A5101562259- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Judicial and Constitutional Studies
- Ombudsman and Human Rights
- American Constitutional Law and Politics
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Mechanisms and Therapy
- Nanoparticle-Based Drug Delivery
- Viral Infections and Immunology Research
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Monoclonal and Polyclonal Antibodies Research
- China's Socioeconomic Reforms and Governance
- Conflict of Laws and Jurisdiction
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Cytokine Signaling Pathways and Interactions
- CAR-T cell therapy research
- Hong Kong and Taiwan Politics
- Cancer Research and Treatments
- Renal and related cancers
- Reproductive Biology and Fertility
- Chinese history and philosophy
Shanghai Jiao Tong University
2024
Shanghai Children's Hospital
2024
Children's Hospital of Fudan University
2019-2022
University of Maryland, Baltimore
2018-2021
Second Affiliated Hospital of Xi'an Jiaotong University
2021
Fudan University
2007-2021
Xinjiang Medical University
2021
Stem Cell Institute
2021
Beijing University of Technology
2017-2020
China Youth University for Political Sciences
2002-2019
Abstract Lentiviral vectors are attractive tools for liver-directed gene therapy because of their capacity stable expression and the lack preexisting immunity in most human subjects. However, use integrating may raise some concerns about potential risk insertional mutagenesis. Here we investigated liver transfer by integrase-defective lentiviral (IDLVs) containing an inactivating mutation integrase (D64V). Hepatocyte-targeted using IDLVs resulted sustained robust induction immune tolerance...
Human bone marrow (BM) mesenchymal stem/progenitor cells are potentially attractive targets for ex vivo gene therapy. The potential of lentiviral vectors transducing BM was examined using a self-inactivating vector that expressed the green fluorescent protein (GFP) from an internal cytomegalovirus (CMV) promoter. This compared with oncoretroviral expressing GFP CMV promoter or modified long-terminal repeat had been optimized long-term expression in stem cells. percentage GFP-positive...
Adult stem cells and induced pluripotent (iPS) hold great promise for regenerative medicine. The development of robust nonviral approaches cell gene transfer would facilitate functional studies potential clinical applications. We have previously generated hyperactive transposases derived from Sleeping Beauty, using an in vitro molecular evolution selection paradigm. now demonstrate that these resulted superior efficiencies expression mesenchymal muscle stem/progenitor cells, consistent with...
There is an urgent need for targeted biological therapies prostate cancer with greater efficacy and less toxicity, particularly metastatic disease, where current are not curative. Therapeutic adenoviral vectors or oncolytic adenoviruses offer the possibility of a competent, nontoxic therapeutic alternative cancer. However, free viral particles must be delivered locally, approach that does address they display poor tumor penetration. To fully exploit potential these vectors, we develop...
Multiple myeloma (MM), a clonal B cell malignancy characterized by the proliferation of plasma cells within bone marrow, is still an incurable disease, and therefore, finding new therapeutic targets urgently required. Although microRNA-137 (miR-137), which involved in variety cellular processes, has been reported to be under-expressed many types solid tumors, its role MM less known.In this study, target gene potential effect miR-137 were investigated. .The results showed significantly down...
Scope This study investigates the exosomal microRNA (miRNA) profiles of term and preterm breast milk, including most abundant differentially expressed (DE) miRNAs, their impact on neurodevelopment in infants. Methods Results Mature milk is collected from mothers Using high‐throughput sequencing subsequent data analysis, miRNA human (HBM) are acquired it found that let‐7 miR‐148 families miRNAs. Additionally, 23 upregulated 15 downregulated miRNAs identified. MiR‐3168 HBM exosome, exhibiting...
Abstract Breast cancer is the most prevalent malignancy in women, which remains untreatable once metastatic. The treatment of advanced breast restricted due to chemotherapy resistance. We previously investigated anti-cancer potential a tumor selective oncolytic adenovirus along with cisplatin three lung cells; A549, H292, and H661, found it very efficient. To our surprise, this virotherapy showed remarkable cytotoxicity chemo-resistant cells. Here, we extended investigation by using two...
Background: Enhancer of zeste homolog-2 (EZH2), a histone methyltransferase that regulates H3 methylation lysine27 (H3K27me3), is involved in the pathogenesis myelodysplastic syndrome (MDS). Targeting epigenetic regulators has been identified as potential treatment target MDS chemotherapy. Curcumin, natural compound extracted from turmeric, was found to possess wide range anticancer activities various tumors. Methods: This study designed investigate inhibitory effect and action mechanism...
Objective.Conditionally replicating adenoviruses (CRAds) have been proven potent oncolytic viruses in previous studies.They selectively replicate the tumor cells because of incorporated survivin promoter and ultimately lead to their killing with minimal side effects on normal tissue.Chemotherapy cisplatin is commonly employed for treating tumors, but its cytotoxic development resistance remained major concerns be dealt with.The aim this study was explore anticancer potential regulated CRAd...
Background:BRCA1/2 mutations are closely related to high lifetime risk of breast cancer (BC). The objective this study was identify the genes, regulators, and immune-associated patterns underlying disease pathology in BC with BRCA1/2 somatic their associations clinical traits. Methods: RNA sequencing data information from Cancer Genome Atlas (TCGA; N = 36 BRCA1-mutant BC; 49 BRCA2-mutant 117 BRCA1/2-wild-type samples) were used for discovery, which included consensus network analysis,...
Medulloblastoma is the most common malignant brain tumor in children. Despite improvement overall survival rate, it still lacks an effective targeted treatment strategy. The Janus family of cytoplasmic tyrosine kinases (JAKs) and Src kinases, upstream protein signal transducer activator transcription 3 (STAT3), play important roles medulloblastoma pathogenesis therefore represent potential therapeutic targets.
Persistent activation of signal transducer and activator transcription 3 (STAT3) is frequently reported in cancers plays important roles tumor progression. Therefore, directly targeting persistent STAT3 signaling an attractive cancer therapeutic strategy. The aim this study to test the inhibitory efficacy novel small molecule inhibitors, LLY17 LLL12B, combination with irradiation human medulloblastoma cells. Both LLL12B inhibit IL-6-induced phosphorylation Irradiation using 4 Gy alone...
Oligodendrocyte spheroids (OL-spheroids) containing oligodendrocytes and neurons provide an accessible system to dissect demyelinating diseases test therapeutic treatment. However, generation of human OL-spheroids is still technically challenging time-consuming until now. Here, we presented evidence that overexpression SOX10 OLIG2 (SO) in embryonic stem cells (hESCs)-derived ventral forebrain neural progenitors sufficient produce pre-oligodendrocytes (pre-OLs) mature (OLs) within 20-40 days....
A simple and automatic method for the determination of mercury ion by MEKC with on-column derivatisation LIF detection is described in present paper. In this method, solutions a nonfluorescent rhodamine derivative were injected individually mixed applying short voltage. Subsequently, ions reacted to produce strongly fluorescent product. The resulting product was then removed EOF micelles towards window detected detector. experimental conditions terms concentration injection volume ratios...
To develop a disease model for the human Alström Syndrome (AS), we used episomal reprogramming system and CRISPR/Cas9 technology to generate an induced pluripotent stem cell (iPSC) line with compound heterozygous patient mutation (ALMS1 c.3902C > A, c.6436C T) along isogenic gene-corrected control iPSC line. Both lines showed normal karyotype, expressed markers, differentiated into cells of three embryonic germ layer. These AS mutant will be great use in investigating mechanisms, drug...
Human brain development is a complex process involving neural proliferation, differentiation, and migration that are directed by many essential cellular factors drivers. Here, using the NetBID2 algorithm developing human RNA sequencing dataset, we identify synaptotagmin-like 3 (SYTL3) as one of top drivers early development. Interestingly, SYTL3 exhibits high activity but low expression in both developmental cortex embryonic stem cell (hESC)-derived neurons. Knockout (SYTL3-KO) neurons or...
Purpose: To delve into the related molecular mechanism of ACTL6A on non-small cell lung cancer (NSCLC) growth and apoptosis.Methods: Quantitative real-time polymerase chain reaction, immunohistochemical staining, western blot assays were employed to examine mRNA protein expression in four NSCLC line (NCI-H2170, LTEP-s, NCI-H1703, PC-9) normal (BEAS-2B). CCK-8 viability clone formation assay applied verify proliferation NCI-H2170 after knockdown ACTL6A. Flow cytometry check role apoptosis...
Human breast milk epithelial cells (BMECs) can be isolated and cultured with high purity. Induced pluripotent stem (iPSCs) were generated from BMECs Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using episomal system. Pluripotency of milk-derived iPSCs (BM-iPSCs) was confirmed by the expression markers immunocytochemistry spontaneous differentiation three germ layers in vitro teratoma formation assay vivo. Besides, iPSC lines displayed normal karyotype. Breast is a non-invasive easily...