A5011482853- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- T-cell and Retrovirus Studies
- Autoimmune and Inflammatory Disorders
- Chromosomal and Genetic Variations
- Dermatological and COVID-19 studies
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Viral-associated cancers and disorders
- Glycosylation and Glycoproteins Research
- Psoriasis: Treatment and Pathogenesis
- interferon and immune responses
- Cutaneous lymphoproliferative disorders research
- Systemic Lupus Erythematosus Research
- Parvovirus B19 Infection Studies
- Streptococcal Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Genetic and Kidney Cyst Diseases
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
Yale University
2020-2025
University of New Haven
2023
Vanderbilt University
2018
Indiana University School of Medicine
2012-2017
Indiana University – Purdue University Indianapolis
2012-2017
Riley Hospital for Children
2013
Indiana University Bloomington
2008-2009
Abstract Post-acute infection syndromes may develop after acute viral disease 1 . Infection with SARS-CoV-2 can result in the development of a post-acute syndrome known as long COVID. Individuals COVID frequently report unremitting fatigue, post-exertional malaise, and variety cognitive autonomic dysfunctions 2–4 However, biological processes that are associated persistence these symptoms unclear. Here 275 individuals or without were enrolled cross-sectional study included multidimensional...
COVID survivors frequently experience lingering neurological symptoms that resemble cancer-therapy-related cognitive impairment, a syndrome for which white matter microglial reactivity and consequent neural dysregulation is central. Here, we explored the neurobiological effects of respiratory SARS-CoV-2 infection found white-matter-selective in mice humans. Following mild mice, persistently impaired hippocampal neurogenesis, decreased oligodendrocytes, myelin loss were evident together with...
The emergence of SARS-CoV-2 variants with mutations in major neutralizing antibody-binding sites can affect humoral immunity induced by infection or vaccination1-6. Here we analysed the development anti-SARS-CoV-2 antibody and T cell responses individuals who were previously infected (recovered) uninfected (naive) received mRNA vaccines to SARS-CoV-2. While sustained higher titres than post-vaccination, latter reached comparable levels neutralization ancestral strain after second vaccine...
SARS-CoV-2 infection can result in the development of a constellation persistent sequelae following acute disease called post-acute COVID-19 (PASC) or Long COVID 1-3 . Individuals diagnosed with frequently report unremitting fatigue, post-exertional malaise, and variety cognitive autonomic dysfunctions ; however, basic biological mechanisms responsible for these debilitating symptoms are unclear. Here, 215 individuals were included an exploratory, cross-sectional study to perform...
Survivors of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection frequently experience lingering neurological symptoms, including impairment in attention, concentration, speed information processing and memory. This long-COVID cognitive syndrome shares many features with the cancer therapy-related (CRCI). Neuroinflammation, particularly microglial reactivity consequent dysregulation hippocampal neurogenesis oligodendrocyte lineage cells, is central to CRCI. We hypothesized...
Summary Strong sex differences in the frequencies and manifestations of Long COVID (LC) have been reported with females significantly more likely than males to present LC after acute SARS-CoV-2 infection 1–7 . However, whether immunological traits underlying differ between sexes, such explain differential symptomology is currently unknown. Here, we performed sex-based multi-dimensional immune-endocrine profiling 165 individuals 8 without an exploratory, cross-sectional study identify key...
Mitosis is controlled by a network of kinases and phosphatases. We screened library small interfering RNAs against genome-wide set phosphatases to comprehensively evaluate the role human in mitosis. found four candidate spindle checkpoint phosphatases, including tumor suppressor CDKN3. show that CDKN3 essential for normal mitosis G1/S transition. demonstrate subcellular localization changes throughout cell cycle. dephosphorylates threonine-161 CDC2 during mitotic exit we visualize...
Abstract Background Prior studies have shown the presence of immunohistochemical staining for SARS‐CoV‐2 spike protein (SP) in endothelial cells and eccrine epithelium acral perniosis classified as “COVID toes.” Yet, other been unable to detect RNA skin biopsies toes” by reverse‐transcriptase polymerase chain reaction testing. Objective In order address these apparently conflicting findings, we compared detection SP, through situ hybridization (ISH) vs immunohistochemistry (IHC), perniotic...
Significance Chilblain diagnoses have increased during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic and been attributed to viral infection a subsequent robust antiviral immune response. As result, providers managed these cases differently than idiopathic chilblains, which are associated with cold exposure. The relationship between chilblains SARS-CoV-2 infection, however, remains unclear as most patients do not test positive for SARS-CoV-2–specific PCR or...
Abstract Background Long COVID contributes to the global burden of disease. Proposed root cause hypotheses include persistence SARS-CoV-2 viral reservoir, autoimmunity, and reactivation latent herpesviruses. Patients have reported various changes in symptoms after COVID-19 vaccinations, leaving uncertainty about whether vaccine-induced immune responses may alleviate or worsen disease pathology. Methods In this prospective study, we evaluated vaccination 16 vaccine-naïve individuals with...
Abstract Background Discovering the functions of all genes is a central goal contemporary biomedical research. Despite considerable effort, we are still far from achieving this in any metazoan organism. Collectively, growing body high-throughput functional genomics data provides evidence gene function, but remains difficult to interpret. Results We constructed first network relationships for Drosophila melanogaster by integrating most available, comprehensive sets genetic interaction,...
Respiratory virus infections in humans cause a broad-spectrum of diseases that result substantial morbidity and mortality annually worldwide. To reduce the global burden respiratory viral diseases, preventative therapeutic interventions are accessible effective urgently needed, especially countries disproportionately affected. Repurposing generic medicine has potential to bring new treatments for infectious patients efficiently equitably. In this study, we found intranasal delivery neomycin,...
Abstract Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex heterogeneous multiorgan disease that can have severe impact on individuals' quality of life. Diagnosis ME/CFS based symptom presentation, and significant goal for the field to establish meaningful subtypes. The heterogeneity in literature suggests individuals living with may suffer from overlapping but different underlying pathophysiological mechanisms. We enrolled 40 participants 41 matched healthy control...
Cutaneous Lupus is an inflammatory skin disease causing highly morbid inflamed and hair loss. In order to investigate the pathophysiology of cutaneous lupus, we performed single-cell RNA spatial sequencing lesional non-lesional lupus compared healthy controls. Pathway enrichment analyses keratinocytes revealed elevated responses type I interferon, II tumor necrosis factor, apoptotic signaling. Detailed clustering demonstrated unique fibroblasts specific with likely roles in cell recruitment...
Acral inflammatory lesions that have some resemblance to idiopathic or autoimmune-associated perniosis (chilblains) been described in multiple countries during the COVID-19 pandemic.
Pyoderma gangrenosum (PG) is a sterile neutrophilic dermatosis manifesting as painful inflammatory plaques and ulcers, frequently associated with bowel disease, rheumatoid arthritis, myelodysplastic syndrome, acute myeloid leukemia.1 We present case of young woman novel mutation in NFkB1 who experienced common variable immunodeficiency (CVID) severe recurrent PG episodes.
The partially understood phosphoinositide signaling cascade regulates multiple aspects of cellular metabolism. Previous studies revealed that INPP5E, the inositol polyphosphate-5-phosphatase is mutated in developmental disorders Joubert and MORM syndromes, essential for function primary cilium maintenance balance nondividing cells. Here, we report INPP5E further contributes to homeostasis by regulating cell division. We found silencing or genetic knockout human murine cells impairs spindle...