A5101579694- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Cancer therapeutics and mechanisms
- Bioactive Natural Diterpenoids Research
- Caveolin-1 and cellular processes
- Phytochemical compounds biological activities
- Lung Cancer Research Studies
- Sphingolipid Metabolism and Signaling
- Lipid metabolism and disorders
- Traditional and Medicinal Uses of Annonaceae
- Mechanisms of cancer metastasis
- Wnt/β-catenin signaling in development and cancer
- Protein Degradation and Inhibitors
- Corneal Surgery and Treatments
- Ferrocene Chemistry and Applications
- S100 Proteins and Annexins
- Genomics, phytochemicals, and oxidative stress
- Plant-derived Lignans Synthesis and Bioactivity
- Immunotherapy and Immune Responses
Affiliated Zhongshan Hospital of Dalian University
2022
Taizhou People's Hospital
2021
Nanjing University of Chinese Medicine
2021
Nantong University
2021
Yanbian University
2021
Yanbian University of Science and Technology
2021
Hubei University of Medicine
2021
Henan Cancer Hospital
2015-2020
Zhengzhou University
2015-2020
First Affiliated Hospital of Chinese PLA General Hospital
2020
Fenretinide [N-(4-hydroxyphenyl)retinamide (4-HPR)] is cytotoxic in many cancer cell types. Studies have shown that elevation of ceramide species plays a role 4-HPR cytotoxicity. To determine activity multidrug-resistant line as well to study metabolism, MCF-7/AdrR cells (redesignated NCI/ADR-RES) were treated with and sphingolipids analyzed. TLC analysis radiolabeled [3H]palmitic acid showed elicited dose-responsive increase radioactivity migrating the region chromatogram decrease...
Accumulating evidence has established that long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) is a tumor regulator in many cancers. Here, we aimed to investigate the possible function of lncRNA PVT1 esophageal carcinoma (EC) via targeting microRNA-145 (miR-145). Initially, microarray-based gene expression profiling EC was employed identify differentially expressed genes. Moreover, examined and cell line presenting with highest level selected for subsequent experiments....
The interaction of long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs has been implicated in various types cancers, including esophageal cancer (EC). current study aimed to investigate the role AGAP2-AS1/miR-195-5p/Fos-like antigen-1 (FOSL1) EC progression. expression AGAP2-AS1, miR-195-5p, FOSL1 tumor tissues isolated from patients cell lines was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), results which illustrated that AGAP2-AS1 were...
Cervical cancer is one of the most common cancers in females, with a high incidence and mortality around world. However, pathogenesis cervical not completely known. In present study, we investigated role miR-126-5p Bcl2l2 cells. First, expression was aberrantly downregulated human tumor tissues comparison normal tissues, as evaluated by RT-PCR. Consistently, levels were also significantly reduced cell lines when compared to epithelial Flow cytometric analysis showed that rate apoptosis cells...
MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR‐449c downregulation non‐small cell lung (NSCLC) compared with normal tissues was investigated this study. NSCLC proliferation invasion assays indicate transfection of miR‐449c plasmid inhibits the ability NCI‐H23 NCI‐H838 cells. In addition, overexpression could suppress growth vivo. Morever,...
Jesridonin, a small molecule obtained through the structural modification of Oridonin, has extensive antitumor activity. In this study, we evaluated both its in vitro activity cancer cell line EC109 and vivo effect on tumor xenografts nude mice. Apoptosis induced by Jesridonin was determined using an MTT assay, Annexin-V FITC assay Hoechest 33258 staining. via mitochondrial death receptor pathways were confirmed detecting regulation MDM2, p53, Bcl-2 family members activation caspase-3/-8/-9....
The aberrant expression of Wnt3 has linked to several types human malignancies. However, it is not known for its role in tumorigenesis colorectal cancer (CRC). Herein, we show that upregulated CRC tissues and essential the progression. Knockdown cells delayed tumor formation nude mouse xenografts through silencing canonical Wnt pathway glycolysis. We further found enhanced sensitivity cisplatin inducing apoptotic cell death. Taken together, demonstrates a novel clinical biomarker detection...
Mounting evidence has implicated the vital role of long noncoding RNAs (lncRNAs) in non-small cell lung cancer (NSCLC). This study aims to investigate mechanism lncRNA MEG8 on NSCLC progression. The mRNA expressions and miR-107 were examined tumor adjacent normal tissues from patients with by qRT-PCR. Lung epithelial BEAS-2B cells transfected overexpression plasmid, A549 H1299 or overexpression/knockdown plasmid detect function activity. proliferation, cycle changes, invasion migration was...
Abstract Bladder cancer is the most common neoplasm in urinary system. This study assesses arctigenin anti‐tumor activity human bladder T24 cells vitro and underlying molecular events. The flow cytometry analysis was used to detect cell‐cycle distribution apoptosis. Western blotting changes protein expression. data showed that treatment reduced viability of a dose‐ time‐dependent manner after with (10, 20, 40, 80, 100 μmol/L) for 24 hr 48 hr. Arctigenin clearly arrested tumor G1 phase cell...
Objective . To investigate the effects of β -Elemene ( -ELE) on proliferation, apoptosis, and topoisomerase I (TOPO I) II α ) expression activity human hepatocarcinoma HepG-2 cells. Methods After treatment with -ELE, morphological alterations cells were observed under an inverted microscope. Cell proliferation was assessed using MTT assay, cell cycles analyzed flow cytometry, apoptosis detected by Annexin V/PI staining. The TOPO Western blot techniques, their measured I-mediated, supercoiled...
Purpose: To investigate the effect and mechanism of Agrin on limbal stem cell proliferation corneal wound healing. Methods: Limbal cells were isolated treated with different concentrations Agrin. CCK-8 markers (Ki67 pH3) detected to evaluate numbers or proliferative potential cells. The epithelium model was induced by debridement central epithelial, effects epithelial healing rate determined. Results: promoted cultured in vitro increased expression level p63α rather than keratin 12....
Objective: To clarify the role of LINC00665 in ovarian cancer (OC) progression and possible mechanism.Methods: levels OC tissues cell lines were detected by qRT-PCR.The correlation between clinicopathologic characteristics patients was assessed.Biological functions phenotypes influenced examined CCK-8, colony formation Transwell assay.Dual-luciferase reporter assay RIP conducted to verify interaction its downstream target.Results: upregulated linked poor prognosis.Knockdown blocked malignant...
The lncRNA TUG1 promotes epithelial ovarian cancer cell proliferation and invasion via the WNT/β-catenin pathway Shankun Liu,1 Ying Qiang Lu,2 Xiao Zhou,2 Li Chen,2 Weifeng Liang3 1Department of Gynecology, Tai'an City Center Hospital, Tai'an, Shandong, People's Republic China; 2Department Obstetrics Affiliated Qingdao Hiser Hospital University (Qingdao Traditional Chinese Medicine), Qingdao, 3Department Qilu Shandong University, China Purpose: Epithelial (EOC) is among most common malignant...
Parkinson's disease (PD), a common multifactorial neurodegenerative disease, is characterized by irreversible loss of dopaminergic neurons in the substantia nigra. In‑depth study pathogenesis PD great importance. High‑mobility group AT‑hook 2 (HMGA2) has been proposed to be implicated with neuronal differentiation and impairment cognitive function. However, whether HMGA2 plays role rarely explored. In present study, N‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine (MPTP)‑treated mice models...
Pancreatic cancer (PC) is a leading cause of mortality worldwide. Hydroxysteroid dehydrogenase like protein 2 (HSDL2) overexpressed in variety malignant tumors and might be closely related to the development cancer. It also regulates different metabolism signaling pathways.The purpose this research was find HSDL2 expression levels investigate its underlying molecular mechanism PC.In present study, total 66 PC samples 54 normal tissues were used examine HSDL2. In order gain broader insight...
Glioma is the most common and aggressive of malignant brain tumours. MicroRNAs (miRNAs/miRs) are involved in tumour development various human cancers, including glioma. Therefore, miRNAs may have potential diagnostic, prognostic therapeutic values miR‑103 abnormally expressed cancer types. However, detailed expression pattern, biological functions underlying molecular mechanism glioma remain unclear. present study aimed to investigate expression, roles mechanisms Results demonstrated that...
Bone marrow mesenchymal stem cells (BMSCs) have the potential to transdifferentiate into cardiomyocyte‑like (CLCs) if an appropriate cardiac environment is provided. Insulin‑like growth factor‑1 (IGF‑1) plays important role in cell migration, survival and differentiation of BMSCs. However, effect IGF‑1 on cellular remains unclear. In present study, BMSCs were isolated from rat femurs tibias purified at passage 6 (P6). receptor (IGF‑1R) kinase inhibitor I‑OMe AG538 added detect could induce...
This aim of the present study was to observe effect high mobility group AT‑hook 2 (HMGA2) on proliferation and invasion ability ACHN renal cell carcinoma (RCC) cells. Human cells, an RCC line, HKC normal human tubular epithelial cells were cultured. HMGA2 small interfering (si)RNA, Mock‑siRNA their negative control designed synthesized. Subsequently, transiently transfected using RNA interference technology. Finally, mRNA protein expression levels detected reverse transcription-polymerase...
The present study aimed to investigate the effects of cinobufacini injection on proliferation and expression topoisomerases in human HepG‑2 hepatocarcinoma cells. cells were divided into a control group an experimental group, which 0.105, 0.21, 0.42 mg/l was injected. Cell assessed using 3‑(4,5‑dimethylthiazol‑2‑yl) ‑2,5‑diphenyltetrazolium bromide assay, levels apoptosis detected annexin V/propidium iodide staining cell cycles analyzed flow cytometric analysis. mRNA protein topoisomerase...
The aim of the present study was to examine microRNA (miRNA or miR)-455-5p expression in esophageal squamous cell carcinoma (ESCC) at tissue and cellular levels order elucidate its biological roles. A total 60 patients with ESCC were enrolled reverse transcription-quantitative polymerase chain reaction used measure miR-455-5p. Eca109 cells transfected miR-NC, miR-455-5p mimics inhibitor a Cell Counting Kit-8 assay assess proliferation. To investigate migration invasion abilities cells,...
Abstract Cancer multidrug resistance (MDR) is existence in stem cell‐like cancer cells characterized by stemness including high‐proliferation and self‐renewal. Programmed cell death 4 (PDCD4), as a proapoptotic gene, whether it engaged cisplatin still unknown. Here we showed that PDCD4 expressions Hela/DDP (cisplatin resistance) were lower than parental Hela cells. Moreover, the levels of drug genes typical markers markedly elevated In vivo, xenograft tumor assay confirmed knockdown...
To explore the role of high mobility group AT-hook 2 (HMGA2) in regulation cell cycle and apoptosis.The renal carcinoma line ACHN was transiently transfected with small interfering RNA to knock down expression HMGA2 gene. Cell analysis undertaken using flow cytometry. The mRNA protein levels HMGA2, E2F transcription factor 1 (E2F1), cyclin D1, dependent kinase 6 (CDK6), B-cell lymphoma-2 (Bcl-2), caspase-3 caspase-9 were analysed reverse quantitative real-time polymerase chain reaction...