Abstract We aimed to investigate the wound-healing, antioxidant, and anti-inflammatory effects of pterostilbene (PTS) on human gingival fibroblasts (GF). Different concentrations PTS were applied GFs cell viability was evaluated by MTT assay. stimulated lipopolysaccharide (LPS) study groups determined as LPS, LPS + 1 μM PTS, 10 control. The most effective in a wound-healing model, with counts wound area assessed at 0, 24, 48, 72 h. effect release interleukin-1β (IL-1β), tumor necrosis...

This study aimed to examine the effects of sevoflurane or isoflurane preconditioning on cerebral ischemia/reperfusion-induced inflammation, oxidative stress, and lipid peroxidation test hypothesis that underlining mechanism protective effect involves changes in apoptotic gene expression profiles an experimental model middle artery occlusion rats.Twenty-four adult male rats were randomly divided into 3 groups: control (n=8), (n=8). For preconditioning, these groups exposed 40% O2, 2%...

Background Autoimmunity plays an essential role in the pathogenesis of rheumatic heart disease (RHD); however, cellular mechanisms autoimmune response are unclear. Whereas T helper 17 (T H 17) and regulatory cells reg ) share a common differentiation pathway, they play opposite roles immune tolerance diseases. Although high 17/T ratio has been shown several diseases, no data available RHD. This study investigated balance between mitral valve (MVD). Methods Forty patients with MVD 23 control...

Abstract Thyroid-stimulating hormone (TSH), a central neuroendocrine mediator of the hypothalamus-pituitary-thyroid axis, has been shown to affect various aspects immunological development and function. To gain better understanding TSH involvement within mammalian immune system, expression distribution receptor (TSHr) studied by immunoprecipitation flow cytometric analyses. Using highly enriched populations B cells, T dendritic trace amounts TSHr were precipitated from cells whereas high...

Crimean-Congo hemorrhagic fever virus (CCHFV), a member of the genus Nairovirus family Bunyaviridae, causes severe disease in humans with high mortality rates. In Turkey, number patients CCHF has increased since 2002. Here, we aimed to treat CCHFV hyperimmunoglobulin. We prepared hyperimmunoglobulin product from 22 individuals who survived infection. A total 26 were enrolled into this study. For administration, Kubar Unit (KU) was defined. As standard therapeutic approach, 400 KU given each...

Smart materials have been attracting much attention because of their stimuli responsive nature. We synthesized biocompatible thermoresponsive crosslinked poly(ethylene glycol) methyl ether methacrylate (PEGMA)-co-vinyl pyrrolidone nanoparticles (PEGMA NPs) using disulfide-based crosslinker by surfactant-free emulsion polymerization method. Particle characterization studies were carried out dynamic light scattering, and scanning electron microscopy. Polymerization kinetics, effect initiator...

Objective We compared the effects of sevoflurane and isoflurane on systemic inflammation, sepsis-associated encephalopathy, memory impairment in a rat sepsis model cecal ligation puncture (CLP)-induced polymicrobial peritonitis. Methods Twenty-four rats were assigned to sham, CLP, CLP + sevoflurane, groups. At 72 hours after underwent behavior tests. Serum cytokines evaluated. Brain tissue samples collected for determination glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase;...

ABSTRACT The widespread use of wireless communication technologies has increased human exposure to radiofrequency electromagnetic fields (RF‐EMFs). Considering the brain's close proximity mobile phones and its entirely electrical transmission network, it emerges as organ most profoundly impacted by RF field. This study aims investigate potential effects radiation on cell viability, apoptosis, gene expressions in glioblastoma cells (U118‐MG) at different times (1, 24, 48 h). To achieve this,...

Interleukin 40 (IL-40) is a novel cytokine that has been associated with B lymphocytes, particularly IgA-secreting cells in gut mucosa. Considering mucosal association of IL-40, we aimed to determine serum IL-40 levels patients Covid-19. We compared concentrations healthy people both mild symptoms SARS-CoV-2 infection and pneumonia. was measured by ELISA. Serum IgA were tested nephelometry. For the first time, demonstrated increased significantly. The elevation related severity infection....

Immunoassays are simple, efficient and reliable means for testing patient samples in the modern hospital. Their specificity sensitivity have provided robust by which detection of analytes can be achieved with ease. The underlying principle antigen-antibody reactions forming a measurable complex exposes immunoassays to possible interference from proteins other reactants structural similarities antibodies or analytes. Accounting test its reliability, impact interferents both endogenous like...

It has been known for over a decade that sialidase (neuraminidase) treatment could substantially enhance the capacity of resting B cells to stimulate proliferation allogeneic and antigen specific, syngeneic T cells. Thus, cell-surface sialic acid was implicated as potential modulator immune cell interaction. However, little progress made in either identifying explicit roles this system or hypothesizing mechanisms explain "neuraminidase effect." Here we show first time surface on medium...

Bone marrow-derived mesenchymal stem cells are pluripotent that capable of differentiating into a variety cell types including neuronal cells, osteoblasts, chondrocytes, myocytes, and adipocytes. Despite recent advances in biology, neuroendocrine relations, particularly TSH interactions remain elusive. In this study, we investigated expression biological consequence receptor (TSHR) cultured human bone marrow. To the best our knowledge, demonstrated for first time expressed functional...

This study aimed to investigate the effects of anandamide or arachidonylethanolamide (AEA), an endogenous cannabinoid receptor agonist, on intraocular inflammation in endotoxin-induced uveitis (EIU) model rabbits.Forty New Zealand albino male rabbits were used (5 groups, 8 animals each). After establishment sufficient anesthesia, taken under surgery for intravitreal injections. A maximum amount 50 μL solution was injected into central vitreous with a 30-gauge needle. In control group,...

Background/Aim.The aim of the present study was to find out if there is an increase in expression pro-apoptotic Bax and reduction anti-apoptotic Blc-2A1 newborn intestines with necrotizing enterocolitis (NEC).Material Methods.We compared 8 consecutive patients undergoing bowel resection for NEC neonates intestinal ileal atresia.Histopathological evaluation tissue injury apoptosis performed by using light microscopic examination TUNEL method.The mRNA level apoptotic (CASP3, CASP6, CASP7, Bax,...

The potential protective effect of (±)-8-methoxy-1,3,4,4a,5,9b-hexahydro-pyrido[4,3-b]indole-2-carboxylic acid ethyl ester (II) was assessed against hydrogen peroxide (H2O2)-cytotoxicity in rat pancreatic INS-1E β cells and compared with the related pyridoindole, stobadine (I), a promising indole-type reference antioxidant. Only pre-treatment compound led to significant preservation metabolic secretory functions exposed H2O2. caspase-9 -3 activities, as well early apoptotic changes plasma...

BACKGROUND Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for brain cancers. OBJECTIVE To establish primary cell cultures excised tissue from xanthoastrocytoma–bearing patients test their against various anticancer chemotherapy drugs. METHODS Prepared suspensions...

The involvement of CD43 in cell proliferation murine intestinal intraepithelial lymphocytes (IEL) has been studied vitro CD3‐stimulated cultures. In the presence either IL‐2 or IL‐15, CD3 stimulation IEL resulted low levels as measured by thymidine incorporation, whereas no occurred upon absence cytokines. combination both cytokines to cultures synergistically enhanced CD3‐induced approximately threefold that supplemented with cytokine alone. Most importantly, however, was significantly...