptomics technology to characterize target abundance and localization individual cells. This allows analysis of tumor heterogeneity expression localization, which are not readily obtainable through transcriptomic data analysis. RNA-ISH experiments produce large amounts there need for automated methods. Here we present QuantISH, comprehensive open-source image pipeline that quantifies marker expressions carcinoma, immune,...
Abstract Copy-number alterations (CNAs) are a hallmark of cancer and can regulate cell states via altered gene expression values. Herein, we have developed copy-number impact (CNI) analysis method that quantifies the degree to which value is impacted by CNAs leveraged this at pathway level. Our results show high CNA not necessarily reflected level, our capable detecting genes pathways whose activity strongly influenced CNAs. Furthermore, CNI enables unbiased categorization categories, such...
The world's population is aging rapidly. This study reports the burden of cancer in "oldest old" (aged ≥85 years) Finland, 1953-2017, and estimates age-specific rates older (65-99 for 1988-2017. Finnish Cancer Registry provided data on all diagnoses, deaths, other deaths patients Finland 1953-2017. Between 1953-1957 2013-2017, proportion incident cancers those aged years increased from 1.5% to 9.6% (597 15,360 new cases), more were diagnosed at ages than <50 years. incidence excess mortality...