Aerobic exercise training leads to a physiological, nonpathological left ventricular hypertrophy; however, the underlying biochemical and molecular mechanisms of physiological hypertrophy are unknown. The role microRNAs regulating classic novel cardiac renin-angiotensin (Ang) system was studied in trained rats assigned 3 groups: (1) sedentary; (2) swimming with protocol 1 (T1, moderate-volume training); (3) 2 (T2, high-volume training). Cardiac Ang I levels, Ang-converting enzyme (ACE)...

Background Accumulated evidence shows that the ACE-AngII-AT1 axis of renin-angiotensin system (RAS) is markedly activated in chronic heart failure (CHF). Recent studies provide information Angiotensin (Ang)-(1–7), a metabolite AngII, counteracts effects AngII. However, this balance between AngII and Ang-(1–7) still little understood CHF. We investigated exercise training on circulating skeletal muscle RAS ischemic model Methods/Main Results Male Wistar rats underwent left coronary artery...

Alterations in the balance between ANG II/ACE and 1–7/ACE2 II-dependent hypertension could reduce generation of 1–7 contribute further to increased intrarenal II. Upregulation collecting duct (CD) renin may lead II formation during hypertension, thus contributing this imbalance. We measured I, II, contents, angiotensin-converting enzyme (ACE) ACE2 gene expression, activity renal cortex medulla clipped kidneys (CK) nonclipped (NCK) 2K1C rats. After 3 wk unilateral clipping, systolic blood...

Background:Hyperactivity of the renin-angiotensin system (RAS) and functional deficits in hypertension are reduced after exercise training. We evaluate arteries, kidney plasma hypertensive rats sequential effects training on vascular angiotensinogen, Ang II (1-7) content.Methods Results:Spontaneously (SHR) Wistar-Kyoto (WKY) were trained or kept sedentary (S) for 3 months. After hemodynamic measurements (weeks 0, 1, 2, 4, 8 12), blood, arteries kidneys obtained to quantify angiotensin...

Chronic angiotensin II (ANG II) infusion for 1 or 2 wk leads to progressive hypertension and induces inward hypertrophic remodeling in preglomerular vessels, which is associated with increased renal vascular resistance (RVR) decreased glomerular perfusion. Considering the ability of vessels exhibit adaptive responses, present study was performed evaluate perfusion function after 6 ANG infusion. To address this study, male Wistar rats were submitted sham surgery (control) osmotic minipump...

Abstract Objective: To assess the effectiveness of ozonated water in disinfecting surfaces an Intensive Care Unit countryside Amazon. Methods: This is exploratory and descriptive research. select sample, three distinct areas were selected for each surface 15 care units assessed (air mattress, bed rails bedside table). Each area was subdivided into four subgroups: Control Group (CG), Hydrogen Peroxide (HPG), Hybrid (HDEO3G) Ozonated Water (O3G). These subjected to 12 adenosine triphosphate...

Resumo Objetivo: Avaliar a eficácia da água ozonizada na desinfecção de superfícies assistenciais em uma Unidade Terapia Intensiva no interior Amazônia. Métodos: Pesquisa exploratória, descritiva. Para seleção amostra, foram selecionadas três áreas distintas para cada superfície das 15 unidades avaliadas (colchão pneumático, grades laterais cama e mesa cabeceira). Cada área foi subdividida quatro subgrupos: Grupo Controle - GC, Peróxido Hidrogênio GPH, Híbrido GHDEO3 Água Ozonizada GO3),...

This study was conducted to evaluate time-dependent pharmacokinetic changes and drug interactions over the first 6 months after transplantation in kidney transplant recipients receiving tacrolimus (TAC), prednisone (PRED) mycophenolate mofetil (MMF) or sirolimus (SRL). Pharmacokinetic assessments were carried out at day 7 1, 3, TAC plus PRED with either MMF (2 g/day, n = 13) SRL (15 mg loading dose, 5 for days followed by 2 mg/day, 12). There no differences main demographic characteristics...

Overconsumption of fructose leads to metabolic syndrome as a result hypertension, insulin resistance, and hyperlipidemia. In this study, the renal function animals submitted high intake was analyzed from weaning adulthood using in-vivo ex-vivo methods, being compared with normal control group. We investigated in model role renin angiotensin system (RAS) kidney. The use perfused kidney 8-week treatment showed that caused cardiovascular alterations were consistent other studies. Moreover,...

Giardia spp. is a flagellate protozoan that presents two evolution forms, cysts and trophozoites. Cysts are resistant to chlorine, the most employed disinfectant agent in treatment of water. For this reason, new techniques for disinfection waters contain parasite necessary. This work evaluated efficiency by ozone ultrasound individually simultaneously upon wastewater. The data obtained showed after application, ozone, ultrasound, combined induced significant elimination cysts. Furthermore,...

The aim of this paper was to investigate the presence urinary 90 kDa N-domain ACE in a cohort population from Vitoria, Brazil, verify its association with essential hypertension since isoform could be possible genetic marker hypertension. Anthropometric, clinical, and laboratory parameters individuals were evaluated (n = 1150) blood pressure (BP) measured. study divided according isoforms urine as follows: 65/90/190, three 795), 90(+) (65/90) 186), 90(-) (65/190) 169) based on (+) or absence...

The acute effects of angiotensin-1–7 [ANG-(1–7)] on the reabsorptive bicarbonate flow (J[Formula: see text]) were evaluated using stationary microperfusion in vivo proximal tubules spontaneously hypertensive rats (SHR) and their normotensive controls, Wistar-Kyoto (WKY) rats, a microelectrode sensitive to H + . In WKY control J[Formula: text] was 2.40 ± 0.10 nmol·cm −2 ·s −1 ( n = 120); losartan (10 −7 M) or A779 −6 M, specific Mas antagonist), alone combination with losartan, decreased...

The purpose of this study was to perform a comparative biochemical analysis between conventional spectrophotometry and Raman spectroscopy, techniques used for diagnoses, on the urine healthy (CT) diabetic hypertensive patients (DM&HBP). Urine from 40 subjects (20 in CT group 20 DM&HBP group) examined dispersive spectrometer (an 830 nm excitation 350 mW power). mean spectra both groups showed significant difference peaks glucose; exploratory by principal component (PCA) identified spectral...

Renin-angiotensin system activation promotes oxidative stress and endothelial dysfunction. However, no previous study has examined the effects of renin inhibitor aliskiren, either alone or combined with angiotensin II type 1 antagonists on alterations induced by two-kidney, one-clip (2K1C) hypertension. We compared vascular aliskiren (50mg/kg/day), losartan (10mg/kg/day), both gavage for 4 weeks in 2K1C control rats. Treatment losartan, exerted similar antihypertensive effects. Aliskiren...

The aim of this study was to prevent female osteoporosis using strength training (ST), raloxifene (Ral) or a combination ST plus Ral during the natural aging process, specifically in periestropause period. For total 120 days, Wistar rats at 18-21 months age performed on ladder three times per week, and administered daily by gavage (1 mg/kg/day). Bone microarchitecture, areal bone mineral density, femoral neck, immunohistochemistry, osteoclast osteoblast surface were assessed. We found that...

Platelet transfusion therapy poses many challenges in veterinary clinical practice. Lack of readily available blood donors, short shelf-life, and inability to administer a sufficient number platelets meet dog's need are the major difficulties encountered. additive solutions already use at American European human banks, showing be realistic alternative. This study compares vitro platelet function plasma, Composol, or SSP+ during storage for 13 days. rich plasma-platelet concentrate with 35%...

The objective of this study is to identify whether oxytocin (OT) contributes the reduction osteopenia in femoral neck rats periestropause. Animals irregular estrous cycles received two NaCl injections (0.15 mol/L) or OT (134 μg/kg) over a 12-h interval, and after thirty-five days without treatments, biological sample collection was performed. group (Ot) demonstrated highest enzymatic activity alkaline phosphatase (p = 0.0138), lowest tartrate-resistant acid 0.0045), higher percentage compact...

The aim of this study was to investigate the association between urinary 90 kDa N-domain Angiotensin I-converting enzyme (ACE) form with C-reactive protein (CRP) and homocysteine plasma levels (Hcy), nitric oxide (NOu), endothelial function (EF) in normotensive subjects. Forty healthy subjects were evaluated through brachial Doppler US test response reactive hyperemia a panel blood tests determine CRP Hcy levels, NOu, ACE. They divided into groups according presence (ACE90+) or absence...

Angiotensin I-converting enzyme (ACE) plays a key role in the renin-angiotesin aldosterone cascade. We analysed secondary structure and structural organization of purified 65kDa N-domain ACE (nACE) from Wistar rat mesangial cells, 90 kDa nACE spontaneously hypertensive rats 130 somatic ACE. The C-terminal alignment 65 with revealed that former was truncated at Ser(482), sequence ended Pro(629). Protein's consisted predominantly alpha-helices. isoforms were most stable guanidine low pH,...

Background: The analysis of the complete blood count (CBC) patients with community-acquired pneumonia (CAP) is an essential practice both for diagnosing disease and evaluating patient's clinical evolution. It proposed in present study to analyze hematological alterations resulting from photobiostimulation using near-infrared light-emitting diodes (LEDs) CAP. Methods: This was a clinical, prospective, blinded, descriptive longitudinal that involved 21 undergoing CAP treatment who were divided...