A5001942473- Protist diversity and phylogeny
- Telomeres, Telomerase, and Senescence
- bioluminescence and chemiluminescence research
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Mitochondrial Function and Pathology
- Microbial Community Ecology and Physiology
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Glaucoma and retinal disorders
- RNA modifications and cancer
- Mercury impact and mitigation studies
- Biochemical and Molecular Research
- Genetics, Aging, and Longevity in Model Organisms
- Diabetes Treatment and Management
- Ophthalmology and Eye Disorders
- Adenosine and Purinergic Signaling
- Heavy Metal Exposure and Toxicity
- Marine Biology and Ecology Research
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
Fuchu Hospital
2025
National Institute of Advanced Industrial Science and Technology
2006-2024
Nippon Medical School
2018-2024
Saitama Medical University
2011-2024
Japan Science and Technology Agency
2015-2016
Kobe Pharmaceutical University
2015
University of Tsukuba
1997-2015
Suzuki (Japan)
2005-2011
Ajinomoto (Japan)
2005-2011
Kindai University
2009
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There an international collaborative effort to identify all full-length mRNA transcripts from mouse, and ensure that each represented in physical collection clones. Here we report manual annotation 60,770 complementary DNA sequences. These are clustered 33,409 'transcriptional units', contributing 90.1% newly established transcriptome database. Of these transcriptional units, 4,258 new...
The involvement of microglia in neuroinflammatory responses has been extensively demonstrated. Recent animal studies have shown that exposure to either acute or chronic stress induces robust microglial activation the brain. In present study, we investigated underlying mechanism brain by stress.We first looked at spatial distribution noradrenaline (NA)-synthesizing enzyme, DBH (dopamine β-hydroxylase), comparison with NA receptors-β1, β2, and β3 adrenergic receptors (β1-AR, β2-AR,...
The expression levels of three chitinase genes in Arabidopsis thaliana, AtChiA (class III), AtChiB I), and AtChiV IV), were examined under various stress conditions by semi-quantitative RT-PCR. Under normal growth conditions, the expressed most organs plants at all stages, whereas gene was not all. class III exclusively when exposed to environmental stresses, especially salt wound stresses. Treatment with allosamidin, which inhibits chitinases, did affect rate. Surprisingly, however, treated...
Galectin-3, a beta-galactoside-binding protein, is highly expressed in thyroid papillary carcinomas, while functional relevance of galectin-3 overexpression to the malignant phenotype remains elusive. In present study we transfected antisense cDNA into human carcinoma cell line NPA which expresses an innately high level galectin-3, and examined effect inhibition expression on transformed phenotype. There was no difference anchorage-dependent growth between clones either control or parental...
Aims: Other than ectopic expression of intestinal transcription factors, Cdx1 and Cdx2 , the molecular mechanisms underlying gastric phenotypes human stomach adenocarcinomas have yet to be clarified in detail. We reported that Sox2, an HMG‐box factor, is expressed normal mucosa down‐regulated metaplasia. Methods results: analysed mRNA levels Sox2 other differentiation markers 50 surgically resected adenocarcinomas, immunohistochemically classified into (G), gastric‐and‐intestinal (GI)‐mixed,...
Copepods are the dominant taxa in zooplankton communities of ocean worldwide. Although bioluminescence certain copepods has been known for more than a 100 years, there is very limited information about structure and evolutionary history copepod luciferase genes. Here, we report cDNA sequences 11 luciferases isolated from superfamily Augaptiloidea order Calanoida. Highly conserved amino acid residues two similar repeat were confirmed by multiple alignment all luciferases. Copepod classified...
Ezetimibe inhibits Niemann-Pick C1-like 1 (NPC1L1), an apical membrane cholesterol transporter of enterocytes, thereby reduces intestinal absorption. This treatment also increases extrahepatic reverse transport via undefined mechanism. To explore this, we employed a trans-intestinal efflux (TICE) assay, which directly detects circulation-to-intestinal lumen 3H-cholesterol transit in cannulated jejunal segment, and found increase TICE by 45%. examine whether such occurs at the brush border...
Aims: Abnormal localization of β‐catenin is frequently observed in human gastric cancers. The aim the present study was to evaluate relationships among gastrointestinal differentiation phenotypes, and mutations Wnt signalling genes. Methods results: Seventy‐seven regions 39 adenocarcinomas were classified according intestinal phenotypes. Cases with membranous showed a gradual decrease from (G) (55% = 6/11) gastric‐and‐intestinal‐mixed (GI) (17% 5/29) (I) (0% 0/21) while those nuclear...
Background Colonization of extended-spectrum beta-lactamase (ESBL)-producing organisms is increasing becoming more frequent not only in hospitalized patients but also healthy individuals. Although these bacteria are thought to be transmitted newborns on their way through the birth canal, molecular evidence for this scarce. In study, we aimed survey current prevalence resistant bacterial colonization area by examining carriage organism before and after delivery. Methods We examined rate...
Polyacrylamide gel electrophoresis (PAGE) has traditionally been used to analyze lipoproteins in human and animal sera. Most conventional PAGE systems for lipoprotein analysis use disc electrophoresis, which limits the number of samples simultaneously analyzed does not allow comparison electrophoretic profiles multiple under same conditions. Other methods, such as high performance liquid chromatography agarose are also analysis; however, they require specialized equipment expertise. Here, we...
Cortisol is a classical biomarker for the stress levels of human beings. We fabricated highly sensitive bioluminescent probes salivary cortisol. The following strategies were contrived in molecular design. Gaussia princeps luciferase (GLuc) was dissected into two fragments, between which an N-terminal-extended ligand binding domain glucocorticoid receptor (GR HLBD), named Simgr4, inserted. First, this unique single-chain probe then situated downstream response element (GRE) promoter...
Peroxisome proliferator-activated receptors (PPARs) are nuclear that regulate expression of a number genes associated with the cellular differentiation and development. Here, we show abundant ubiquitous newly identified splicing variant mouse Pparγ (Pparγ1sv) encodes PPARγ1 protein, its importance in adipogenesis. The novel has unique 5'-UTR sequence, relative to those Pparγ1 Pparγ2 mRNAs, indicating presence transcriptional initiation site promoter for expression. Pparγ1sv was highly...
Proteolytic activity declines with age, resulting in the accumulation of aggregated proteins aged organisms. To investigate how disturbance proteostasis causes cellular senescence, we developed a stress-induced premature senescence (SIPS) model, which normal human fibroblast MRC-5 cells were treated proteasome inhibitor MG132 or vacuolar-type ATPase bafilomycin A1 (BAFA1) for 5 days. Time-course studies revealed significant increase intracellular reactive oxygen species (ROS) and...
Abstract: We previously reported a novel compound called S-nitroso- N -pivaloyl- d -penicillamine (SNPiP), which was screened from group of nitric oxide donor compounds with basic chemical structure -acetylpenicillamine, to activate the nonneuronal acetylcholine system. SNPiP-treated mice exhibited improved cardiac output and enhanced diastolic function, without an increase in heart rate. The acetylcholine–activating effects included increased resilience ischemia, modulation energy...
The fluorescence excitation and emission maxima of a GFP-like protein from the marine copepod Chiridius poppei (CpYGFP) show significant red shift (lambda(ex) = 509 nm, lambda(em) 517 nm) compared with those GFP Aequorea victoria (avGFP) other proteins copepods. We performed crystallographic biochemical studies to understand why this occurs in CpYGFP. structure CpYGFP showed that imidazole side chain His52 is involved stacking on phenol moiety chromophore. investigated potential role causing...
During previous work on deriving inosine-producing mutants of Escherichia coli, we observed that an excess adenine added to the culture medium was quickly converted hypoxanthine. This phenomenon still apparent after disruption known adenosine deaminase gene (add) E. coli chromosome, suggesting that, like Bacillus subtilis, has deaminase. As yicP shares about 35% identity with B. subtilis (ade), cloned from genome and developed a strain overexpressed its product. The enzyme purified cell...