A5019063960- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Plant and Fungal Interactions Research
- Environmental DNA in Biodiversity Studies
- Marine Bivalve and Aquaculture Studies
- Marine Toxins and Detection Methods
- Aquaculture disease management and microbiota
- Diatoms and Algae Research
- Plant Disease Resistance and Genetics
- Aquatic Ecosystems and Phytoplankton Dynamics
- RNA Research and Splicing
- Animal Virus Infections Studies
- Aquatic Invertebrate Ecology and Behavior
- Wastewater Treatment and Nitrogen Removal
- Cephalopods and Marine Biology
- Microbial infections and disease research
- RNA and protein synthesis mechanisms
- Marine Biology and Ecology Research
- Genomics and Chromatin Dynamics
- Isotope Analysis in Ecology
- Metabolomics and Mass Spectrometry Studies
Japan Fisheries Research and Education Agency
2015-2024
Saga University
2021
National Agriculture and Food Research Organization
2007
Ehime University
1999-2005
RIKEN
2005
Yokohama City University
2005
Japan Society for the Promotion of Science
2003-2004
Otsuka (Japan)
2000
Kyoto University
2000
Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of corresponding transcripts (RNA interference), as well silencing at chromatin level. Global transcriptome analysis provides evidence that large proportion genome can produce both strands, and antisense commonly link neighboring "genes" complex loci into chains linked transcriptional units. Expression profiling reveals frequent...
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There an international collaborative effort to identify all full-length mRNA transcripts from mouse, and ensure that each represented in physical collection clones. Here we report manual annotation 60,770 complementary DNA sequences. These are clustered 33,409 'transcriptional units', contributing 90.1% newly established transcriptome database. Of these transcriptional units, 4,258 new...
ABSTRACT A novel single-stranded RNA (ssRNA) virus specifically infecting the bloom-forming diatom Rhizosolenia setigera ( R. [RsRNAV]) was isolated from Ariake Sea, Japan. Viral replication occurred within cytoplasm, and particle icosahedral, lacked a tail, 32 nm in diameter on average. The major nucleic acid extracted RsRNAV particles an ssRNA molecule 11.2 kb length, although smaller molecules (0.6, 1.2, 1.5 kb) were occasionally observed. structural proteins of 41.5, 41.0, 29.5 kDa....
ABSTRACT We isolated a cyanophage (Ma-LMM01) that specifically infects toxic strain of the bloom-forming cyanobacterium Microcystis aeruginosa . Transmission electron microscopy showed virion is composed anisometric head and tail complex consisting central tube contractile sheath with helical symmetry. The morphological features host specificity suggest Ma-LMM01 member cyanomyovirus group. Using semi-one-step growth experiments, latent period burst size were estimated to be 6 12 h 50 120...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 34:207-218 (2004) - doi:10.3354/ame034207 Isolation and characterization of two distinct types HcRNAV, a single-stranded RNA virus infecting bivalve-killing microalga Heterocapsa circularisquama Yuji Tomaru1, Noriaki Katanozaka2,3, Kensho Nishida1, Yoko Shirai1, Kenji Tarutani1, Mineo Yamaguchi1, Keizo Nagasaki1,*...
ABSTRACT Cyanobacteria and their phages are significant microbial components of the freshwater marine environments. We identified a lytic phage, Ma-LMM01, infecting Microcystis aeruginosa , cyanobacterium that forms toxic blooms on surfaces lakes. Here, we describe first sequenced cyanomyovirus genome Ma-LMM01. The linear, circularly permuted, terminally redundant has 162,109 bp contains 184 predicted protein-coding genes two tRNA genes. exhibits no colinearity with previously genomes...
ABSTRACT Diatoms are a major phytoplankton group that play important roles in maintaining oxygen levels the atmosphere and sustaining primary nutritional production of aquatic environment. Among diatoms, genus Chaetoceros is one most abundant widespread. Temperature, climate, salinity, nutrients, predators were regarded as factors controlling abundance population dynamics diatoms. Here we show viral infection can occur should therefore be considered potential mortality source. salsugineum...
Diatoms are important components of the biological community and food web in aquatic environment. Here, we report characteristics a single-stranded RNA (ssRNA) virus (CtenRNAV01) that infects marine diatom Chaetoceros tenuissimus Meunier (Bacillariophyceae). The ca. 31-nm particle is icosahedral lacks tail. CtenRNAV01 forms crystalline arrays occupying most infected host's cytoplasm. By growth experiments, lytic cycle burst size were estimated to be <24 h approximately 1 x 10(4) infectious...
This is the first report of propagation toxic dinoflagellate Dinophysis fortii Pavill. under laboratory conditions when fed on marine ciliate Myrionecta rubra grown with cryptophyte Teleaulax amphioxeia (W. Conrad) D. R. A. Hill. In contrast, reduced growth (max. 3-4 divisions) and formation small cells were observed in absence or provided T. only as prey, showing that cannot utilize prey. TEM observation cells, which had fully well-developed chloroplasts (5-12 μm length) seen three...
ABSTRACT Diatoms are very significant primary producers in the world's oceans. Various environmental factors affect depletion of diatom populations. The importance viruses as a potential mortality source has recently been recognized. We isolated and characterized new virus ( Chaetoceros socialis f. radians RNA [CsfrRNAV]) causing lysis bloom-forming species Lauder (Schütt) Proschkina-Lavrenko. infectious to C. was from water samples collected Hiroshima Bay. Here we show physiology,...
ABSTRACT Recent studies have suggested that diatom viruses are an important factor affecting population dynamics, which in turn considering marine primary productivity. The planktonic Chaetoceros tenuissimus Meunier is a cosmopolitan species and often causes blooms off the western coast of Japan. To date, two viruses, C. DNA virus (CtenDNAV) type I CtenRNAV I, been identified potentially affect dynamics natural environment. In this study, we successfully isolated characterized additional...
Recent studies have suggested that the viral lysis of microbes not only facilitates conversion particulate organic matter into dissolved matter, but also promotes formation aggregates, which enhance export carbon from surface ocean to deep sea. However, experimental data supporting this proposition are limited. Here, we tested hypothesis infection marine diatoms enhances aggregate formation. We used a model system consisting Chaetoceros tenuissimus, bloom-forming diatom with an approximate...
ABSTRACT Heterocapsa circularisquama RNA virus (HcRNAV) has at least two ecotypes (types UA and CY) that have intraspecies host specificities which are complementary to each other. We determined the complete genomic sequence of typical HcRNAV strains, HcRNAV34 HcRNAV109, one ecotype. The nucleotide sequences viruses were 97.0% similar, had open reading frames (ORFs), ORF-1 coding for a putative polyprotein having protease RNA-dependent polymerase (RdRp) domains ORF-2 encoding single major...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 50:103-112 (2008) - DOI: https://doi.org/10.3354/ame01170 Isolation and characterization of a new single-stranded DNA virus infecting cosmopolitan marine diatom Chaetoceros debilis Yuji Tomaru1,*, Yoko Shirai1, Hidekazu Suzuki2, Tamotsu Nagumo3, Keizo Nagasaki1 1Harmful Algal Bloom Division, National Research Institute...
Summary We studied the ecological relationships between bloom‐forming dinoflagellate Heterocapsa circularisquama and its infectious viruses in field surveys conducted western Japan. The occurrence of H. blooms Imari Bay during 2002 Ago 2004 was accompanied by specific increase abundance lytic to . Using northern dot‐blot analysis, ∼96% clonal virus isolates collected positively reacted with a molecular probe for HcRNAV ( RNA virus); hence, viral impacts on population observed these are...
Abstract Heterocapsa circularisquama DNA virus (HcDNAV; previously designated as HcV) is a giant (girus) with ~356-kbp double-stranded (dsDNA) genome. HcDNAV lytically infects the bivalve-killing marine dinoflagellate H. , and currently represents sole isolated from dinoflagellates, one of most abundant protists in ecosystems. Its morphological features, genome type, host range suggested that might be member family Phycodnaviridae Nucleo-Cytoplasmic Large Viruses (NCLDVs), though no...
ABSTRACT Diatoms are one of the most significant primary producers in ocean, and importance viruses as a potential source mortality for diatoms has recently been recognized. Thus far, eight different diatom infecting genera Rhizosolenia Chaetoceros have isolated characterized to extents. We report isolation novel virus (ClorDNAV), which causes lysis bloom-forming species lorenzianus , show its physiological, morphological, genomic characteristics. The free virion was estimated be ∼34 nm...
ABSTRACT Heterosigma akashiwo virus (HaV) is a large double-stranded DNA infecting the single-cell bloom-forming raphidophyte (golden brown alga) H. . A molecular phylogenetic sequence analysis of HaV polymerase showed that it forms sister group with Phycodnaviridae algal viruses. All 10 examined strains, which had distinct intraspecies host specificities, included an intein (protein intron) in their genes. The 232-amino-acid inteins differed from each other by no more than single nucleotide...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 34:219-226 (2004) - doi:10.3354/ame034219 Dynamics of Heterocapsa circularisquama (Dinophyceae) and its viruses in Ago Bay, Japan Keizo Nagasaki1,*, Yuji Tomaru1, Katsuyuki Nakanishi2, Naotsugu Hata2, Noriaki Katanozaka3,4, Mineo Yamaguchi1 1National Research Institute Fisheries Environment Inland Sea, 2-17-5 Maruishi,...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 34:227-238 (2004) - doi:10.3354/ame034227 Quantitative and qualitative impacts of viral infection on a Heterosigma akashiwo (Raphidophyceae) bloom in Hiroshima Bay, Japan Yuji Tomaru, Kenji Tarutani, Mineo Yamaguchi, Keizo Nagasaki* National Research Institute Fisheries Environment Inland Sea, 2-17-5 Maruishi, Ohno,...