This research was the first to demonstrate changes in nutritional compositions (isoflavone and CLA) from 50% methanol extracts of soy-powder milk (SPM) yogurt (SPY) through fermentation using Lactobacillus plantarum S48 P1201 strains. The radical scavenging activities protective effects against oxidative stress LLC-PK1 cells were also investigated. average physicochemical characteristics including acidity viable cell number as well β-glucosidase activity increased with 0.2 → 0.7%, 7.5 9.8...

The biochemical modification using microorganisms had an excellent functional values. This research was the first to investigate changes in nutritional components (ginsenosides, flavonols, and phenolic acids) biological properties (antioxidant anti-inflammatory) of mountain-cultivated ginseng sprouts (MCGS) during aging (AMCGS) fermentation (FAMCGS) processes. Total ginsenosides were considerably reduced (45.1 → 4.9 31.7 mg/g) 50% ethanol extracts processed sources, while flavonol acid...

Chlorpyrifos (CP) residues are absorbed from soil and often found in Korean cabbages that being used to make kimchi. Lactobacillus sakei WCP904, harboring the organophosphorus (OP) hydrolase gene opdD, was isolated CP-impregnated mulkimchi. The cloned opdD strain CP904 comprises 825 base-pair nucleotides encode 274 amino acids. recombinant Escherichia coli depleted 73% of CP after 6 days M9 medium. In fact, OpdD protein is a novel member GHSQG family esterolytic enzymes or lactic acid...

This study evaluated the changes in γ-aminobutyric acid (GABA) and isoflavone aglycone contents from soy powder yogurt (SPY) due to sprouting of soybean (1 cm) fermentation with Lactobacillus brevis. The levels GABA form increased, glutamate decarboxylase β-glucosidase activities increased; however, glycoside malonylglycoside decreased after for 72 h. In particular, 60 h, SPY presented highest content (120.38 mg/100 mL). daidzein (179.93 µg/g), glycitein (44.10 genistein (126.24 µg/g) were...

Seung Ha Hwang · Su Cheol Kim Jin A Seong Hee Yul Lee Du Yong Cho Min Ju Jea Gack Jung Eun Hye Jeong Ki-Ho Son Kye Man Cho. JABC 2021;64:253-61. https://doi.org/10.3839/jabc.2021.035

This study investigated the changes in physicochemical properties, total phenolic and phytoestrogen (isoflavone) contents, antioxidant activity, including 2,2-diphenyl-1-picrydrazyl (DPPH) 2,4,6-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical activities ferric reducing power (FRAP), during lactic acid fermentation of soy powder milk (SPM) with colored small soybean (CSS) by potential probiotic Lactobacillus plantarum P1201. The pH decreased, while viable cell numbers...

In this study, soy-powder yogurt (SPY) with enhanced levels of γ-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were (72 h) Lactobacillus brevis, the total free amino acids (FAAs) formed mixtures determined be 79.53, 489.93, 877.55, 780.53, 979.97 mg/100 mL in HPS (FHPS), 0 cm (FSHPS-0), 1 (FSHPS-1), 2 (FSHPS-2), lengths (FSHPS-4), respectively. glutamic (GA) GABA observed highest, 100.31...

Achyranthes japonica Nakai (AJN) is grown mainly in the warm regions of South Korea. It a high-value medicinal plant with various pharmacological effects, including joint protection, obesity prevention, and diabetes prevention. AJN valued for its roots, it takes approximately two years from sowing before roots can be harvested. Therefore, this study sought to develop better cultivation technology microgreen form vertical farm confirm bioactive compounds antioxidant capacity according amount....

This study was the first to document fluctuations of nutritional constituents, antioxidant capacities, and physicochemical characteristics during aging processes red ginseng sprouts (RGS) black (BGS) from dried (DGS). Total ginsenoside levels differed with 54.72 (DGS) → 57.15 6.98 mg/g, specifically, F2 Rd2 in RGS remarkably increased 1.97 5.88 2.41 5.49 respectively. Phenolics dramatically as 297.02 1770.01 (6.0-fold); 1834.94 (6.2-fold) μg/g DGS RGS; BGS abundance contents benzoic acid...

This study aimed to produce fermented soy-powder milk (FSPM) with Lactobacillus plantarum P1201 and evaluate its anti-obesity activity. Isoflavone conjugated linoleic acid (CLA) of unfermented (UFSPM) FSPM were analyzed via high-pressure liquid chromatography (HPLC) gas (GC). Their inhibitory activities against α-glucosidase, α-amylase, pancreatic lipase assayed. evaluated on the basis their effects adipocyte differentiation in 3T3-L1 cells, expression mRNAs associated adipogenesis lipid...

In this study, contetnts of phenolic acid and isoflavone, biological activities soy sauce were compared the added bitter melon powder (BMPs). After fermentation, pHs decreased from 5.83 (0% BMP), 5.47 (5% 5.32 (10% BMP) to 5.28, 5.36, 5.16 at 90 days, whereas acidities increased 0.06%, 0.07%, 0.09% 0.30%, 0.28%, 0.36% respectively. addition, salinities decreased, while viable cell numbers including Bacillus yeast increased. The contents total phenolic, isoflavone-aglycone, antioxidant...

This study comprehensively investigated the physicochemical properties, nutritional constituents, and antioxidant activity of ginseng sprouts (GS) according to roasting process. The fatty acid mineral contents GS were almost unchanged, but free amino decreased. Moreover, ginsenoside decreased during process, whereas total phenolics flavonoids, Maillard reaction products, phenolic flavonol increased. In particular, Re, Rb1, Rd by 41%/18%, 32%/30%, 13%/23% (above/below ground), respectively,...

The research was aimed to analyze the functional constituents (GABA and isoflavone), radical (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl) scavenging activities enzyme (α-glucosidase lipase) inhibitory effects of soypowder milk (SPM) fermented soy-powder (FSPM) with varied Lactobacillus brevis. Ten γ-aminobutyric acid (GABA) producing lactic bacteria that showed 96-99% similarity L. brevis, according 16S rRNA gene sequence...

In this study, vineger was produced after heat treatment of Elaeagnus multiflora juice and its fermentative characteristics were investigated. The heat-treated vinegar E. similar in fruit color, with b values (redness) 39.48 (juice) 37.56 (vinegar). After 10 days fermentation fruit, the acetic acid bacteria viable cell number, pH, acidity, reducing sugar content, alcohol content 4.59-4.62 log CFU/mL, 3.14-3.45, 0.2-2.12%, 0.69-35.24 mg/mL, 0.2%, respectively. showed significantly higher...

느티만가닥버섯 수확후배지 발효사료 급여가 산란계에 미치는 영향을 조사하기 위하여 도준농산에서 수거한 수확후배지를 Bacillus subtilis EJ3 배양액과 혼합하여 2주 동안 발효시킨 것을 공시사료로 사용하였다. 실험동물인 12주령된 Hy-line brown 갈색계 24수는 시판사료 급여구인 대조구(T0)와 5(T1), 10(T2), 15%(T3)의 버섯수확후배지 발효산물이 첨가된 시험구로 나누어 12주 공시사료를 급여하였다. 12주동안 평균 산란율은 대조구에 비해 5% 발효산물 첨가구에서 증가하였다가 첨가량이 증가할수록 감소하였다. 사료섭취량은 대조구와 첨가구에서는 큰 차이를 보이지 않았지만 약간 증가하였다. 사료요구율은 버섯수확후 배지 비슷하게 나타났으며 10% 첨가구부터는 높게 나타났다. 산란량과 대조구보다는 총 12주의 사양시험기간 난중, 난각강도, 난각두께, 난황색 모두 발효산물을 첨가한 처리구에서는 차이가 없었지만 발효산물의 감소하는 경향을 나타내었다. 계란의...

This study investigated that change of the nutrients (including fatty acids, amino and minerals) total phenolic (TP), flavonoid (TF), isoflavone contents antioxidant activities during storage soybean leaves containing high at 5, 25, 55 o C for 180 days.The free acids were increased from 1313.81 mg/100 g 0 day to 1776.15, 1693.93 1551.18mg/ 100 days, respectively, but values little unchanged.The minerals detected 51.65 (0 day) 49.93 (5 C), 50.20 (25 61.21 (55 C) respectively.The levels...

This study investigated that comparison of the nutrients (including fatty acids, amino and minerals) ginsenoside, total phenolic (TP) flavonoid (TF) contents antioxidant activities in 5-year-old cultivated ginseng (CG), mountain-cultivated (MCG), whole plant parts MCG (WPMCG).The acids unsaturated were determined highest (1215.9 751.2 mg/100 g), saturated acid was WPMCG (486.4 g) respectively.Characteristically, αlinolenic content detected up to 10 times higher (139.3 (194.4 compared CG...

This study investigated the phenolics (TP) and phytochemical contents radical-scavenging activity of ethanol extracts Dendropanax morbiferus parts (leaf, stem, root). The chlorogenic acid (CA) rutin (RT) all by were in order leaf, stem root. In case TP 2.15-2.52 mg/g when extracted with hot water concentration (30-70%), there was no difference depending on extraction solvents times. However, 95% alcohol showed lowest content (extraction time: 2-4 h). Particularly, CA RT 70% ethanolic extract...

Alzheimer’s disease (AD) is the most common cognitive disorder in elderly population. However, effective pharmacological agents targeting AD have not been developed. The processed Polygoni multiflori Radix (PPM) and its main active substance, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside (TSG), has received considerable attention, majorly due to neuroprotective activities against multiple biological within human body. In this study, we provide new evidence on therapeutic effect of PPM TSG...

Jin A Seong · Hee Yul Lee Su Cheol Kim Du Yong Cho Jea Gack Jung Min Ju Ae Ryeon Jong Bin Jeong Ki-Ho Son Kye Man Cho. JABC 2022;65:129-42. https://doi.org/10.3839/jabc.2022.018

In this study, we investigated the changes in physicochemical properties, yeast diversity, and volatile flavor compounds during fermentation of mugwort sugar extracts (FMSE). The pH sucrose levels decreased, while acidity reducing sugar, fructose, glucose contents increased FMSE. Oxalic succinic acids appeared to be predominant organic Based on 26S rRNA sequencing, a total 7 different species including Candida lactis-condensi, Hanseniaspora uvarum, Hyphopichia burtonii, Saccharomyces...

본 연구는 큰느타리버섯의 베타글루칸 고함유 형질에 관련된 SCAR marker를 개발하기 위해 수행되었다. operon사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 대립 계통 9종과 9 종을 대상으로 RAPD를 이용한 bulked segregant analysis를 실시하여 OP-R03 primer로부터 계통에는 나타나지 않고 계통에만 나타나는 특이적인 RAPD 밴드를 얻었다. 결과, 약 91 bp 부근에서 계통에 DNA 밴드가 관찰되었으며 이 밴드의 염기서열 말단을 근거로 마커로 사용할 specific primer인 OP-R03-1-F와 OP-R03-1-R를 디자인하였다. 마커 OP-R03-1-F/-1-R 이용하여 PCR을 수행한 결과에서도 계통과 구별되는 계통에서 확인되었으며 수행했을 때보다 재현성이 높고 진한 밴드임을 확인할 수...