A5071225594- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Cell death mechanisms and regulation
- Hepatitis B Virus Studies
- Liver physiology and pathology
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Bariatric Surgery and Outcomes
- Parathyroid Disorders and Treatments
- Phagocytosis and Immune Regulation
- Reproductive biology and impacts on aquatic species
- Hepatitis C virus research
- Axon Guidance and Neuronal Signaling
- Testicular diseases and treatments
- Bacterial Genetics and Biotechnology
- Social Sciences and Governance
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2005-2024
Stabilité Génétique, Cellules Souches et Radiations
2012-2024
Université Paris-Saclay
2011-2024
Institut de Radiobiologie Cellulaire et Moléculaire
1996-2024
CEA Paris-Saclay
2021-2024
Université Paris Cité
2006-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2003-2024
Inserm
1993-2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2023
Université Bourgogne Franche-Comté
2021
Abstract Background Spermatogenesis in adult is a complex stepwise process leading to terminally differentiated spermatozoa. The cellular heterogeneity of testis renders the studies on molecular aspects this differentiation process. Analysis regulation spermatogenesis would undoubtedly benefit from development techniques characterize each germinal step. Methods Hoechst 33342 staining mouse testicular cells allows characterization an enriched population stem cell and spermatogonia, called...
Fibrotest (FT) and Actitest (AT) are biochemical markers of fibrosis activity for use as a non-invasive alternative to liver biopsy in patients with chronic hepatitis C virus (HCV). The aim this study was perform an external validation FT AT the discordances between FT/AT C.A total 519 consecutive HCV were prospectively included five centers, taken at same day. Fifteen excluded because their biopsies could not be interpreted. Diagnostic accuracies assessed by receiver operating...
In patients with chronic hepatitis C virus, liver biopsy is the gold standard for assessing disease stage; nevertheless, it prone to complications, some of them serious. Non-invasive methods have been proposed as surrogate markers fibrosis. It was shown that serum hyaluronic acid (HA) level increases development The aim this study evaluate diagnostic value HA well determine cut-off predicting presence or absence fibrosis, severe and cirrhosis.405 were prospectively included biomarker...
Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the 'Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular also display a express Bcrp1 mRNA, ABC transporter responsible for efflux hematopoietic cells. Inhibition of specific BCRP1 inhibitor Ko143 germinal phenotype is dependent activity. Analysis two well-defined models altered spermatogenesis (W/Wv mutants and cryptorchid male mice) RNA expression studies...
Continuous or cyclic production of spermatozoa throughout life in adult male vertebrates depends on a subpopulation undifferentiated germ cells acting as spermatogonial stem (SSCs). What makes these self-renew differentiate is barely understood, particular nonmammalian species, including fish. In the highly seasonal rainbow trout, at end annual spermatogenetic cycle, tubules spawning testis contain only spermatozoa, with exception scarce spermatogonia that remain tubular wall and will...
Anti-silencing function 1 (ASF1) is an evolutionarily conserved histone H3-H4 chaperone involved in the assembly/disassembly of nucleosome and modification. Two paralogous genes, Asf1a Asf1b, exist mouse genome. ubiquitously expressed its loss causes embryonic lethality. Conversely, Asf1b expression more restricted has been less studied. To determine vivo we generated a Asf1b-deficient line (Asf1b(GT(ROSA-βgeo)437)) which lacZ reporter gene driven by promoter. Analysis β-galactosidase...
// Sébastien Corbineau 1, 2, 3, 4 , Bruno Lassalle Maelle Givelet 4, 7, 8, 9 Inès Souissi-Sarahoui 6 Virginie Firlej Paul Henri Romeo Isabelle Allemand 5 Lydia Riou Pierre Fouchet 1 CEA DRF iRCM SCSR, Laboratoire de Recherche sur la réparation et Transcription dans les cellules Souches, UMR 967, F-92265 Fontenay-aux-Roses, France 2 INSERM, UMR967, 3 Université Paris Diderot, Sorbonne Cité, Sud, Gamétogenèse, Apoptose Génotoxicité, Radiopathologie, 7 INSERM U1016, Institut Cochin, 75014, 8...
Fanconi anemia (FA) is a human rare genetic disorder characterized by congenital defects, bone marrow (BM) failure and predisposition to leukemia. The progressive aplastic suggests defect in the ability of hematopoietic stem cells (HSC) sustain hematopoieis. We have examined role nuclear FA core complex gene Fancg functionality HSC. In Fancg−/− mice, we observed decay long-term HSC multipotent progenitors that account for reduction LSK compartment containing primitive cells. lymphoid myeloid...
Abstract Testis is one of the organs with most telomerase activity in adult. This protects chromosomes from telomere attrition and ensures transmission full-length to progeny. Little known about during adult germ cell differentiation, however. We demonstrate here that mouse testis resides α6-integrin-positive Side Population containing spermatogonia enriched spermatogonial stem cells. The these cells fell upon entry into meiosis subsequent spermiogenesis. In addition, various stages...
The stem cell niche plays a crucial role in the decision to either self-renew or differentiate. Recent observations lead hypothesis that O 2 supply by blood and local tension could be key components of testicular spermatogonial cells (SSCs). In this study, we investigated impact different hypoxic conditions (3.5%, 1%, 0.1% tension) on murine human SSCs culture. We observed deleterious effect severe hypoxia (1% ) capacity form germ clusters when plated at low density. Severe effects...
Spermatogonia- stem cells and progenitors of adult spermatogenesis- are killed through a p53-regulated apoptotic process after γ-irradiation but the death effectors still poorly characterized. Our data demonstrate that both intrinsic extrinsic pathways involved, especially spermatogonia can be split into two main populations, according to effectors. Following irradiation Dr5 Puma genes upregulated in α6-integrin-positive Side Population (SP) fraction, which is highly enriched spermatogonia....
L’influence de la présence femmes dans les conseils d’administration sur RSE est complexe à établir. La disparité cette influence selon variétés capitalisme (VoCs) pourrait expliquer non-convergence des résultats études antérieures adoptant une perspective exclusivement nationale. En mobilisant un large échantillon européen (5 877 observations entre 2004 et 2019 provenant 17 pays), nous examinons relation scores ESG, ainsi que l’effet substitutif ou complémentaire VoCs (Liberal Market...
Fanconi anemia (FA) is a rare human genetic disorder characterized by bone marrow failure, predisposition to cancer and developmental defects including hypogonadism. Reproductive leading germ cell aplasia are the most consistent phenotypes seen in FA mouse models. We examined role of nuclear core complex gene Fancg development primordial cells (PGCs), embryonic precursors adult gametes, during fetal development. PGC maintenance was severely impaired Fancg-/- embryos. observed defect number...