A5047661725- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Ovarian function and disorders
- Kruppel-like factors research
- Circular RNAs in diseases
- Nuclear Receptors and Signaling
- Cancer-related molecular mechanisms research
- Assisted Reproductive Technology and Twin Pregnancy
- Estrogen and related hormone effects
- Pregnancy and preeclampsia studies
- Traditional Chinese Medicine Analysis
- Advanced Glycation End Products research
- Ectopic Pregnancy Diagnosis and Management
- Cholesterol and Lipid Metabolism
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Diet, Metabolism, and Disease
- Animal Diversity and Health Studies
- Sphingolipid Metabolism and Signaling
- Chromatography in Natural Products
- Gestational Trophoblastic Disease Studies
Nanjing Medical University
2012-2025
Nanjing Drum Tower Hospital
2015-2025
Renji Hospital
2024
Shanghai Jiao Tong University
2024
Xinjiang Academy of Agricultural Sciences
2024
Northwest A&F University
2020-2024
Wenzhou Medical University
2024
First Affiliated Hospital of Wenzhou Medical University
2024
Jiangsu Province Hospital
2022-2023
Nanjing University
2010-2023
Nitric oxide generated by endothelial nitric synthase (eNOS) plays an important role in maintaining cardiovascular homeostasis. Under various pathological conditions, abnormal expression of eNOS contributes to dysfunction and the development diseases. A variety stimuli has been reported decrease mainly through decreasing mRNA stability regulating binding several cytosolic proteins cis-acting sequences within 3' untranslated regions. However, detailed mechanisms remain elusive. Because...
Activin, a member of the transforming growth factor-β superfamily, promotes preantral follicles and proliferation granulosa cells. However, little is known about role microRNAs in activin-mediated cell proliferation. Here, we reported dose- time-dependent suppression microRNA-181a (miR-181a) expression by activin A mouse cells (mGC). Overexpression miR-181a mGC suppressed receptor IIA (acvr2a) binding to its 3′-untranslated region (3′-UTR), resulting down-regulation cyclin D2 proliferating...
Forkhead L2 (Foxl2) is expressed in ovarian granulosa cells and participates steroidogenesis by transcriptionally regulating target genes such as steroidogenic acute regulatory protein (StAR) CYP19A1. In this study, a direct link between microRNA‐133b (miR‐133b) Foxl2‐mediated estradiol release was established. miR‐133b involved follicle‐stimulating hormone (FSH)‐induced estrogen production. Luciferase assays confirmed that bound to the 3′ untranslated region (3′UTR) of Foxl2 mRNA....
Several studies have demonstrated that pre-treatment with long-acting Gonadotropin-Releasing Hormone agonists (GnRHa) can significantly enhance the clinical pregnancy rate among recurrent implantation failure (RIF) patients. Investigations also suggested GnRHa could ameliorate and live birth rates in polycystic ovary syndrome (PCOS) But there is a dearth of research on whether yields superior outcomes for RIF patients PCOS. The retrospective study enrolled 1602 under age 40 meeting criteria...
Objectives Methylglyoxal, a metabolite of the glycolysis pathway, may play an important role in development diabetes and hypertension, but exact mechanism has not been fully elucidated. The present study was designed to investigate whether methylglyoxal could directly induce insulin resistance salt sensitivity Sprague–Dawley rats. Methods Rats were allocated four groups: control (normal drinking water), 1% water, plus N-acetyl cysteine (NAC) (800 mg/kg per day), scavenger, or TM2002 (100...
MicroRNAs (miRNAs) are a class of 21‐ to 25‐nucleotide non‐coding RNAs, some which important gene regulators involved in folliculogenesis. In this study, we used CCK‐8, real‐time PCR and Western blot assays demonstrate that miR‐145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results luciferase reporter studied 3′‐untranslated region ACVRIB mRNA, these identified as direct target miR‐145. The ectopic expression reduced levels both mRNA protein also interfered...
Summary Objective Micro RNA s (mi s) are noncoding small that control gene expression at the posttranscriptional level. Some dysregulated mi have been shown to play important roles in epileptogenesis. The aim of this study was determine if miR‐199a‐5p regulates seizures and seizure damage by targeting antiapoptotic protein silent information regulator 1 ( SIRT 1). Methods Hippocampal levels miR‐199a‐5p, 1, acetylated p53 were quantified quantitative real‐time polymerase chain reaction...
Background:Homeobox A10 (HOXA10), a key transcription factor, plays critical role in endometrial receptivity by regulating the expression of downstream target genes, such as β3-integrin (ITGB3), but little is understood about mechanisms posttranslational modification HOXA10 during embryo implantation.
Estrogen synthesis is an important function of the mammalian ovary. plays roles in many biological processes, including follicular development, oocyte maturation and endometrial proliferation, dysfunctions estrogen contribute to development polycystic ovary syndrome premature ovarian failure. Classical signaling cascades triggered by follicle-stimulating hormone induce via upregulation Cyp19a1 granulosa cells (GCs). This study aimed determine effect microRNA-132 (miR-132) on estradiol GCs....
BackgroundRecurrent implantation failure (RIF) remains a critical and challenging problem in assisted reproductive technology mainly due to impaired decidualization. The endocytic transcytotic activity the endometrium are crucial for most representative gene is C-terminal Eps15 homology domain-containing 1 (EHD1), but whether EHD1-mediated function responsible embryo during decidualization unclear.MethodsA transcriptomic analysis was performed evaluate differentially expressed genes between...
This research aimed to explore the role of miR-135a-5p in head and neck squamous cell carcinoma (HNSCC) cells its influence on viability. Moreover, we compare effects miR-494 HNSCC, which was found repress
Objective . The aim of this study was to investigate the potential role IL-10 in regulating receptivity marker HOXA10 endometrium women with adenomyosis. Methods expression levels IL-10, HOXA-10, STAT3, and p-STAT3 adenomyosis controls were examined by means western blotting immunohistochemistry. protein Ishikawa cells treated rIL-10 blotting. attachment rate BeWo cell spheroids expressed as a percentage total number spheroids. Results group significantly lower than those control group,...
Abstract Decidualization is a complex process involving cellular proliferation and differentiation of the endometrial stroma required to establish support pregnancy. Dysregulated decidualization has been reported be critical cause recurrent implantation failure (RIF). In this study, we found that Activating transcription factor 3 (ATF3) expression was significantly downregulated in endometrium RIF patients. Knockdown ATF3 human stromal cells (hESCs) hampers decidualization, while...
How do NR4A receptors drive decidualization of human endometrial stromal cells (hESCs)? modulate by transcriptional activation FOXO1A, and in adenomyosis patients, the reduced expression eutopic endometrium may represent a novel mechanism to explain impaired subfertility. A close relationship between subfertility has been established. In cells, orphan nuclear receptor is regulator decidualization. Eutopic tissues hESCs from fertile controls (n = 56) patients 27) were collected for vitro...
The transformation of endometrium into decidua is essential for normal implantation the blastocyst. However, post-transcriptional regulation and miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression decidualized human endometrial stromal cell (hESC). In addition, investigated functional effect miR-181a on hESC vitro. Quantitative real-time PCR (qRT-PCR) was used to detect profile hESC. qRT-PCR, enzyme-linked fluorescent assay,...
HOXA10 has emerged as an important molecular marker of endometrial receptivity. Recurrent implantation failure (RIF) after in vitro fertilization-embryo transplantation (IVF-ET) treatment is associated with impaired receptivity, but the exact underlying mechanism this phenomenon remains elusive. Here we found that was modified by small ubiquitin like-modifier 1 (SUMO1) at evolutionarily conserved lysine 164 residue. Sumoylation inhibited protein stability and transcriptional activity without...
Successful embryo implantation requires the attachment of a blastocyst to receptive endometrial epithelium, which was disturbed in women with recurrent failure (RIF). Endometrial β3-integrin most important adhesion molecule contributing receptivity both humans and mice. Nur77 has been proven indispensable for fertility mice, here we explore role on embryo-epithelial potential treatment failure.The expression location Mst1 endometrium from fertile RIF patients were examined by IHC, qRT-PCR...
MicroRNAs (miRNAs) have been reported to play a key role in oncogenesis. Genetic variations miRNA processing genes and binding sites may affect the biogenesis of miRNA-mRNA interactions, hence promoting tumorigenesis. In present study, we hypothesized that potentially functional polymorphisms contribute head neck cancer (HNC) susceptibility. To test this hypothesis, genotyped three SNPs at (rs1057035 3′UTR DICER, rs3803012 RAN rs10773771 HIWI) with case-control study including 397 HNC cases...
One major precursor of carbonyl stress, methylglyoxal (MG), is elevated in the plasma chronic kidney disease (CKD) patients, and this contributes to progression vascular injury, hypertension renal injury diabetic nephropathy patients. This molecule induces salt-sensitive via a reactive oxygen species-mediated pathway. We examined role MG pathogenesis cardio–renal Dahl (Dahl S) rats, which rat model CKD. Nine-week-old S rats were fed 1% NaCl diet, was added their drinking water for up 12...