A5003135717- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Renal Diseases and Glomerulopathies
- Cytomegalovirus and herpesvirus research
- Transplantation: Methods and Outcomes
- Complement system in diseases
- Renal and Vascular Pathologies
- Organ Donation and Transplantation
- Neurological Complications and Syndromes
- Immune Cell Function and Interaction
- Blood groups and transfusion
- Dialysis and Renal Disease Management
- Polyomavirus and related diseases
- Chronic Kidney Disease and Diabetes
- Metabolism and Genetic Disorders
- Diabetes and associated disorders
- Liver Disease and Transplantation
- Plant tissue culture and regeneration
- Vasculitis and related conditions
- Systemic Lupus Erythematosus Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Genetic and Kidney Cyst Diseases
- Plant Reproductive Biology
Institute of Clinical and Experimental Medicine
2015-2024
Charles University
2016-2018
Czech Academy of Sciences, Institute of Experimental Botany
1999-2005
Czech Academy of Sciences
2004
Rabbit-generated antithymocyte globulins (ATGs), which target human T cells, are widely used as immunosuppressive agents during treatment of kidney allograft recipients. However, ATGs can induce immune complex diseases, including serum sickness disease (SSD). Rabbit and IgGs have various antigenic differences, expression the sialic acid Neu5Gc α-1-3-Gal (Gal), not synthesized by beings. Moreover, anti-Neu5Gc antibodies been shown to preexist be elicited immunization in subjects. This study...
Asymptomatic cytomegalovirus (CMV) infection is associated with graft dysfunction and failure. However, no study assessed CMV viral load in terms of the risk for failure.In a prospective cohort kidney transplant recipients, we impact DNAemia on overall survival incidence moderate-to-severe interstitial fibrosis tubular atrophy (IF/TA) protocol biopsy at 36 months. was stratified by whole blood.A total 180 patients transplanted from October 2003 through January 2011 were included followed 4...
Presensitized patients with donor-specific antibodies (DSAs) are at increased risk of antibody-mediated rejection (AMR) kidney allografts.1 The long-term consequences AMR serious because currently available therapeutic options lack lasting effectiveness.2 Targeting plasma cells to counter antibody production may hold promise for treating AMR.3 Daratumumab is a fully humanized monoclonal directed against CD38, glycoprotein expressed high levels on and, in addition, natural killer cells, which...
The aim of the study was to characterize by molecular profiling two glomerular diseases: IgA nephropathy (IgAN) and focal segmental glomerulosclerosis (FSGS) identify potential markers IgAN FSGS progression. expressions 90 immune-related genes were compared in biopsies patients with (n=33), (n=17) controls (n=11) using RT-qPCR. To disease progression, gene expression between progressors non-progressors 1 year follow-up. results verified on validation cohort (n=8) (n=6) laser-capture...
Induction therapy can improve kidney transplantation (KTx) outcomes, but little is known about the mechanisms underlying its effects.The mRNA levels of T cell-related genes associated with tolerance or rejection (CD247, GZMB, PRF1, FOXP3, MAN1A1, TCAIM, and TLR5) lymphocyte subpopulations were monitored prospectively in peripheral blood 60 transplant recipients before 7, 14, 21, 28, 60, 90 days, 6 months, 12 months after KTx. Patients treated calcineurin inhibitor-based triple...
Cytomegalovirus (CMV) infection is associated with allograft rejection but the mechanisms behind are poorly defined yet. Although cross-reactivity of T cells to alloantigen and CMV has been hypothesised, direct evidence in patients lacking. In this observational cohort study, we tested pre-transplant effector/memory cell response peptide pools 78 living donor/recipient pairs using interferon-gamma Enzyme-Linked ImmunoSpot (ELISPOT) assay. To prove hypothesis cross-reactivity, analysed by...
Cytomegalovirus (CMV) prophylaxis may prevent CMV indirect effects in renal transplant recipients. This study aimed to compare the efficacy of valganciclovir and valacyclovir for after transplantation with focus on chronic histologic damage within graft. From November 2007 through April 2012, adult recipients were randomized, an open-label, single-center study, at a 1:1 ratio 3-month (n = 60) or 59). The primary endpoint was moderate-to-severe interstitial fibrosis tubular atrophy assessed...
Background: The complement system activation and regulation have been linked to post-transplant pathologies including chronic antibody mediated rejection (cAMR) the recurrence of IgA nephropathy (ReIgAN) but distinct mechanisms remain be elucidated. Methods: In this retrospective single center study, outcome kidney transplantation was studied in 150 patients with late histological diagnosis either cAMR or ReIgAN, 14 stable grafts at 3 months finally 11 native IgAN nephropathy. To study a...
The recurrence of IgA nephropathy (IgAN) after kidney transplantation occurs in 20–35% patients. main aim this study is to evaluate risk factors affecting the course IgAN renal biopsy native and transplant. We evaluated clinical parameters histological findings at time 313 patients with a follow-up up 36 years. Using hierarchical clustering method, graft failure (n=50) were divided into two groups based on mean from transplant (11.2 versus 6.1 years). time-to-graft corresponded well...
Intimal arteritis is known to be a negative prognostic factor for kidney allograft survival. Isolated v-lesion (IV) defined as intimal with minimal tubulointerstitial inflammation (TI). Although the Banff classification assesses IV T cell-mediated rejection (TCMR), clinical, and significance of early (early IV, eIV) C4d donor-specific antibodies (DSA) remains unclear. To help resolve if such eIV truly represents acute rejection, molecular study was performed. The transcriptome (n=6),...
Accommodation in ABO-incompatible (ABOi) transplantation and subclinical antibody-mediated rejection HLA-incompatible (HLAi) share several morphological similarities. Because the clinical long-term outcomes differ, we hypothesized different molecular processes involved ABOi rejection.Using Illumina Human HT-12 v4 Expression BeadChips, whole transcriptome was evaluated based on 3-month protocol C4d+ biopsies otherwise stable HLAi kidney grafts, as well C4d-negative HLA-compatible grafts...
Long-term peritoneal dialysis (PD) is associated with functional and structural alterations of the membrane. Inflammation may be key moment, and, consequently, fibrosis end result chronic inflammatory reaction. The objective present study was to identify genes involved in during PD by comparing transcriptome cells patients short- long-term PD. Peritoneal effluent long dwell stable centrifuged obtain cells. gene expression profiles using microarray between were compared. Based on analysis, 31...
Complete and high-resolution (HR) HLA typing improves the accurate assessment of donor–recipient compatibility pre-transplant donor-specific antibodies (DSA). However, value this information to identify de novo immune-mediated graft events its impact on outcomes has not been assessed. In 241 donor/recipient kidney transplant pairs, DNA samples were re-evaluated for six-locus (A/B/C/DRB1/DQB1+A1/DPB1) HR typing. De anti-HLA assessed using solid-phase assays, dnDSA classified either (1) as per...
Permanent irritation of the peritoneum during peritoneal dialysis (PD) treatment leads to local chronic inflammation and subsequently activation processes driving fibrogenesis in long-term. The aim study was compare effluent transcriptome 20 patients treated less 13 more than 2 years using microarray analysis. An increased expression genes associated with an immune response observed long-term well preserved function, when compared years. From 100 highly expressed patients, a significant...
Both antibody mediated (AMR) and T-cell (TCMR) rejections either acute or chronic represent the main reason for late graft dysfunction. In this study we aimed to evaluate differences in intrarenal expression patterns of immune system related genes rejections. Graft biopsies were performed evaluated according Banff classification. Using TaqMan Low Density Array, expressions 376 relating response (B-cell activation, chemokines, growth factors, regulators, apoptosis) analyzed four rejection...