Intra-articular injection of mesenchymal stem cells is a potential therapeutic strategy for cartilage protection and symptom relief osteoarthritis (OA). However, controlling chondrogenesis the implanted in articular cavity remains challenge. In this study, hydrogels containing different concentrations icariin were prepared by situ crosslinking hyaluronic acid Poloxamer 407. This injectable thermoresponsive hydrogel, as 3D cell culture system, showed good biocompatibility with chondrocytes...

Objectives: Metal micronutrients deficiency may be one of the risk factors for development osteoporosis. This study aimed to measure trace element contents in human bone tissue analyze relationship between and Design: A cross-sectional survey was performed on data from 51 elderly patients with proximal femoral fracture. Methods: The concentrations calcium, phosphorus, manganese, iron, copper, zinc samples fracture were determined by energy-dispersive X-ray fluorescence (EDX). Subjects...

Sarcopenia, a chronic degenerative condition associated with aging, is characterized by significant decline in muscle mass and strength. Puerarin, major active isoflavone extracted from Pueraria lobata, exhibits potent anti-inflammatory antioxidant properties. However, its therapeutic effects on sarcopenia remain unclear. Thus, the purpose of this study was to evaluate underlying molecular mechanisms puerarin ameliorating naturally aged mice. Twenty-month-old male C57BL/6 J mice were...

ABSTRACT Sarcopenia, marked by the loss of muscle mass and function, is a chronic condition that worsens with age. Currently, there are no effective drugs for its treatment. Puerarin, potent natural compound extracted from root Pueraria lobata , exhibits various pharmacological properties, including anti‐inflammatory, antioxidative, anti‐apoptotic effects. It remains unclear whether puerarin possesses anti‐muscle atrophy capabilities. This study aims to evaluate effectiveness in delaying...

In recent decades, the stromal cell-derived factor-l (SDF-1) and Gab1 have been investigated to be involved in oncogenesis. However, it is scarcely reported that SDF-1-Gab1 pathway mediates proliferation apoptosis human chondrosarcoma (CS). this study, we assessed expression of 90 CS solid tumors by immunohistochemistry, immunoblotting, qRT-PCR, then, some vitro assays were also applied cells treated with SDF-1. We observed overexpression was positively correlated lung metastasis recurrence,...

BACKGROUND:This study compared the efficacy and safety of 3 different anesthesia techniques used in total hip arthroplasty (THA). MATERIAL AND METHODS:We allocated 198 patients preparing to undertake THA into groups: general group (GA group, n=66), caudal epidural (CEA spinal-epidural (SEA n=66). We postoperative adverse effects occurring groups. The Visual Analog Scale (VAS) score, Minimum Mental State Examination (MMSE) β-amyloid (Aβ) expression were calculated determine on pain cognitive...

Background . The role of C‐reactive protein (CRP), an inflammatory marker, in the development sarcopenia remains uncertain. Methods This cross‐sectional research involved enrollment 207 patients, classified into two groups: 74 patients with and 133 without sarcopenia. Clinical data participants, including hand grip strength, walking speed, appendicular lean mass (ALM), calf circumference, were collected recorded. We evaluated extent to which CRP levels are associated risk using both...

Hip fractures significantly reduce the quality of life and mobility older adults. This study aimed to analyze correlation between volumetric bone mineral density (vBMD) in different regions proximal femur as measured by quantitative computed tomography (QCT) various subtypes hip fractures.

Percutaneous vertebroplasty (PVP) was recently performed for treating patients with osteoporotic vertebral compression fractures (OVCF). However, recompression of cemented vertebra significant height loss occurred in the after PVP observed during follow-up period. The purpose is to explore risk factors among several potential predictors treated bodies OVCF.A study 93 who had undergone between May 1, 2016, and March 2019, at Spine Center Huadong Hospital Affiliated Fudan University conducted....

Sarcopenia is a chronic disease characterized by an age-related decline in skeletal muscle mass and function, diagnosis challenging owing to the lack of clear "gold standard" assessment method. This study aimed at combining random forest (RF) artificial neural network (ANN) methods screen key potential biomarkers establish early sarcopenia diagnostic model. Three gene expression datasets were downloaded merged searching Gene Expression Omnibus (GEO) database. Differentially expressed genes...

The skeletal renin–angiotensin system (RAS) is involved in the progression of osteoporosis and active peptide within RAS, angiotensin II (ANG II), has deleterious effects on bones. This study was performed to investigate whether suppression rate-limiting step RAS cascade by renin inhibitor aliskiren a benefit trabecular bone osteoporotic mice. A postmenopausal model induced bilateral ovariectomy. ovariectomized (OVX) mice were treated with low (5 mg/kg) or high (25 dose for 6 weeks....

Objective. Blood vessels of bone are thought to influence osteogenesis bone. No clinical studies have determined whether angiogenesis is related mass and gene expression growth factors. We compared marrow endothelial progenitor cells (EPC), which control in postmenopausal women incurring fragility fracture, with osteoporosis or traumatic fracture normal (COM). Methods. Bone specimens were obtained from age-matched COM. Mononuclear isolated EPC detected by flow cytometry. The levels specific...

Introduction Trace metal elements may play a crucial role in bone mineralization and metabolism. However, the quantification of trace element concentrations human tissue has received little attention. Materials methods Bone samples were collected from 55 elderly patients (15 males 40 females) with intertrochanteric hip fractures. The calcium, phosphorus, manganese, iron, copper, zinc cortical zone, cancellous junction zone between determined by energy-dispersive X-ray fluorescence (EDX)....

Background. Sarcopenia is a common chronic disease characterized by age-related decline in skeletal muscle mass and function, the lack of diagnostic biomarkers makes community-based screening problematic. Methods. Three gene expression profiles related with sarcopenia were downloaded merged searching Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) eigengenes module dataset identified differential analysis weighted coexpression network (WGCNA), (CGs) defined as...

A domestic health record system is introduced. It includes a measuring instrument, memorizer, and two applications. Blood pressure, heart rate, body temperature blood oxygenation can be measured stored automatically. Other appended into memorizer manually. All records reviewed in an appropriate way. This provides means to memorize people's anamnesis routine physiological parameters, help making medical decisions.

Objective To construct lentiviral vectors targeting rat interleukin-1 receptor type I（IL- 1 RI） gene by RNA interference （RNAi） and to study its effects on chondrocytes in vitro. Methods Effec- tire sequence of siRNA IL-1RI was designed determined 239T cells were trans- fected with pGCL-GFP vector containing the inserted DNA sequence. The lentivirus effective concentration × 107 TU/ml obtained after supernatant 293T collected concentrated. recombinant used infect IL-l-stimulated previously...

The knee osteoarthritis(KOA)is a kind of degenerative disease, which is more common in the elderly.In recent years, according to Knee Uneven Settlement Theory, new treatment method for osteoarthritis by using proximal fibula osteotomy popularized many hospitals.In this article, we reported 1 case undergoing total arthroplasty after and reviewed relevant literatures. Key words: Knee prosthsis; Fibula; Osteotomy

Abstract Background: Load-induced pressure gradients (PG) result in interstitial fluid flow bone tissue, which is not only provides sufficient nutrient supply and metabolic pathway for the cells, but also enables cells to accept external mechanical signals. Methods: We calculate number distribution of canaliculus around osteocyte based on varying shape osteocyte, then use these calculated parameters other microstructure data tissue estimate anisotropy permeability lacunar–canalicular....