A5027603921- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Adrenal Hormones and Disorders
- Immunotherapy and Immune Responses
- Biomedical Ethics and Regulation
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Stress Responses and Cortisol
- Dermatology and Skin Diseases
- Chemokine receptors and signaling
- Advanced Biosensing Techniques and Applications
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- 3D Printing in Biomedical Research
- Hormonal Regulation and Hypertension
Nantes Université
2018-2023
Inserm
2018-2023
Center for Research in Transplantation and Translational Immunology
2020-2023
Centre National de la Recherche Scientifique
2021-2022
Institut de Transplantation Urologie en Nephrologie
2018-2020
Centre d'Investigation Clinique de Nantes
2020
Centre Hospitalier Universitaire de Nantes
2020
Human trophoblast stem cells (hTSCs) derived from blastocysts and first-trimester cytotrophoblasts offer an unprecedented opportunity to study the placenta. However, access human embryos placentas is limited, thus preventing establishment of hTSCs diverse genetic backgrounds associated with placental disorders. Here, we show that can be generated numerous using post-natal via two alternative methods: (1) somatic cell reprogramming adult fibroblasts OCT4, SOX2, KLF4, MYC (OSKM) (2) fate...
Abstract Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of embryonic origin offer potential to generate patient-specific cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, iPSCs (hiPSCs), restricting their post-implantation development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we...
Abstract Over a decade after their discovery, induced pluripotent stem cells (iPSCs) have become major biological model. The iPSC technology allows generation of from somatic bearing any genomic background. challenge ahead us is to translate human iPSCs (hiPSCs) protocols into clinical treatment. To do so, we need improve the quality hiPSCs produced. In this study report reprogramming multiple patient urine-derived cell lines with mRNA reprogramming, which, date, one fastest and most...
Abstract Autoimmune regulator (AIRE) deficiency in humans induces a life-threatening generalized autoimmune disease called polyendocrinopathy–candidiasis–ectodermal dystrophy (APECED), and no curative treatments are available. Several models of AIRE-deficient mice have been generated, although they useful understanding the role AIRE central tolerance, do not reproduce accurately APECED symptoms, thus there is still need for an animal model displaying APECED-like disease. We assessed, this...
Targeted monoclonal antibody (mAb) therapies show great promise for the treatment of transplant rejection and autoimmune diseases by inducing more specific immunomodulatory effects than broadly immunosuppressive drugs routinely used. We recently described therapeutic advantage targeting CD45RC, expressed at high levels conventional T (Tconv) cells (CD45RChi), their precursors, terminally differentiated (TEMRA) cells, but not regulatory (Tregs; CD45RClo/-). demonstrated efficacy anti-CD45RC...
Multiple sclerosis (MS) is a chronic inflammatory and demyelinating disease of the CNS. Studies immune dysfunction in MS have mostly focused on CD4+ Tregs, but role CD8+ Tregs remains largely unexplored. We previously evidenced suppressive properties rat human CD8+CD45RClow/neg from healthy individuals, expressing Forkhead box P3 (FOXP3) acting through interferon-gamma (IFN-γ), transforming growth factor beta (TGFβ), interleukin-34 (IL-34). secretions to regulate responses control diseases...
Cell therapy using T cells has revolutionized medical care in recent years but limitations are associated with the difficulty of genome editing cells, production a sufficient number and standardization product. Human pluripotent stem (hPSCs) can self-renew differentiate into to provide standardized homogenous product defined origin indefinite quantity, therefore they great potential alleviate therapeutic cell production. The differentiation hPSCs takes place two steps: first induction...
Immunotherapy using primary T cells has revolutionized medical care in some pathologies recent years, but limitations associated to challenging cell genome edition, insufficient number production, the use of only autologous cells, and lack product standardization have limited its clinical use. The alternative generated vitro from human pluripotent stem (hPSCs) offers great advantages by providing a self-renewing source that can be readily genetically modified facilitate standardized...
The success of inducing human pluripotent stem cells (hIPSC) offers new opportunities for cell-based therapy. Since B exert roles as effector and regulator immune responses in different clinical settings, we were interested generating from hIPSC. We differentiated embryonic (hESC) hIPSC into onto OP9 MS-5 stromal successively. overcame issues CD34 + CD43 hematopoietic progenitors with appropriate cytokine conditions emphasized the difficulties to generate proper progenitors. highlight CD31...
SUMMARY Human trophoblast stem cells (hTSC) derived from blastocysts and first-trimester cytotrophoblasts offer an unprecedented opportunity to study the human placenta. However, access embryos first trimester placentas is limited thus preventing establishment of hTSC a variety genetic backgrounds associated with placental disorders. In present study, we show that can be generated numerous using post-natal via two alternative methods: (I) somatic cell reprogramming adult fibroblasts Yamanaka...
ABSTRACT Immunotherapy using primary T cells has revolutionized medical care in some pathologies recent years but limitations associated to challenging cell genome edition, insufficient number production, the use of only autologous and lack product standardization have limited its uses clinic. The alternative generated vitro from human pluripotent stem (hPSCs) offers great advantages by providing a self-renewing source that can be readily genetically modified facilitate standardized...
ABSTRACT Although described in the 70’s, CD8 + regulatory T cells (Tregs) remain incompletely understood and to date, although several markers are used define them, they poorly defined. The identification of reliable consistent markers, as it was done for CD4 Tregs, remains an urgent task a challenge advance our understanding. Herein, we analyzed total using single cell CITEseq VDJ receptor sequencing utilizing previously identify particular CD45RC by team others divide pro-inflammatory...
One major goal in transplantation is to develop novel specific and non-toxic anti-rejection immunotherapies. Strategies based on regulatory T cells (Tregs) are promising prevent graft rejection. Our group has shown that rat human CD8+CD45RClow/- Tregs display significant suppressive function. The team also cell therapy using was efficient rejection GVHD humanized NSG mice models. However, the heterogeneity of population important from a phenotypic point view, suggesting either fraction...
Abstract Immune homeostasis requires fully functional Tregs with a stable phenotype to control autoimmunity. Although IL-34 is cytokine first described as mainly involved in monocyte cell survival and differentiation, we recently its expression by CD8+ rat model of transplantation tolerance activated FOXP3+ CD4+ human healthy individuals. However, role autoimmunity potential diseases remain be determined. Here report that the absence Il34 -/- rodents leads an unstable phenotype, production...
CD8+ regulatory T cells (Tregs) were the first suppressive reported in 1970, but they put aside for years due to a lack of markers properly define them. Our team demonstrated that Tregs identified by low and/or negative expression CD45RC, one isoforms CD45 molecule, show potent activity vitro and vivo, while expressing high levels CD45RC do not. Herein, we addressed heterogeneity within CD8+CD45RClow/- new markers. We performed single cell RNA-sequencing on more than 10000 non-stimulated 3...